Solulink/3 iCLIP Primer - (O-30050)/1/O-30050-03188bio精品生物—专注于实验室精品爆款的电商平台

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PO DNA, 5" (rApp) AGA TCG GAA GAG CGG TTC AG (ddC) 3"UV crosslinking and immunoprecipitation (CLIP) was developed to identify RNA-protein interactions (Ule J et. al. 2003). The method involves irreversible crosslinking of the RNA to protein, immunoprecipitation, ligation of 5" and 3" adapters, reverse transcription and finally PCR amplification. Unfortunately, CLIP is limited because reverse-transcriptase cannot read through crosslinked areas. This creates truncated cDNA without the 3" and 5" adapters needed to initiate PCR amplification.While several versions of the CLIP method now exist, individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) has a number of advantages. iCLIP circumvents the problem of poor amplification of the truncated cDNA. This is accomplished through the use of a preadenylated primer (iCLIP primer) and circularization of the reverse transcription product. This method also resolves the binding site to individual nucleotides and increases accuracy for binding sites located within repetitive motifs (Konig J et al. 2010., Konig J et, al. 2011).

Product Details

Catalog No	O-30050
Quality Control	Pass
Backbone	Phosphodiester
Sequence	5 (rApp) AGA TCG GAA GAG CGG TTC AG (ddC) 3
Recommended Storage	-20°C or below
Application	Next-Generation Sequencing (NGS)

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Products are for research use only, not for use in diagnostic or therapeutic procedures or for use in humans. Products are not for resale without express written permission of Seller. No license under any patent or other intellectual property right of Seller or its licensors is granted or implied by the purchase unless otherwise provided in writing.

TriLink does not warrant that the use or sale of the products delivered hereunder will not infringe the claims of any United States or other patents or patents pending covering the use of the product alone or in combination with other products or in the operation of any process. All and any use of TriLink product is the purchaser"s sole responsibility.

References

Ule, Jernej; Jensen, Kirk B.; Ruggiu, Matteo; Mele, Aldo; Ule, Aljaz; Darnell, Robert B. . CLIP identifies Nova-regulated RNA networks in the brain.

Konig, Julian; Zarnack, Kathi; Rot, Gregor; Curk, Tomaz; Kayikci, Melis; Zupan, Blaz; Turner, Daniel J.; Luscombe, Nicholas M.; Ule, Jernej . iCLIP--transcriptome-wide mapping of protein-RNA interactions with individualnucleotide resolution.

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Solulink/3 iCLIP Primer - (O-30050)/1/O-30050-03

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