GoldBio/Proteinase K, RNase/DNase free/P480100/100 mg

ProteinaseKisahighlyreactivenonspecificserineproteasethatbelongstothesubtilisinfamilyofproteins.Itcleavesatthecarboxylicacidsideofaliphatic,aromatic,orhydrophobicaminoacids.ProteinaseKiscapableofinactivatingRNasesandDNasesandisusedintheisolationorpreparationofhighmolecularweightnucleicacids.ProteinaseKisalsousefulforhelpingtocharacterizeenzymes,duetoitscleavagespecificity.ThisenzymewasdesignatedproteinaseKbecauseofitsABIlitytohydrolyzekeratin.ProteinaseKisstableinawidevarietyofdetergentsandbuffersaltsandatvarioustemperaturesandpH.TheisoelectricpointofproteinaseKis8.9.

ProteinaseKistypicallyusedataconcentrationof50-100μg/ml.ItisactivewithorwithoutthepresenceofSDS,urea,EDTAorvariousmetalions,buttheactivityofproteinaseKcanbeincreasedbyaddingthedenaturingagentsandthestructureofproteinaseKcanbestabilizedbyadditionofCa²⁺.ProteinaseKisinactivatedbyheatingto95°Cfor10minutesorusinganinhibitorsuchasPMSF,AEBSForDFP.

ProductSpecifications
ProteinaseK,RNase/DNasefree

MOLECULARBIOLOGYGRADE

MW:28.5kDa

Activity:≥30U/mg

UnitDefinition:Oneunitisdefinedastheamountofenzymethatwillliberate1.0μmoltyrosine(Folin-positiveaminoacid)fromcaseinperminuteat37°C,pH7.5.

Storage/Handling:Storeat-20°C.
Reconstitutein50mMTris-HCl(pH8.0),3mMCaCl₂.