3,3′,5,5′-Tetramethylbenzidinedihydrochloride;
TMB·HCl;
TMBdihydrochloride
MOLECULARBIOLOGYGRADE
C16H20N2·HCl·xH2O
MW:313.27g/mol(anhydrous)
TMBisanexcellentcolorimetricsubstratefordetectionofhorseradishperoxidaselabeledprobesandisusedwithperoxidaseandperoxidase-coupledsystems,particularlyinELISAtechniques.Itproducesasolubleendproduct,whichisblueincolorandcanbereadspectrophotometrically.TMBhasalsobeenusedinthequantitativedeterminationofhemoglobinandincytochemicalstainingforperoxidase.TMBisahepatitisBantigen.
Storage/Handling:Storedryat-15°C,andprotectfromlightandhumidity.