Coelenterazineisaluminophorefoundinaquaticorganismsandisthesubstrateformanyluciferaseenzymes,includingRenillareniformis,Gaussiaandaequorins.ItiscommonlyusedforbioluminescentcalciumdetectionandthemonitoringofreportergenesinBRET(BioluminescenceResonanceEnergyTransfer),ELISAandHTSmethodsaswellaschemiluminescentdetectionofsuperoxideanionsandperoxynitriteincellsortissues.
ProductSpecifications:
Coelenterazine,native;
2-(p-Hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzyl-imidazo[1,2-a]pyrazin-3-(7H)-one
MOLECULARBIOLOGYGRADE
Formua:C26H21N3O3
MW:423.46g/mol
Purity:>95%byTLC
Storage/Handling:Storeat-20°C,andprotectfromlight.Keepunderinertgas.
Solubleinmethanolorethanol(NOTinDMSO).
Note:Itisnotrecommendedtostoredissolvedcoelenterazineat-20°Cor-70°C.Coelenterazineisahighenergydioxetanoneringstructurethatwillspontaneouslydecomposeevenatlowtemperatures.
PubChemChemicalID:2830