GoldBio/4Methylumbelliferyl phosphate, free acid/M70010/10 g

4-Methylumbelliferylphosphate(MUP)isasensitivefluorogenicsubstrateforfluorometricandUV-spectrophotometricassaysofphosphatase.

Phosphotaseactivityhaslongbeenstudiedduetoitsimportanceincellularfunction.Upondephosphorylation,MUPyieldsfluorescent4-methylumelliferone(4-MU).ThisreactionhasbeenusedtospotPCRamplifiedproductsinELISAandtoclassifybacteria.

Morespecifically,MUPcanactasanindicatorofC.perfringensbecauseofitsabilitytodetectphosphatasewhichisahighlyspecificindicatorofC.perfringens.

ProductSpecifications
4-Methylumbelliferylphosphatefreeacid
MUP

MOLECULARBIOLOGYGRADE

Formula:C₁₀H₉O₆P

MW:256.15g/mol

Color/Purity:Whitetooff-whitecrystallinepowder

PubChemChemicalID:65118

Storage/Handling:Storeat-15°Cinadry,darkplace.

Literature:
1.C.J.Cornish,etal.,Anautomatedfluorometricalkalinephosphatasemicroassaywith4-methylumbelliferylphosphateasasubstrate.Am.J.Clin.Pathol.1970,53,68.
2.H.N.Fernley,P.G.Walker,Kineticbehaviourofcalf-intestinalalkalinephosphatasewith4-methylumbelliferylphosphate.Biochem.J.1965,97,95.
3.A.Hirose,etal.,On-lineHPLCdeterminationofenzymaticactivityofalkalinephosphataseinnaturalwaterusingspectrofluorometricdetection.Chem.Lett.1993,2,307.
4.G.G.Guilbault,etal.,Umbelliferonephosphateasasubstrateforacidandalkalinephosphatase.Anal.Lett.1968,1,333.
5.R.H.Yolken,F.J.Leistner,Comparisonoffluorescentandcolorigenicsubstratesforenzymeimmunoassays.J.Clin.Microbiol.1982,15,757.