GoldBio/Taq DNA Polymerase 2x Premix with Dye/T518100/100 reactions

TaqDNAPolymeraseisathermostableDNApolymerasethatpossessesa5´→3´polymeraseactivity(1,2)anda5´flapendonucleaseactivity(3,4).TaqpolymeraseisidealforprimaryextensionreactionwithDNAfragmentshavingdAoverhangon3’ends.Thisproductissuppliedwitha10xPCRreactionbufferthatprovidesafinalconcentrationof1.5mMMg²⁺andanoptional5XGCEnhancerthatincreasestheamplificationofGCrichtemplatesupto84%.

The2xPremixwithDyecomesreadytouse,containingTaqDNAPolymerase,dNTPs,MgCl₂,stabilizersandincludesaninertbluedyeforvisibletrackingandthepreventionofpipettingmistakes,wastedtime,reagentsandmoney.IthasbeenoptimizedforroutinePCRapplications. 

ProductIncludes

• TaqDNAPolymerase2xPremixwithDye
• 5xGCEnhancer

Purity:≥98%(assessedbySDS-PAGEwithCoomassiebluestaining)

TaqConcentration(1x):0.05unit/μl
Oneunitisdefinedastheamountofenzymethatincorporates10nmolesofdNTPintoacid-insolubleformin30minutesat72ºC.

Source:E.colistrainexpressingaTaqDNAPolymerasegenefromThermusaquaticusYT-1

Storage/Handling:Storeat-20°C.
Productmaybeshippedonblueice.