PfuDNApolymeraseisaheatstableDNApolymerasewhichhas5´→3´DNApolymeraseand3´→5´exonuclease(proofreading)activities.PfuDNApolymeraseretainsthehighfidelity,sensitivityandprocessivitywithanerrorratesix-foldlowerthanTaqDNApolymerase,andsignificantlylowerthantheerrorratesofmostotherproofreadingenzymesorDNApolymerasemixtures.Thisproductissuppliedwitha10xPCRreactionbufferthatprovidesafinalconcentrationof1.5mMMg2+andanoptional5XGCEnhancerthatincreasestheamplificationofGCrichtemplatesupto84%.
ProductIncludes
Purity:≥98%(assessedbySDS-PAGEwithCoomassiebluestaining)
Concentration:5unit/μl
Oneunitisdefinedastheamountofenzymethatincorporates10nmolesofdNTPintoacid-insolubleformin30minutesat72ºC.
Source:E.colistrainexpressingaPfuDNAPolymerasegenefromPyrococcusfuriosus
Storage/Handling:Storeat-20°C.
Productmaybeshippedonblueice.