TaqDNAPolymeraseisathermostableDNApolymerasethatpossessesa5´→3´polymeraseactivity(1,2)anda5´flapendonucleaseactivity(3,4).TaqpolymeraseisidealforprimaryextensionreactionwithDNAfragmentshavingdAoverhangon3’ends.Thisproductissuppliedwitha10xPCRreactionbufferthatprovidesafinalconcentrationof1.5mMMg2+andanoptional5XGCEnhancerthatincreasestheamplificationofGCrichtemplatesupto84%.
ProductIncludes
Purity:≥98%(assessedbySDS-PAGEwithCoomassiebluestaining)
Concentration:1unit/μl
Oneunitisdefinedastheamountofenzymethatincorporates10nmolesofdNTPintoacid-insolubleformin30minutesat72ºC.
Source:E.colistrainexpressingaTaqDNAPolymerasegenefromThermusaquaticusYT-1
Storage/Handling:Storeat-20°C.
Productmaybeshippedonblueice.