L-Leucine7-amido-4-methylcoumarinhydrochlorideisusedinleucineaminopeptidaseassaysasafluorgenicsubstrate,showinggreatersensitivitywhencomparedto4-methoxynapthylamideassays.Asidefromtheleucineaminopeptidaseenzyme,L-Leucine7-amido-4-methylcoumarinhydrochloridealsoworksasafluorogenicsubtsrateforbroadspecificityaminopeptidases,suchasaminopeptidaseM.
Theresultofenzymaticactivityyieldsabrightbluefluorescentdye:7-amino-4methylcoumarin(excitation380nmemission440nm).
Leucineaminopeptidaseenzymesarefoundacrossawiderangeoforganismsincludingbacteria,plantsandanimals,andithasbeendetectedinhumantissues,withhighactivityobservedinthekidneyandliver.
ProductSpecifications
L-Leucine7-amido-4-methylcoumarinhydrochloride
L-Leucine(4-methyl-7-coumarinylamide)hydrochloride
H-Leu-AMCHCl
MOLECULARBIOLOGYGRADE
Formula:C16H20N2O3HCl
MW:324.81g/mol
Storage/Handling:Storeat-15°Cinthedark.