eBioscience Super Bright 600 染料档案

(A) Mouse splenocytes were stained with Anti-CD4 (clone RM4-5) Super Bright 600and were either analyzed immediately (red histogram), left in the dark overnight (blue histogram), or left exposed to light overnight (green histogram).

(B) Human peripheral blood cells were stained with Anti-CD8a (clone RPA-T8) Super Bright 600 and were either analyzed immediately (red histogram), left in the dark overnight (blue histogram), or left exposed to light overnight (green histogram).

4. Super Bright 436 固定后的稳定性

Stability studies indicate that Super Bright 600 exhibits a minimal loss of fluorescence when cells are exposed to various fixatives Mouse lymphocytes were stained with Anti-CD45R/B220 (clone RA3-6B2) Super Bright 600 and:

(A) were left unfixed (red histogram), or fixed in IC Fixation buffer for 30 minutes (blue histogram), 24 hours (orange histogram), or three days (green histogram), followed by a wash in Permeabilization buffer.

(B) were left unfixed (red histogram), or fixed in Foxp3/Transcription Factor/Permeabilization buffer for 30 minutes (blue histogram), 24 hours (orange histogram), or three days (green histogram), followed by a wash in Permeabilization buffer.

(C): were left unfixed (red histogram), or fixed in IC Fixation buffer followed by 90% methanol for 30 minutes (blue histogram), 24 hours (orange histogram), or three days (green histogram), followed by a Flow Cytometry Staining Buffer wash.

5. 多色分析示例- Super Bright 600.

Normal human peripheral blood cells were aliquoted in the presence of Super Bright Staining Buffer (Cat. No. SB-4400), then surface stained with the indicated reagents. Viable cells, as determined by staining with Fixable Viability Dye eFluor® 780, were used for analysis to discriminate various T cell subsets.