DNAIn® Transfection Reagent的授权代理商

DNA-In®TransfectionReagent

AUniversalTransfectionReagentforDNADelivery

DNA-In®  isthenewestgenerationtransfectionproductdevelopedbytheleadmembersofthescientificteamthatinventedtheLipofectamine®andLipofectamine®2000. DNA-In®  isanovelcationictransfectionreagentwhichbalanceshightransfectionandexpressionlevelswithlowtoxicity. DNA-In®  worksonaverybroadrangeofadherentcellsincludingmostpassagedcelllinesandmanyprimarycells.

• Productsheet

• Features

• Fast,simple,easy-to-followprotocol

• One-stepprocess:simplyaddDNA-In®  ReagenttodilutedDNA,incubate10minutes,andaddtopre-platedcells

• Minimaltoxicity-noneedtoreplacemediumaftertransfection

  DNA-In®  TransfectionReagent "Quick-StartProtocol"

  

  SuperiorTransfectionEfficiency

   

  Duringitsdevelopmentandvalidationprocess,DNA-In®  Reagentunderwentextensivetestinginmanyprimaryandculturedcells.Inahead-to-headcomparisonstudy,DNA-In®consistentlyoutperformedtheLipofectamine® 2000transfectionreagent.

  

  

  

  Cellsweretransfectedwithaplasmid,containingEmeraldGFPbehindanEF1αpromoter,witheitherDNA-In®  orLipofectamine®2000,atseveraldifferentlipid:DNAratiosandexamined~44hourspost-transfection.Representativeimagesofthebestconditionsforeachtransfectionreagentareshown.

  LowCellToxicity 

  DNA-In®  hasasignificantlylowercelltoxicitycomparedtotrADItionaltransfectionreagentssuchasLipofectamine®  andLipofectamine®  2000. Inthemanycelllinestested,DNA-In®showsabroadrangeofeffectivedosagewhilemaintainingminimalcelltoxicity. Thislowcelltoxicityallowsalargerdosageandtimewindowforoptimizedtransfectionresults.

  Thefollowingchartsshowside-by-sidecomparisonsofDNA-In®  andLipofectamine®2000(LF2K)incommonlyusedcelllinesandprimarycells:

  

  In293cells,DNA-In®  maintainshighcellviABIlitythroughoutthetesteddosagerange,whileLF2Khasasignificantcelltoxicityissuewhenusedindosagesover3µl.

  

  InA549cells,DNA-In®  hasasignificantlyhighertransfectionefficiencyandalowercelltoxicitywhileLF2Kperformspoorlyinbothparameters.

  

  Inhumanfibroblastcells,DNA-In®  hasasignificantlyhighertransfectionefficiencyandalowercelltoxicitywhileLF2Kperformspoorlyinbothparameters.

  

  InHeLacells,DNA-InhasasignificantlyhighertransfectionefficiencyandalowercelltoxicitywhileLF2Kperformspoorlyinbothparameters.

   

  

  InHepG2cells,DNA-InhasasignificantlyhighertransfectionefficiencyandalowercelltoxicitywhileLF2Kperformspoorlyinbothparameters.

  

  InMCF7cells,DNA-InhasasignificantlyhighertransfectionefficiencyandalowercelltoxicitywhileLF2Ktransfectionefficiencyislowerandistoxicwhenusedindosageshigherthan2µl.