FabGennix/PKC eta Antibody/PKCETA121AP/

GeneralInformation

Product

PKCetaAntibodyAffinityPurified

Description

AffinityPurifiedProteinkinaseCetatypeAntibodyC-epitope

VerifiedApplications

ELISA,IMM,WB

Host

Rabbit

SpeciesCrossReactivity

Human,Mouse,Rat

Immunogen

Syntheticpeptidetakenwithinaminoacidregion500-550onhumanProteinKinaseCetatypeprotein.

Accession#

NCBI: NP_006246

PhysicalProperties

Quantity

100µg

Volume

200µl

Concentration

0.55-0.75µg/µlinantibodystABIlizationbuffer

Form

AffinityPurified

Clonality

Polyclonal

Determinant

C-epitope

Storage

-20⁰Cforlongtermstorage

ApplicationProtocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:150

WesternBlot

1:500

Protein

Uniprot#

P24723

Overview

Calcium-independent,phospholipid-anddiacylglycerol(DAG)-dependentserine/threonine-proteinkinasethatisinvolvedintheregulationofcelldifferentiationinkeratinocytesandpre-Bcellreceptor,mediatesregulationofepithelialtightjunctionintegrityandfoamcellformation,andisrequiredforglioblastomaproliferationandapoptosispreventioninMCF-7cells.Inkeratinocytes,bindsandactivatesthetyrosinekinaseFYN,whichinturnblocksepidermalgrowthfactorreceptor(EGFR)signalingandleadstokeratinocytegrowtharrestanddifferentiation.AssociateswiththecyclinCCNE1-CDK2-CDKN1BcomplexandinhibitsCDK2kinaseactivity,leADIngtoRB1dephosphorylationandtherebyG1arrestinkeratinocytes.InassociationwithRALAactivatesactindepolymerization,whichisnecessaryforkeratinocytedifferentiation.Inthepre-Bcellreceptorsignaling,functionsdownstreamofBLNKbyup-regulatingIRF4,whichinturnactivatesLchaingenerearrangement.Regulatesepithelialtightjunctions(TJs)byphosphorylatingoccludin(OCLN)onthreonineresidues,whichisnecessaryfortheassemblyandmaintenanceofTJs.InassociationwithPLD2andviaTLR4signaling,isinvolvedinlipopolysaccharide(LPS)-inducedRGS2down-regulationandfoamcellformation.UponPMAstimulation,mediatesglioblastomacellproliferationbyactivatingthemTORpathway,thePI3K/AKTpathwayandtheERK1-dependentphosphorylationofELK1.Involvedintheprotectionofglioblastomacellsfromirradiation-inducedapoptosisbypreventingcaspase-9activation.Incamptothecin-treatedMCF-7cells,regulatesNF-kappa-BupstreamsignalingbyactivatingIKBKB,andconfersprotectionagainstDNAdamage-inducedapoptosis.

MolecularFunction

ATPbinding;Metalionbinding;ProteinkinaseCactivity

SubcellularLocation

Cytoplasm

Expression

Mostabundantinlung,lessinheartandskin.

Structure

TheC1domain,containingthephorbolester/DAG-typeregion1(C1A)and2(C1B),isthediacylglycerolsensorandtheC2domainisanon-calciumbindingdomain. Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.PKCsubfamily.

AlternativeNomenclature

KPCL_HUMANantibody

MGC5363antibody

MGC26269antibody

MGC5363antibody

nPKCetaantibody

PKChantibody

PKCLantibody

PRKCHantibody

PRKCLantibody

ProteinkinaseCetaantibody