产品资料

FabGennix/CD163 Antibody/CD163-112AP

Description
QA_1
Q&A

General Information
Product	CD163 AntibodyC-epitope
Verified Applications	ELISA, IF, IP, WB
Host	Rabbit
Species Cross Reactivity	Human, Monkey
Immunogen	Synthetic peptide taken within amino acid region 887-905 on human CD163 protein.
Accession #	GenBank: EAW88662.1
Physical Properties
Quantity	100 µg
Volume	200 µl
Concentration	0.55-0.75 µg/µl in antibody stabilization buffer
Form	Affinity Purified
Clonality	Polyclonal
Determinant	C-epitope
Storage	-20⁰C for long term storage
Application Protocol
DB	1:10,000
ELISA	1:10,000
Immunofluorescence	1:100
Immunoprecipitation	1:150
Western Blot	1:500
Protein
Uniprot #	D3DUU0
Overview	Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions.
Molecular Function	Scavenger receptor activity
Subcellular Location	Membrane
Expression	Expressed in monocytes and mature macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen, cortical macrophages in the thymus, resident bone marrow macrophages and meningeal macrophages of the central nervous system. Expressed also in blood. Isoform 1 is the lowest abundant in the blood. Isoform 2 is the lowest abundant in the liver and the spleen. Isoform 3 is the predominant isoform detected in the blood.
Structure	Interacts with CSNK2B.
Alternative Nomenclature	CD163 antigen antibody CD163 molecule antibody Hemoglobin Scavenger Receptor antibody Macrophage associated antigen antibody MM130 antibody Scavenger receptor cysteine rich type 1 protein M130 antibody sCD163 antibody Soluble CD163 antibody

Protein information supplied by UniProt

Hello, Was this Ab tested for Rhesus tissues? Thank you for the help.
1. There are two mismatches in the amino acid sequence, however, the epitope for this antibody appears to be in between the two mismatches, suggesting that primary antibody CD163-101AP will label Rhesus CD163. You may need to use a higher concentration of this antibody as the affinity may be slightly lower than for human or baboon CD163 protein. I would like to add that we have a second, C-epitope CD163 antibody (CD163-112AP). I blasted the sequence and found 100% homology with Rhesus protein. Feel free to let me know if you would like to samples to test both of our CD163 antibodies to test in your applications.

Hello, Was this Ab tested for Rhesus tissues? Thank you for the help.
1. There are two mismatches in the amino acid sequence, however, the epitope for this antibody appears to be in between the two mismatches, suggesting that primary antibody CD163-101AP will label Rhesus CD163. You may need to use a higher concentration of this antibody as the affinity may be slightly lower than for human or baboon CD163 protein. I would like to add that we have a second, C-epitope CD163 antibody (CD163-112AP). I blasted the sequence and found 100% homology with Rhesus protein. Feel free to let me know if you would like to samples to test both of our CD163 antibodies to test in your applications.

对照品标准品，生物试剂盒，gibco胎牛血清，试剂采购网，中国试剂网，细胞因子试剂盒

FabGennix/CD163 Antibody/CD163-112AP