FabGennix/SGK3 Antibody/SGK3301Y/

GeneralInformation

Product

SGK3Antibody

Description

AffinityPurifiedSerine/Threonine-proteinKinaseSgk3Antibody

VerifiedApplications

CM,ELISA,IC,IF,IHC,IP,WB

Host

Chicken

SpeciesCrossReactivity

Human,Mouse,Rat

Immunogen

Syntheticpeptidetakenwithinaminoacidregion440-490onhumanSgk3protein.

PhysicalProperties

Quantity

100µg

Volume

200µl

Concentration

0.6µg/µlinantibodystabilizationbuffer

Immunoglobulin

IgY

Form

AffinityPurified

Clonality

Polyclonal

Storage

-20⁰Cforlongtermstorage

ApplicationProtocol

ConfocalMicroscopy

1:100

DB

1:10,000

ELISA

1:10,000

Immunocytochemistry

1:100

Immunofluorescence

1:100

Immunohistochemistry

1:100

Immunoprecipitation

1:200

WesternBlot

1:500

Protein

Uniprot#

Q96BR1

Overview

Serine/threonine-proteinkinasewhichisinvolvedintheregulationofawidevarietyofionchannels,membranetransporters,cellgrowth,proliferation,survivalandmigration.Up-regulatesNa+channels:SCNN1A/ENACandSCN5A,K+channels:KCNA3/KV1.3,KCNE1,KCNQ1andKCNH2/HERG,epithelialCa2+channels:TRPV5andTRPV6,chloridechannel:BSND,creatinetransporter:SLC6A8,Na+/dicarboxylatecotransporter:SLC13A2/NADC1,Na+-dependentphosphatecotransporter:SLC34A2/NAPI-2B,aminoacidtransporters:SLC1A5/ASCT2andSLC6A19,glutamatetransporters:SLC1A3/EAAT1,SLC1A6/EAAT4andSLC1A7/EAAT5,glutamatereceptors:GRIA1/GLUR1andGRIK2/GLUR6,Na+/H+exchanger:SLC9A3/NHE3,andtheNa+/K+ATPase.Playsaroleintheregulationofrenaltubularphosphatetransportandbonedensity.PhosphorylatesNEDD4LandGSK3B.PositivelyregulatesERtranscriptionactivitythroughphosphorylationofFLII.NegativelyregulatesthefunctionofITCH/AIP4viaitsphosphorylationandtherebypreventsCXCR4frombeingefficientlysortedtolysosomes.

MolecularFunction

ATP-binding,Nucleotidebinding;Kinase,Serine/threonine-proteinkinase,Transferase

SubcellularLocation

Cytoplasmicvesicle;Earlyendosome;Recyclingendosome

Expression

Expressedinmosttissueswithhighestlevelsinpancreas,kidneyliver,heartandbrainandlowerlevelsinlung,placentaandskeletalmuscle.ExpressionishigherinER-positivebreasttumorsthanER-negativebreasttumors.

Structure

Belongstotheproteinkinasesuperfamily.AGCSer/Thrproteinkinasefamily.

Contains1AGC-kinaseC-terminaldomain.

Contains1proteinkinasedomain.

Contains1PX(phoxhomology)domain.

AlternativeNomenclature

CISKantibody

Cytokineindependentsurvivalkinaseantibody

DKFZp781N0293antibody

Serine/threonineproteinkinaseSgk3antibody

Serum/glucocorticoidregulatedkinasefamilymember3antibody

Serum/glucocorticoid-regulatedkinase3antibody

SGK3antibody

Sgk3antibody

SGKLantibody