FabGennix/Smad4 Antibody/SMAD4401AP/

GeneralInformation

Product

Smad4Antibody

Description

AffinityPurifiedMothersagainstdecapentaplegichomolog4AntibodyN-epitope

VerifiedApplications

ELISA,IHC,IP,WB

Host

Rabbit

SpeciesCrossReactivity

Human,Mouse,Rat

Immunogen

Syntheticpeptidetakenwithinaminoacidregion131-181onhuman SMAD4protein.

Accession#

NCBI: NP_005350.1

PhysicalProperties

Quantity

100µg

Volume

200µl

Concentration

0.6µg/µlinantibodystabilizationbuffer

Immunoglobulin

IgG

Form

AffinityPurified

Clonality

Polyclonal

Determinant

N-epitope

Storage

-20⁰Cforlongtermstorage

ApplicationProtocol

DB

1:10,000

ELISA

1:10,000

Immunoprecipitation

1:200

WesternBlot

1:500

Protein

Uniprot#

Q13485

Overview

Inmusclephysiology,playsacentralroleinbalancebetweenatrophyandhypertrophy.Mayactasatumorsuppressor.

MolecularFunction

Chromatinbinding,corepromoterproximalregionsequence-specificDNAbinding,identicalproteinbindingI-SMADbinding,metalionbinding

SubcellularLocation

Cytoplasm,nucleus(Note:Cytoplasmicintheabsenceofligand.MigratestonucleuswhencomplexedwithR-SMAD.PDPK1preventsitsnucleatranslocationinresponsetoTGF-beta.

Structure

FoundinacomplexwithSMAD1andYY1.InteractswithCITED2.Monomer;intheabsenceofTGF-betaactivation.Heterodimer;onTGF-betaactivation.ComposedoftwomoleculesofaC-terminallyphosphorylatedR-SMADmolecule,SMAD2orSMAD3,andonemoleculeofSMAD4toformthetranscriptionalactiveSMAD2/SMAD3-SMAD4complex.FoundinaternarycomplexcomposedofSMAD4,STK11/LKB1andSTK11IP.InteractswithATF2,COPS5,DACH1,MSG1,SKI,STK11/LKB1,STK11IPandTRIM33.InteractswithZNF423;theinteractiontakesplaceinresponsetoBMP2leadingtoactivationoftranscriptionofBMPtargetgenes.InteractswithZNF521;theinteractiontakesplaceinresponsetoBMP2leadingtoactivationoftranscriptionofBMPtargetgenes.InteractswithUSP9X.Interacts(viatheMH1andMH2domains)withRBPMS.InteractswithWWTR1(viacoiled-coildomain).ComponentofthemultimericcomplexSMAD3/SMAD4/JUN/FOSwhichformsattheAP1promotersite;requiredforsynergistictranscriptionalactivityinresponsetoTGF-beta.InteractswithCITED1.InteractswithPDPK1(viaPHdomain)(Bysimilarity).InteractswithVPS39;thisinteractionaffectsheterodimerformationwithSMAD3,butnotwithSMAD2,andleadstoinhibitionofSMAD3-dependenttranscriptionactivation.InteractionswithVPS39andSMAD2maybemutuallyexclusive.

AlternativeNomenclature

(Small)MothersAgainstDecapentaplegicantibodyDeletedinPancreaticCarcinoma4antibody
DeletedinPancreaticCarcinomaantibodyDPC4antibody
JIPantibody
MADmothersagainstdecapentaplegicDrosophilahomolog4antibody

MADH4antibody
Medantibody
Medeaantibody

Mothersagainstdecapentaplegic,Drosophila,homologof,4antibody

MYHRSantibody
SMA-andMAD-relatedprotein4antibody
Smad4antibody
SMADfamilymember4antibody
SMADmothersagainstDPPhomolog4antibody