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| Catalog number | HM2086 |
|---|---|
| Product type | Monoclonal antibodies |
| Quantity | 50 µg |
| Formulation | 0.5 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide. |
| Conjugate | Biotin |
| Application | Flow cytometry, Frozen sections, Functional studies, Immuno assays, Immuno fluorescence, Paraffin sections |
| Application Notes | FC: Antibody GD2.F4 stains the extracellular domain of TLR1. Monocytes were resuspended in PBS, 0.1% BSA, 0.02% NAN3 containing 15 µg/ml GD2.F4. As negative control an IgG1 isotype control was used (Ref.1). IHC-P: Tissue sections were pretreated with Target Retrieval solution, 1% Triton X-100 to improve membrane permeability and 0.03% hydrogen peroxide to quench endogenous peroxidases . Tissue sections were blocked with 2% FCS (Ref.6). IHC-F:- Tissue sections were blocked with normal horse serum prior to staining (Ref.4). FS: Antibody GD2.F4e was used to inhibit cytokine production of stimulated PBMCs (Ref.3). |
| Use | For flow cytometry and immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, dilutions have to be optimized in user’s experimental setting. |
| Immunogen | HeLa cells transfected with TLR1 mRNA (Ref.1) |
| Isotype | Mouse IgG1 |
| Species | Human |
| Alias | CD281, toll-like receptor 1, TLR1Fc |
| References | 1. Wyllie, D et al; Evidence for an accessory protein function for Toll-like receptor 1 in anti-bacterialresponses. J Immunol 2000, 165: 7125 2. Visintin, A et al; Regulation of Toll-like receptors in human monocytes and dendritic cells. JImmunol 2001, 166: 249 3. Sandor, F et al; Importance of extra- and intracellular domains of TLR1 and TLR2 in NFkBsignaling. JCB 2003, 162: 1099 4. Ochoa, M et al; Distribution of Toll-like receptor 1 and Toll-like receptor 2 in human lymphoidtissue. Immunology 2003, 108: 10 5. Ritter, M et al; Characterization of Toll-like receptors in primary lung epithelial cells: strong impactof the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins andinflammatory response. J Inflamm 2005, 2: 16 6. Månsson, A et al; A distinct Toll-like receptor repertoire in human tonsillar B cells, directlyactivated by Pam3CSK4, R-837 and CpG-2006 stimulation. Immunology 2006, 118: 539 7. Panda, A et al; Age-associated decrease in TLR function in primary human dendritic cellspredicts influenza vaccine response. J Immunol 2010, 184: 2518 |
| Storage and stability | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label. |
| Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product. |
| Disease | Infectious diseases |