Catalognumber | HM2145 |
---|---|
Producttype | Monoclonalantibodies |
Quantity | 100µg |
Formulation | 1ml(100μg/ml)0.2μmfilteredproteinGpurifiedantibodysolutioninPBS,containing0.1%bovineserum. |
Application | Flowcytometry,Functionalstudies,Immunoprecipitation,Westernblot |
ApplicationNotes | FC:AntibodyRCR-252stainstheextracellulardomainofhumanEPCR.AspositivecontrolRE-1cellswereusedandasnegativecontrolN1cells.(Ref.1) W:Anon-reducedsampletreatmentwasused.Theobservedbandsizeis50kDa(Ref.5). FS:AntibodyRCR-252functionsasblockingantbody.ItablatesAPCbindingtoEPCRandblocksAPC-mediatedsignaling.Theantibodywasfunctionallytestedbypretreatmentofcellswith10-25 µg/mlRCR-252(Ref.1-4). |
Use | Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50. |
Immunogen | HumanEPCR-positiveRE-1cells |
Isotype | RatIgG1 |
Species | Human |
Alias | ActivatedproteinCreceptor,CD201,EndothelialcellproteinCreceptor Genename:PROCR |
PositiveControl | RE-1(rat3Y1fibroblastsstablytransfectedwithhumanEPCR. |
NegativeControl | N1cells |
References | 1.Ye,Xetal;TheendothelialcellproteinCreceptor(EPCR)functionsasaprimaryreceptorforproteinCactivationonendothelialcellsinarteries,veins,andcapillaries.BiochemBiophysResCommun1999,259:671 2.Dömötör,Eetal;ActivatedproteinCalterscytosoliccalciumfluxinhumanbrainendotheliumviabindingtoendothelialproteinCreceptorandactivationofproteaseactivatedreceptor-1.Blood2003,101:4797 3.Sturn,Detal;ExpressionandfunctionoftheendothelialproteinCreceptorinhumanneutrophils.Blood2003,102:1499 4.Finigan,Jetal;ActivatedproteinCmediatesnovellungendothelialbarriereenhancement.JBiolChem2005,280:17286 5.BaeJetal;ReceptorsoftheproteinCactivationandactivatedproteinCsignalingpathwaysarecolocalizedinlipidraftsofendothelialcells.PNAS2006,104:2867 |
Storageandstability | Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear. |
Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
Disease | Cardiologyandmetabolism |
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