产品资料

Hycult Biotech/EPCR, Human, mAb RCR-252/HM2145/100 µg

Description:EPCR,Human,mAbRCR-252

ThemonoclonalantibodyRCR-252recognizeshumanendothelialproteinCreceptor(EPCR),ahighlyglycosylatedtypeItransmembraneproteinof221-amino-acids.Theseaminoacidscompriseanextracellulardomain,a25-aatransmembranedomain,andashort(3aa)intracytoplasmicsequencecodingforan~46kDaprotein.Deglycosylationwillreducetheproteinmassto25kDa.EPCRisexpressedstronglyontheendothelialcellsofarteriesandveinsinheartandlung,lessintenselyincapillariesinthelungandskin,andnotatallintheendotheliumofsmallvesselsoftheliverandkidney.
EPCRisthereceptorforproteinC,akeyplayerintheanticoagulationpathway.TheproteinCanticoagulantpathwayservesasamajorsystemforcontrolingthrombosis,limitinginflammatoryresponses,andpotentiallydecreasingendothelialcellapoptosisinresponsetoinflammatorycytokinesandischemia.Theessentialcomponentsofthepathwayincludethrombin,thrombomodulin,theendothelialcellproteinCreceptor(EPCR),proteinCandproteinS.Thepathwayisinitiatedwhenthrombinbindstothrombomodulinonthesurfaceofendothelium.EPCRaugmentsproteinCactivationbybindingproteinCandpresentingittothethrombin-thrombomodulinactivationcomplex.ActivatedproteinC(aPC)retainsitsABIlitytobindEPCR,andthiscomplexappearstobeinvolvedinsomeofthecellularsignalingmechanismsthatdown-regulateinflammatorycytokineformation(TNF,IL-6).EPCRisshedfromthevasculaturebyinflammatorymediatorsandthrombin.EPCRbindstoactivatedneutrophilsinaprocessthatinvolvesproteinase3andMac-1.FurThermore,EPCRcanundergotranslocationfromtheplasmamembranetothenucleus.
EPCRcanbecleavedtoreleaseasolubleform(sEPCR)inthecirculation.ThissEPCRisdetectedasasinglespeciesof43kDa,resultingfromsheddingofmembraneEPCRbytheactionofametalloprotease,-whichisstimulatedbythrombinandbysomeinflammatorymediators.SolubleEPCRbindsPCandaPCwithsimilaraffinity,butitsbindingtoaPCinhibitstheanticoagulantactivityofaPCbyblockingitsbindingtophospholipidsandbyabrogatingitsabilitytoinactivatefactorVa.sEPCR-canbedetectedinplasma.Innormalpersons,sEPCRispresentinlevelsof83.6+/-17.2ng/ml.ElevatedlevelsofsEPCRarepositivelycorrelatedtoahigherriskforthrombosis.Furthermore,ahaplotype(A3allele)hasbeenlinkedtoelevatedlevelsofsEPCR(264+/-174ng/ml).

Specifications:

Catalognumber	HM2145
Producttype	Monoclonalantibodies
Quantity	100µg
Formulation	1ml(100μg/ml)0.2μmfilteredproteinGpurifiedantibodysolutioninPBS,containing0.1%bovineserum.
Application	Flowcytometry,Functionalstudies,Immunoprecipitation,Westernblot
ApplicationNotes	FC:AntibodyRCR-252stainstheextracellulardomainofhumanEPCR.AspositivecontrolRE-1cellswereusedandasnegativecontrolN1cells.(Ref.1) W:Anon-reducedsampletreatmentwasused.Theobservedbandsizeis50kDa(Ref.5). FS:AntibodyRCR-252functionsasblockingantbody.ItablatesAPCbindingtoEPCRandblocksAPC-mediatedsignaling.Theantibodywasfunctionallytestedbypretreatmentofcellswith10-25 µg/mlRCR-252(Ref.1-4).
Use	Dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.
Immunogen	HumanEPCR-positiveRE-1cells
Isotype	RatIgG1
Species	Human
Alias	ActivatedproteinCreceptor,CD201,EndothelialcellproteinCreceptor Genename:PROCR
PositiveControl	RE-1(rat3Y1fibroblastsstablytransfectedwithhumanEPCR.
NegativeControl	N1cells
References	1.Ye,Xetal;TheendothelialcellproteinCreceptor(EPCR)functionsasaprimaryreceptorforproteinCactivationonendothelialcellsinarteries,veins,andcapillaries.BiochemBiophysResCommun1999,259:671 2.Dömötör,Eetal;ActivatedproteinCalterscytosoliccalciumfluxinhumanbrainendotheliumviabindingtoendothelialproteinCreceptorandactivationofproteaseactivatedreceptor-1.Blood2003,101:4797 3.Sturn,Detal;ExpressionandfunctionoftheendothelialproteinCreceptorinhumanneutrophils.Blood2003,102:1499 4.Finigan,Jetal;ActivatedproteinCmediatesnovellungendothelialbarriereenhancement.JBiolChem2005,280:17286 5.BaeJetal;ReceptorsoftheproteinCactivationandactivatedproteinCsignalingpathwaysarecolocalizedinlipidraftsofendothelialcells.PNAS2006,104:2867
Storageandstability	Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.
Precautions	Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.
Disease	Cardiologyandmetabolism

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Hycult Biotech/EPCR, Human, mAb RCR-252/HM2145/100 µg

Description:EPCR,Human,mAbRCR-252

Specifications: