产品资料

Hycult Biotech/C3/C3a, Human, mAb 2898, immuno assay antibody/HM2075-IA/0.5 mg

Description:C3/C3a,Human,mAb2898,immunoassayantibody

Themonoclonalantibody2898recognizestheC-terminusofhumancomplementproteinC3a.TheantibodyrecognizesintactC3,C3aaswellasC3a-desArg.C3isthemostabundantproteinofthecomplementsystemwithserumproteinlevelsofabout1.3mg/ml.Thecomplementsystemisanimportantpartofthehumoralresponseininnateimmunity,consistingofthreedifferentpathways.Thethirdcomplementcomponent,C3,iscentraltotheclassical,alternativeandlectinpathwaysofcomplementactivation.Activationproductsofthecomplementcascadecontainneo-epitopesthatarenotpresentintheindividualnativecomponents.Monoclonalantibodiesdetectingneo-epitopeshavebeenusedfordirectquantificationofactivationatdifferentstepsinthecomplementcascade.ThesynthesisofC3istissue-specificandismodulatedinresponsetoavarietyofstimulatoryagents.AninheriteddeficiencyofC3predisposesthepersontofrequentassaultsofbacterialinfections.Inulcerativecolitis,andidiopathicchronicinflammatoryboweldisease,thedepositionofC3inthediseasedmucosahasbeenreported.ProteolysisbycertainenzymesresultsinthecleavageofC3releasingC3aanaphylatoxinandC3b.C3aisaproteinof74aminoacids.C3aitselfisveryshort-livedandinserumiscleavedrapidlyintothemorestableC3a-desArg(alsocalledacylationstimulatingprotein,ASP).Therefore,measurementofC3a-desArgallowsreliableconclusionsaboutthelevelofcomplementactivationinthetestsamples.
C3aisamediatoroflocalinflammatoryprocesses.Itinducessmoothmusclecontraction,increasesvascularpermeABIlity,andcauseshistaminereleasefrommastcellsandbasophilicleukocytes.C3aisinvolvedininflammatoryreactionsseeningram-negativebacterialsepsis,trauma,ischemicheartdisease,post-dialysissyndromeandavarietyofautoimmunediseases.

Specifications:

Catalognumber	HM2075-IA
Producttype	Monoclonalantibodies
Quantity	0.5mg
Formulation	0.5mgof0.2µmfilteredantibodysolutioninPBS(exactconcentrationisindicatedonthelabel).
Application	Immunoassays,Westernblot
ApplicationNotes	W:AreducedsampletreatmentandSDS-PAGEwasused.Thebandsizeis~95and8.2kDaforC3andC3arespectively(Ref.1).
Use	ForWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.
Immunogen	PeptideCARASHLGLA(lastnineaminoacidsofC3a-desArg)
Isotype	MouseIgG1
Species	Human
Alias	ComplementproteinC3
PositiveControl	Humanplasma
References	1.Hartmann,Hetal;RapidquantificationofC3aandC5ausingacombinationofchromatographicandimmunoassayprocedures.JImmunolMeth1993,166:35 2.Smalley,Detal;Proteomicdiscoveryof21proteinsexpressedinhumanplasma-derivedbutnotplatelet-derivedmicroparticles.ThrombHaemost2007,97:67
Storageandstability	Productshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel.
Precautions	Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct.
Disease	Infectiousdiseases,Nephrology

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Hycult Biotech/C3/C3a, Human, mAb 2898, immuno assay antibody/HM2075-IA/0.5 mg

Description:C3/C3a,Human,mAb2898,immunoassayantibody

Specifications: