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| Catalog number | HM1022 |
|---|---|
| Product type | Monoclonal antibodies |
| Quantity | 100 µg |
| Formulation | 1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin. |
| Application | Functional studies, Immuno assays, Immuno precipitation, Western blot |
| Application Notes | The antibody can be used for immuno assays, Western blotting, immuno precipitation and purification. Furthermore the antibody is useful for inhibition in biological assays, in vitro as well as in vivo. |
| Use | For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts IL-1RI to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions. |
| Isotype | Rat IgG1 |
| Species | Mouse |
| References | 1. Kojouharoff, G et al; Neutralization of tumour necrosis factor (TNF) but not of IL-1 reduces inflammation in chronic dextran sulphate sodium induced colitis in mice. Clin Exp Immunol 1997, 107: 353 2. Stern, A et al; Purification to homogeneity and amino acid sequence analysis of a receptor protein for interleu- kin-1. Arch Biochem Biophys 1989, 274: 26 |
| Storage and stability | Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year. |
| Precautions | For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and Federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result with the use of or derivation of this product. |
| Disease | Autoimmunity, Neurological disorders |