SchwannCellMedium
(SCM)
CatalogNumber:1701
ProductDescriptionSchwanncellmedium(SCM)isacompletemediumdesignedforoptimalgrowthofnormalhumanSchwanncellsinvitro.Itisasterile,liquidmediumwhichcontainsessentialandnon-essentialaminoacids,vitamins,organicandinorganiccompounds,hormones,growthfactors,tracemineralsandalowconcentrationoffetalbovineserum(5%).ThemediumisbicarbonatebufferedandhasapHof7.4whenequilibratedinanincubatorwithanatmosphereof5%CO2/95%air.Themediumisformulated(quantitativelyandqualitatively)toprovideadefinedandoptimallybalancednutritionalenvironmentthatselectivelypromotesgrowthofnormalhumanastrocytesinvitro.
ComponentsSCMconsistsof500mlofbasalmedium,25mloffetalbovineserum(FBS,Cat.No.0025),5mlofSchwanncellgrowthsupplement(SCGS,Cat.No.1752)and5mlofpenicillin/streptomycinsolution(P/S,Cat.No.0503).
ProductUseSCMisforresearchuseonly.Itisnotapprovedforhumanoranimaluse,orforapplicationininvitrodiagnosticprocedures.
Storage
Storethebasalmediumat4oC,theSCGS,theFBSandtheP/Ssolutionat-20oC.Protectfromlight.
ShippingGelice.
PrepareforuseThawSCGS,FBSandP/Ssolutionat37oC.GentlytilttheSCGStubeseveraltimesduringthawingtohelpthecontentsdissolve.Makesurethecontentsofthesupplementarecompletelydissolvedintosolutionbeforeaddingtothemedium.Rinsethebottleandtubeswith70%ethanol,andthenwipetoremoveexcess.Removethecap,beingcarefulnottotouchtheinteriorthreadswithfingers.AddSCGS,FBSandP/Ssolutionintobasalmediuminasterilefield,mixwellandthenthereconstitutedmediumisreadyforuse.Sinceseveralcomponentsofthismediumarelight-l
ABIle,itisrecommendedthatthemediumnotbeexposedtolightforlengthyperiodsoftime.Ifthemediumiswarmedpriortouse,donotexceed37oC.Whenstoredinthedarkat4oC,thereconstitutedmediumisstableforonemonth.
Caution:Ifhandledimproperly,somecomponentsofthemediummaypresentahealthhazard.Takeappropriateprecautionswhenhandlingit,includingthewearingofprotectiveclothingandeyewear.Disposeofproperly.http://www.yuhengfengbio.com/display01.asp