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Signosis/HOXA-5 EMSA Kit/GS-0061/1 Ea

Description:

WhenoneTFisactivated,itbindstoabiotin-labeledprobe,whichisdesignedbasedontheconsensusbindingsequenceoftheTF.Whentheformedcomplexissubjectedtoelectrophoresis,itrunsslowerthanthefreeprobe,andcanbeeasilydistinguished.Twoormoresamplescanbecomparedanddifferencescanbedeterminedbythedensityoftheshiftedband.


Principle:

WhenoneTFisactivated,itbindstoabiotin-labeledprobe,whichisdesignedbasedontheconsensusbindingsequenceoftheTF.Whentheformedcomplexissubjectedtoelectrophoresis,itrunsslowerthanthefreeprobe,andcanbeeasilydistinguished.Twoormoresamplescanbecomparedanddifferencescanbedeterminedbythedensityoftheshiftedband.


Data:

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