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| Note: Theproductisforresearchuseonly,notforuseindiagnosticortherapeuticprocedures | |
| ProductName | POLR2A(Phospho-Ser1619)Antibody |
| Synonyms | DNA-directedRNApolymeraseIIlargestsubunit;RNApolIIlargestsubunit;RNApolymeraseII220kdsubunit;RPB1 |
| Immunogen | TheantiserumwasproducedagainstsynthesizedpeptidederivedfromhumanPOLR2AaroundthephosphorylationsiteofSer1619. |
| ImmunogenRange | 1585-1634 |
| Clonality | Polyclonal |
| ApplicationDilutions | WB:1:500~1:1000IHC:1:50~1:100IF:1:100~1:500ELISA:1:10000 |
| Specificity | POLR2A(Phospho-Ser1619)AntibodydetectsendogenouslevelsofPOLR2AonlywhenphosphorylatedatSer1619. |
| Purification | Theantibodywaspurifiedfromrabbitantiserumbyaffinity-chromatographyusingphosphopeptide.Theantibodyagainstnon-phosphopeptidewasremovedbychromatographyusingcorrespondingnon-phosphopeptide. |
| PhysicalForm | RabbitIgGinphosphatebufferedsaline(withoutMg2+andCa2+),pH7.4,150mMNaCl,0.02%sodiumazideand50%glycerol. |
| Storage/StABIlity | Stableat-20°Cforatleast1year. |
| NCBIGeneSymbol | RPB1 |
| DatabaseLinks | GeneID: 5430 Swiss-Prot#: P24928 OMIM#: 180660 Unigene#: Hs.270017 |
| TargetModification | Phospho |
| ModificationSites | H:S1619M:S1619R:S1619 |
| ApplicationImages | |
| Image1 |  ImmunofluorescenceanalysisofHeLacellstreatedwithPMA125ng/ml30",usingPOLR2A(Phospho-Ser1619)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image2 |  Immunohistochemistryanalysisofparaffin-embeddedhumanbreastcarcinoma,usingPOLR2A(Phospho-Ser1619)Antibody.Thepictureontherightisblockedwiththephosphopeptide. |
| Image3 |  WesternblotanalysisoflysatesfromCOS7cellstreatedwithEGF200ng/ml30",usingPOLR2A(Phospho-Ser1619)Antibody.Thelaneontherightisblockedwiththephosphopeptide. |
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