31106300,RevMAb Biosciences,Rabbit Monoclonal,RevMAb Biosciences,+,兔单克隆,Array.Array.Array蚂蚁淘厂家直采.

Rabbit MonoclonalRevmab/Anti-Acetyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM149; H3K4ac, Histone H3 (K4 acetylation)/31-1063-00/
RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-...
ProductDetailsProductNameAnti-Acetyl-HistoneH3(Lys4)RabbitMonoclonalAntibody[RM149]ProductDescriptionRabbitmonoclonaltoAcetylatedHistoneH3Lysine4(K4ac),H3K4ac,HistoneH3(K4acetylation)CatalogNo.31-1063-00CloneNameRM149ProductDataSheetAnti-Acetyl-HistoneH3Lys4RabbitMonoclonalAntibodyDataSheetRM149SpecificityRM149reactstoHistoneH3acetylatedatLysine4(K4ac).Nocrossreactivitywithnon-modifiedLysine4orotheracetylatedLysinesinhistoneH3.ImmunogenAnacetyl-peptidecorrespondingtoAcetyl-HistoneH3(Lys4).ApplicationWesternBlot(WB)
Immunocytochemistry(ICC)
ELISA
Multiplex
SpeciesReactivityHuman
VertebratesConjugateNoneIntendedUseResearchUseOnly.PropertiesFormLiquidStorageInstructionsStoreat-20.0°CStABIlityStablefor1Yearat-20.0°CfromdateofreceiptStorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazideVolume/Size100ugConcentration1.0mg/mLPurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatantCloneTypeMonoclonalIsotypeRabbitIgGUsageWesternBlot:0.25ug/mL-1ug/mL;
ICC:0.5ug/mL-2ug/mL;
ELISA:0.2ug/mL-1ug/mL;
Multiplex:0.05ug/mL–0.5ug/mL.

Keywords.ProductImages

RM149specificallyreactstoHistoneH3acetylated
at Lysine 4 (K4ac). No cross reactivity with non-
modified Lysine 4, acetylated Lysine 9 (K9ac), Lysine
14 (K14ac), Lysine 18 (K18ac),Lysine 23 (K23ac),
Lysine 27 (K27ac), Lysine 36 (K36ac), Lysine 56
(K56ac), lysine 79 (K79ac), or Lysine 122 (K122) in
histone H3.

WesternBlotofacidextractsfromHeLacells
untreated (-) or treated with sodium butyrate (+),
using RM149 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 4 in treated HeLa.

ImmunocytochemistryofHeLacellstreatedwith
sodium butyrate, using Acetyl-Histone H3 (Lys4)
Rabbit mAb RM149 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.