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Revmab/Anti-Histone H3 K27M Rabbit Monoclonal Antibody, Clone RM192; Histone H3, K27M Mutant (50ug)/31-1175-00/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Revmab/Anti-Histone H3 K27M Rabbit Monoclonal Antibody, Clone RM192; Histone H3, K27M Mutant (50ug)/31-1175-00/1 Ea

ProductDetails
ProductNameAnti-HistoneH3K27MRabbitMonoclonalAntibody[RM192]
ProductDescriptionRabbitmonoclonaltoHistoneH3K27M;HistoneH3,K27MMutant
CatalogNo.31-1175-00
CloneNameRM192
ProductDataSheetAnti-HistoneH3K27MRabbitMonoclonalAntibodyDataSheetRM192
SpecificityRM192reactstotheHistoneH3K27Mmutant.NocrossreactivitywithwildtypeHistoneH3.
ImmunogenApeptidecorrespondingtoHistoneH3K27Mmutant
ApplicationWesternBlot
ELISA
Immunohistochemistry
Immunocytochemistry
SpeciesReactivityAll
ConjugateNone
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/Size50ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageWesternBlot:0.01ug/mL–1ug/mL;
ELISA:0.5ug/mL-2ug/mL;
Immunohistochemistry(IHC):0.005–0.1ug/mL;
Immunocytochemistry(ICC/IF):0.005–0.1ug/mL.
ReferencesforHistoneH3K27MMutantantibody[RM192]Pathaniaetal.H3.3K27MCooperateswithTrp53LossandPDGFRAGaininMouseEmbryonicNeuralProgenitor
CellstoInduceInvasiveHigh-GradeGliomasCancerCell(2017)10.1016/j.ccell.2017.09.014.IHC-P;Human.Readmore(PubMed:29107533)
.
ProductImages

Immunostainingofbraintumortissuesections
using both EDTA and Citrate pretreatment methods.
Clinically validated to be Positive for the H3.3 K27M
Mutation by Sanger sequencing.


ImagecourtesyofSebastianBrandnerMD,Division
of Neuropathology and Dept. of Neurodegenerative Disease,
UCL Institute of Neurology, London, UK

Immunostainingofbraintumortissuesections
using both EDTA and Citrate pretreatment methods.
Clinically validated to be Negative for the H3.3 K27M
Mutation by Sanger sequencing.


ImagecourtesyofSebastianBrandnerMD,Division
of Neuropathology and Dept. of Neurodegenerative Disease,
UCL Institute of Neurology, London, UK

Western

WesternBlotanalysisofcelllysatespreparedfrom
293T transfected with a DNA construct encoding
wild type (WT) or K27M mutant proteins of Histone
H3.3, using 0.01 ug/mL of anti-Histone H3 K27M
rabbit monoclonal antibody clone RM192.

Immunohistochemical

Immunohistochemicalstainingofformalinfixed
and paraffin embedded 293T cells transfected
with a DNA construct encoding Histone H3 K27M
mutant, stained with 0.01 ug/mL of anti-Histone H3
K27M rabbit monoclonal antibody clone RM192.

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