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Revmab/Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM103/31-1007-00/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Revmab/Anti-Mouse Immunoglobulin Kappa Light Chain Rabbit Monoclonal Antibody, Clone RM103/31-1007-00/1 Ea

ProductDetails
ProductNameAnti-MouseImmunoglobulinKappaLightChainRabbitMonoclonalAntibody[RM103]
ProductDescriptionRabbitmonoclonaltoMouseIgKappaLightChain
CatalogNo.31-1007-00
CloneNameRM103
ProductDataSheetAnti-MouseIgKappaRabbitMonoclonalAntibodyDataSheetRM103
SpecificityThisantibodyreactstothekappalightchainofmouseimmunoglobulins.Nocrossreactivitywiththelamdalightchain,humanIgG,ratIgG,orgoatIgG.
TheFcregionofRM103hasbeenengineeredtoeliminateFcreceptorbinding.
ImmunogenMouseIgG
ApplicationWesternBlot(WB)(nonreduced)
Immunoprecipitation(IP)
Immunohistochemistry(IHC)
FlowCytometry(FC)
Immunocytochemistry(ICC)
ELISA
SpeciesReactivityMouse
ConjugateNone
IntendedUseResearchUseOnly
.
Properties
FormLiquid
StorageInstructionsStoreat-20.0°C
StABIlityStablefor1Yearat-20.0°Cfromdateofreceipt
StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazide
Volume/ItemSize100ug
Concentration1.0mg/mL
PurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatant
CloneTypeMonoclonal
IsotypeRabbitIgG
UsageELISA:0.005ug/mL-0.2ug/mL;
Immunocytochemistry(ICC):0.5ug/mL-2ug/mL;
Immunohistochemistry(IHC):0.5ug/mL-2ug/mL;
WesternBlot(WB):0.1ug/mL-0.5ug/mL.
Keywords
.
ProductImages
Western

Westernblotofnonreduced(-)andreduced(+)
mouse IgG1κ and IgG1λ (20ng/lane), using 0.2ug/mL
of RevmabcloneRM103.Thisantibodyreactsto
 nonreduced IgG1κ (~150 kDa), and slightly reacts
 to reduced κ light chain (~25 kDa).

ELISA

ELISAofmouseimmunoglobulinsshowsRM103
reacts to the kappa light chain of mouse immuno-
globulins. No cross reactivity with the lamda light
chain, human IgG (κ+λ), rat IgG (κ+λ), or goat IgG
(κ+λ). The plate was coated with 50 ng/well of
different immunoglobulins. 200 ng/mL, 50 ng/mL,
or 10 ng/mL of RM103 was used as the primary
antibody. An alkaline phosphatase conjugated
 anti-rabbit IgG as the secondary antibody.

A

AtiterELISAofmouseIgG1κ.Theplatewascoated
with different amounts of mouse IgG1κ. A serial
 dilution of RM103 was used as the primary antibody.
An alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.

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