31106400,RevMAb Biosciences,Rabbit Monoclonal,RevMAb Biosciences,+,兔单克隆,Array.Array.Array蚂蚁淘厂家直采.

Rabbit MonoclonalRevmab/Anti-Monomethyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody, Clone RM150; H3K9me1, Histone H3 (monomethyl K9)/31-1064
RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-...
ProductDetailsProductNameAnti-Monomethyl-HistoneH3(Lys9)RabbitMonoclonalAntibody[RM150]ProductDescriptionRabbitmonoclonaltoMonomethylatedHistoneH3Lysine9,H3K9me1,HistoneH3(monomethylK9)CatalogNo.31-1064-00CloneNameRM150ProductDataSheetAnti-Monomethyl-HistoneH3(Lys9)RabbitMonoclonalAntibodyDataSheetRM150SpecificityRM150reactstoHistoneH3monomethylatedatLysine9(K9me1).Nocrossreactivitywithnon-modifiedLysine9,dimethylatedLysine9(K9me2),trimethylatedLysine4(K9me3),orothermethylationsinhistoneH3.ImmunogenAmonomethyl-peptidecorrespondingtoMonomethyl-HistoneH3(Lys9).ApplicationWesternBlot(WB)
ChromatinIP(ChIP)
Immunocytochemistry(ICC)
ELISA
Multiplex
SpeciesReactivityHuman
VertebratesConjugateNoneIntendedUseResearchUseOnly.PropertiesFormLiquidStorageInstructionsStoreat-20.0°CStABIlityStablefor1Yearat-20.0°Cfromdateofreceipt.StorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazideVolume/Size100ugConcentration1.0mg/mLPurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatantCloneTypeMonoclonalIsotypeRabbitIgGUsageWesternBlot:0.2ug/mL-1ug/mL;
ChIP:2ug/mL-10ug/mL
ICC:0.5ug/mL-2ug/mL;
ELISA:0.2ug/mL-1ug/mL;
Multiplex:0.1ug/mL–0.5ug/mL.



Keywords.ProductImages

RM150specificallyreactstoHistoneH3monomethy-
lated at Lysine 9 (K9me1). No cross reactivity with non-
modified Lysine 9, dimethylated Lysine 9 (K9me2),
trimethylated Lysine 4 (K9me3), or other methylations
in histone H3.

WesternBlotofrecombinanthistoneH3.3(1)and
acid extracts of HeLa cells (2), using RM150 at 0.5
ug/mL, showed a band of histone H3 monomethy-
lated at Lysine 9 (K9me1) in HeLa cells.

ChIPperformedonHeLacellsusingH3K9me1antibody
(RM150, 5ug). Real-time PCR was performed using
primers specific to the gene indicated.

ImmunocytochemistryofHeLacellstreatedwith
sodium butyrate, using Monomethyl-Histone H3
(Lys9) Rabbit mAb RM150 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.