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| Catalognumber | HK501-01 |
|---|---|
| Producttype | Assays |
| Quantity | 1x96det. |
| Standardrange | 2-1,500nM |
| Detectionlevel | 2nM |
| Workingvolume | 100µl/well |
| Species | N/A |
| Application | TheNitrotyrosineELISAkitistobeusedfortheinvitroquantitativedeterminationofnitrotyrosineinplasmaandotherbiologicalsamples. |
| Disease | Autoimmunity,Cardiologyandmetabolism |
| Principle | TheNitrotyrosineELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof3½hours.Theefficientformatofaplatewithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.Samplesandstandardsareincubatedinmicrotiterwellscoatedwithantibodiesrecognizingnitrotyrosine.Biotinylatedtracerantibodywillbindtocapturednitrotyrosine.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofcitricacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthenitrotyrosinestandards(log).Thenitrotyrosineconcentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve. |
| Storageandstability | Productshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear. |
| Precautions | Forresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct. |
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