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基底材料的拓扑形貌是影响细胞行为的重要因素之一,细胞与微纳米结构图案表面相互作用研究在细胞生物学、再生医学和组织工程领域具有重要的意义。材料表面微纳米图案化不但可以提供结构模版,用以研究细胞对生长环境的响应特性,而且可以为组织再生用支架和植入性器件的设计提供基础数据。
实验结果显示,细胞会根据材料基底表面的图案结构产生相应的形变,而且这种形变响应与图案几何形状、尺寸,细胞伸展和基底表面的黏附蛋白浓度有关。在一定范围内,图案越大、越深,细胞形变越大；细胞伸展面积越大,形变越小：黏附蛋白可以促进细胞伸展,从而使细胞形变减小.微米坑图案能够明显促进细胞在材料表面的黏附；微米沟槽图案则对细胞具有接触诱导效应,可以诱导细胞响应所生长的微纳米沟槽结构进行骨架重排,从而引起细胞取向行为变化.
CYTOO品牌的细胞微图案产品包括CYTOOPLATE?细胞微图案微孔培养板和CYTOOChips细胞微图案芯片:CYTOOPLATE?细胞微图案微孔培养板是一个高清晰度的低荧光硼硅玻璃底细胞光刻微图案，CYTOOChips细胞微图案芯片可以控制细胞粘附性状、大小、细胞培养分化等，研究细胞取，这种方法可以得到稳定的具有显著细胞黏附反差的"微图案化表面.利用该图案化表面技术引导细胞黏附后的取向，先在细胞无法贴附的Cytophobic涂料处理，再以光蚀刻"的技术，精准地留下特定的几何形狀，并在这样的几形状上涂上特定的细胞间质蛋白(extracellular"matrix,ECM,suchasfibronecin,collgene,Laminin,ect…)。这样的细胞培养工具借由精准的控制细胞贴附的型式，提供了体外培养细胞结构上的引导，使细胞表现出更接近体内生理的表型及框架，这样的工具可以:
◆提高试验的再现性，灵敏性和归纳量化复杂的生物性狀
◆进一步分析细胞结构机械性刺激和细胞功能之间的关系
◆模拟重现细胞在生物体内的环境，并使性狀易于观察分析
"CYTOO细胞微图案(CYTOOmicropattern)使体外培养细胞重现体内应有的细胞极性，有组织的整齐排列，细胞移动的限制!
量化活细胞分析的突破
精准的对照条件在CYTOO阵列上，细胞呈现相同的结构
标准的培养皿中的细胞CYTOO培养产品中的细胞
"细胞贴附在涂有细胞间质蛋白的微图案(micropattern)阵列上时，会随着微图案（micropattern）的引导在不能贴附的材质上张开成特定的几何结构。这样特定形状的贴附接触分布，促使细胞产生固定且再现性极高的极性。细胞位置、细胞形状、细胞极性、以及胞器位置都可标准化.
重现体内细胞的物理环境
使体外培养细胞的生理性狀更接近体内组织
CYTOOchipTM细胞微图案芯片
19.5x19.5mm盖玻片，由光刻法在高质低荧光硼硅酸盐玻璃上刻画微图形，用于倒置显微镜，"网状结构打印在芯片底部，使用户可以随时找到所需细胞。这些芯片与CYTOOchamber芯片槽结合可实现高分辨率活细胞成像。
标准芯片和平板可用于所有一般用途，初始包的设计是用来开发微图形形状和尺寸的，Arena芯片分为不同尺寸的磁盘，客户定制产品可以使您达到zui优实验方案。
CYTOOchips细胞微图案芯片客户定制产品包括19.5x19.5mm盖玻片，客户可定制微图形，且粘附蛋白可适用于多种应用和实验。定制CYTOOchipszui小量为1组18个芯片，且包裹纤连蛋白或活性表面。
客户定制范围包括：
产品规格
产品名称
货号
(PATTERN)图案
SIZE规格
COATING涂层
CYTOOCHIPS?MOTILITYAX18
10-031-00-18
Multiple
Multiple
Activated
CYTOOCHIPS?ARENAAX18
10-020-00-18
Disc
Multiple
Activated
CYTOOCHIPS?CUSTOMAX18
10-950-00-18
Multiple
Custom
Activated
CYTOOCHIPS?L-L-AX18
10-015-00-18
L
Large(1600μm2)
Activated
CYTOOCHIPS?L-S-AX18
10-013-00-18
L
Small(700μm2)
Activated
CYTOOCHIPS?L-M-AX18
10-014-00-18
L
Medium(1100μm2)
Activated
CYTOOCHIPS?STARTER"SAX18
10-900-00-18
Multiple
Multiple
Activated
CYTOOCHIPS?DC-S-AX18
10-001-00-18
Disc
Small(700μm2)
Activated
CYTOOCHIPS?DC-M-AX18
10-002-00-18
Disc
Medium(1100μm2)
Activated
CYTOOCHIPS?DC-L-AX18
10-003-00-18
Disc
Large(1600μm2)
Activated
CYTOOCHIPS?CW-L-AX18
10-006-00-18
Crossbow
Large(1600μm2)
Activated
CYTOOCHIPS?CW-S-AX18
10-004-00-18
Crossbow
Small(700μm2)
Activated
CYTOOCHIPS?CW-M-AX18
10-005-00-18
Crossbow
Medium(1100μm2)
Activated
CYTOOCHIPS?Y-L-AX18
10-012-00-18
Y
Large(1600μm2)
Activated
CYTOOCHIPS?Y-M-AX18
10-011-00-18
Y
Medium(1100μm2)
Activated
CYTOOCHIPS?Y-S-AX18
10-010-00-18
Y
Small(700μm2)
Activated
CYTOOCHIPS?H-L-AX18
10-009-00-18
H
Large(1600μm2)
Activated
CYTOOCHIPS?H-M-AX18
10-008-00-18
H
Medium(1100μm2)
Activated
CYTOOCHIPS?H-S-AX18
10-007-00-18
H
Small(700μm2)
Activated
CYTOOplates细胞微图案培养板
CYTOOplates平板为标准SBS微平板版式，由光刻法在高质低荧光硼硅酸盐玻璃底部刻画微图形，可适用于高内涵筛选，且可适用于所有普通HCS成像平台和实验室自动化仪器。
"CYTOOplates客户定制产品包括96孔标准SBS版式，客户可定制微图形，且粘附蛋白可适用于多种应用和实验，"无论所需的微图形形状、尺寸或96孔布局如何。定制zui小量为10个平板，且包裹纤连蛋白或活性表面。
客户定制范围包括:
产品名称
货号
(PATTERN)图案
SIZE规格
COATING涂层
CYTOOPLATES?96RWL-S-A
20-013-00
L
Small(700μm2)
Activated
CYTOOPLATES?96RWL-L-A
20-015-00
L
Large(1600μm2)
Activated
CYTOOPLATES?96RWL-M-A
20-015-00
L
Medium(1100μm2)
Activated
CYTOOPLATES?96RWDC-S-A
20-001-00
Disc
Small(700μm2)
Activated
CYTOOPLATES?96RWDC-M-A
20-002-00
Disc
Medium(1100μm2)
Activated
CYTOOPLATES?96RWDC-L-A
20-003-00
Disc
Large(1600μm2)
Activated
CYTOOPLATES?96RWCW-S-A
20-004-00
Crossbow
Small(700μm2)
Activated
CYTOOPLATES?96RWCW-M-A
20-005-00
Crossbow
Medium(1100μm2)
Activated
CYTOOPLATES?96RWCW-L-A
20-006-00
Crossbow
Large(1600μm2)
Activated
CYTOOPLATES?96RWH-S-A
20-007-00
H
Small(700μm2)
Activated
CYTOOPLATES?96RWH-M-A
20-008-00
H
Medium(1100μm2)
Activated
CYTOOPLATES?96RWH-L-A
20-009-00
H
Large(1600μm2)
Activated
CYTOOPLATES?96RWY-S-A
20-010-00
Y
Small(700μm2)
Activated
CYTOOPLATES?96RWY-M-A
20-011-00
Y
Medium(1100μm2)
Activated
CYTOOPLATES?96RWY-L-A
20-012-00
Y
Large(1600μm2)
Activated
CYTOOPLATES?96RWSTARTER
20-900-00
Multiple
Multiple
Activated
CYTOOPLATES?96RWMOTILITYA
20-031-00
Line
Multiple
Activated
CYTOOchamber芯片槽
单孔芯片槽提供了大面积的成像区域，可承装10,000个细胞图形，这款产品尤其适合当您有超过4"个不同的几何图形打印在芯片上的时候，以及像是有丝分裂的细胞的特例测量。次产品更适用于高分辨率活细胞成像。
4孔芯片槽可提供每孔上千种图形在各情境下的平行免疫荧光实验，Arena芯片和Motility芯片的4象限设计使之适用于4孔芯片槽。
优势和主要特征:
货号
产品名称
图片
RD150
RoundDevice(150ummicrogroovebarrier)
Size:~21mmdiameter
Ourrounddeviceversionofthestandard150ummicrogroovebarrierhasanapproximatediameterof21mm,makingitcompatiblewithsome35mmglassbottomdishesthatmanyresearchersuseinlivecellimagingsystems.Glassbottomdishesusedshouldhaveaninnerapertureof21mmormoretoaccommodatethedevice.Atleast22mmisrecommendedinordertohaveroomtopositionthedevice.Largerglassbottomdishescanbeused,aswellasglasscoverslips,ifdesired.
RD450
RoundDevice(450ummicrogroovebarrier)
Size:~21mmdiameter
Ourrounddeviceversionofthestandard450ummicrogroovebarrierhasanapproximatediameterof21mm,makingitcompatiblewithsome35mmglassbottomdishesthatmanyresearchersuseinlivecellimagingsystems.Glassbottomdishesusedshouldhaveaninnerapertureof21mmormoretoaccommodatethedevice.Atleast22mmisrecommendedinordertohaveroomtopositionthedevice.Largerglassbottom"dishescanbeused,aswellasglasscoverslips,ifdesired.
RD900
RoundDevice(900ummicrogroovebarrier)
Size:~21mmdiameter
Ourrounddeviceversionofthestandard900ummicrogroovebarrierhasanapproximatediameterof21mm,makingitcompatiblewithsome35mmglassbottomdishesthatmanyresearchersuseinlivecellimagingsystems.Glassbottomdishesusedshouldhaveaninnerapertureof21mmor"moretoaccommodatethedevice.Atleast22mmisrecommendedinorderto"haveroomtopositionthedevice.Largerglassbottomdishescanbeused,as"wellasglasscoverslips,ifdesired.
SND150
StandardNeuronDevice(150ummicrogroovebarrier)
Size:~22mm–~23mm
The150umneurondevicewithitsshortermicrogroovebarrierissuitableforresearcherswhowouldliketoisolatebothaxonsanddendrites.Itisalsosuitableforcertain"neuronalculturesthatdonotgrowlongprocesses.
Researcherswhowishtostudytheearlyeventsofaxonalanddendriticgrowth"mayalsofindthe150umdeviceuseful.The150umdevice"alsooffersthebenefitsoffluidicisolationandcultureorganizationfortransportstudies.
SND450
StandardNeuronDevice(450ummicrogroovebarrier)
Size:~22mm–~23mm
"The450umneurondeviceisourmostpopulardevice.Formost"applicationsitissuitableforseparatingcellbodiesfromaxons.Aftertwoweeks,dendritesinE18ratcorticalneuronshavetypicallynotcrossedthemicrogroovebarrier.
"The450umneurondevicealsoprovidestheaddedfeaturesoffluidic"isolationandcultureorganizationfortransportstudies.
SND900
StandardNeuronDevice(900ummicrogroovebarrier)
Size:~22mm–~23mm
The900umneurondevice,withitswidemicrogroovebarrier,issuitableforresearcherswhowouldliketoperformlongtermexperimentswhileensuringaxonalisolation."Insomecultures,aftertwoweeksofgrowthdendritesmaycrossthe450umbarriertherebynecessitatingtheuseofthe900umdevice.Itisalsosuitable"forcertainneuronalcultureswhichgrowlongprocesses.Researcherswho"alsowishtomakesuretheyareisolating100%axonsonlyinthedistal"chambermayalsofindthe900umdeviceuseful.The900umdevicealsooffersthebenefitsoffluidicisolationandcultureorganizationfortransportstudies.
TCND500
TripleChamberNeuronDevice(500ummicrogroovebarrier)
Size:~38mm–~24mm
TheTCND500consistsof(2)500ummicrogroovebarrierswitha500umcentralchamber.Thisdevicemakesitpossibletoculturecortical"neuronsintheleftandrightchamberswhileculturinghippocampalneurons,orothercombinationsofcells,inthemiddle.Thetriplechamberisalsosuitablefortransportstudieswheretheresearcherwishestotreatthemiddleoftheaxongrowinginthemiddlechamberwhileobservingeffectsatthegrowthconeorinthesomalcompartment.Neuronscanalsobeloadedinthecentralchamberanddifferenttreatmentsappliedtotheleftandrightcompartments.
TCND1000
TripleChamberNeuronDevice(1000ummicrogroovebarrier)
Size:Size:~38mm–~24mm
TheTCND1000consistsof(2)500ummicrogroovebarrierswitha1000umcentralchamber.Thisdevicemakes"itpossibletoculturecorticalneuronsintheleftand"rightchamberswhileculturinghippocampalneurons,orothercombinationsofcells,inthemiddle.Thetriplechamberisalsosuitablefortransportstudieswheretheresearcherwishestotreatthemiddleoftheaxongrowinginthemiddlechamberwhileobservingeffectsatthegrowthconeorinthesomalcompartment.Neuronscanalsobeloadedinthecentralchamberanddifferenttreatments"appliedtotheleftandrightcompartments.
uLP
MicrofluidicLocalPerfusionDevicebarrier,(50ummicrogroove75micronperfusionchannel,500ummicrogroovebarrier)
Size:~40mm–~24mm
"OurMicrofluidicLocalPerfusionChamberwasfirstpublishedinNeuron."2010Apr15;66(1):57-68.Thisdevicehasa75micronperfusion"chamberwithasetofmicrogroovesontheleft50uminwidthandasetof"microgroovesontheright500uminwidth.Thisdevice,inconjuctionwitha"syringepumporothersuitableequipment,allowstheresearchertoperfuse"selectedtreatmentsandcompoundsacrossthetargetarea.Syringepumpor"othersuitableequipmentrequiredforproperuse.Formoreindepth"informationpleaseseeNeuron.2010Apr15;66(1):57-68.
