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RabbitMonoclonalRevmab/Anti-HumanIgG4FcRabbitMonoclonalAntibody,CloneRM217/31-1100-00/1Ea
RevMAbBiosciencesUSA,Inchasdevelopedarevolutionarymonoclonalantibodygenerationmethodwhichallowsdevelopmentofmonoclonalantibodieswithoutgenerationofhybridoma.Therecombinantantibodiesaredevelopedfromabroadrangeofantigen-...
ProductDetailsProductNameAnti-HumanIgG4FcRabbitMonoclonalAntibody[RM217]ProductDescriptionRabbitmonoclonaltoHumanIgG4FcCatalogNo.31-1100-00CloneNameRM217ProductDataSheetAnti-HumanIgG4FcRabbitMonoclonalAntibodyDataSheetRM217SpecificityRM217reactstotheFcregionofhumanIgG4.NocrossreactivitywithhumanIgG1,IgG2,IgG3,IgM,IgA,IgD,IgE,mouseIgG,ratIgG,orgoatIgG.ImmunogenPeptidecorrespondingtotheFcregionofHumanIgG4ApplicationFlowCytometry
ELISA
SpeciesReactivityHumanConjugateNoneIntendedUseResearchUseOnly.PropertiesFormLiquidStorageInstructionsStoreat-20.0°CStABIlityStablefor1Yearat-20.0°CfromdateofreceiptStorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazideVolume/ItemSize100ugConcentration1.0mg/mLPurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatantCloneTypeMonoclonalIsotypeRabbitIgGUsageELISA:50ng/well–200ng/well(forCapture);
0.05ug/mL–0.2ug/mL(forDetection).

Keywords.ProductImagesRM217

RM217specificallyreactstohumanIgG4.Nocross
reactivitywithhumanIgG1,IgG2,IgG3.
Sandwich

SandwichELISAusingRM217asthecapture
antibody,andBiotinylatedanti-humanlightchains
(κ+λ)antibodyRM129asthedetectionantibody,
followedbyanAPconjugatedstreptavidin.
Sandwich

SandwichELISAusingRM217asthecapture
antibody,andBiotinylatedanti-humanlightchains
(κ+λ)antibodyRM129asthedetectionantibody,
followedbyanAPconjugatedstreptavidin.
A

AtiterELISAusingRM217.Theplatewas
coatedwithdifferentamountsofhumanIgG4.
AserialdilutionofRM217wasusedasthe
primaryantibody.Analkalinephosphatase
conjugatedanti-rabbitIgGasthesecondary
antibody.

RevMAbBiosciencesUSA,Inchasdevelopedarevolutionarymonoclonalantibodygenerationmethodwhichallowsdevelopmentofmonoclonalantibodieswithoutgenerationofhybridoma.Therecombinantantibodiesaredevelopedfromabroadrangeofantigen-specificBcellsthatareinaccessibletotraditionalhybridomatechnology.Thisrevolutionarymethodhassuccessfullybeenappliedtodevelophighqualityrecombinantrabbitandgoatmonoclonalantibodies.


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