31105800S,RevMAb Biosciences,Rabbit Monoclonal,RevMAb Biosciences,+,兔单克隆,Array.Array.Array蚂蚁淘厂家直采.
Rabbit MonoclonalRevmab/Anti-Histone H3.3 Rabbit Monoclonal Antibody, Clone RM190/31-1058-00-S/25ug
RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-...
ProductDetailsProductNameAnti-HistoneH3.3RabbitMonoclonalAntibody[RM190]ProductDescriptionRabbitmonoclonaltoHistoneH3.3CatalogNo.31-1058-00CloneNameRM190ProductDataSheetAnti-HistoneH3.3RabbitMonoclonalAntibodyDataSheetRM190SpecificityRM190reactstoHistoneH3.3,independentofpost-translationalmodifications.NocrossreactivitywithHistoneH3.1orotherhistoneproteins.ImmunogenApeptidecorrespondingtohumanHistoneH3.3.ApplicationWesternBlot(WB)
ELISA
MultiplexSpeciesReactivityHuman
VertebratesConjugateNoneIntendedUseResearchUseOnly.PropertiesFormLiquidStorageInstructionsStoreat-20.0°CStABIlityStablefor1Yearat-20.0°CfromdateofreceiptStorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazideVolume/Size100ugConcentration1.0mg/mLPurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatantCloneTypeMonoclonalIsotypeRabbitIgGUsageWesternBlot:0.5ug/mL–2ug/mL;
ELISA:0.2ug/mL-1ug/mL;
Multiplex:0.05ug/mL–0.5ug/mL.
KeywordsReferencesforHistoneH3.3RabbitMonoclonalAntibody[RM190]HinchcliffeEHetal.Chromosomemissegregationduringanaphasetriggersp53cellcyclearrestthroughhistoneH3.3Ser31phosphorylation,NatCellBiol.,10.1038/ncb3348.(2016)IF;Human,Monkey.Readmore(PubMed:27136267)
ProductImages

RM190reactsspecificallytoHistoneH3.3.
No cross reactivity with Histone H3.1.

WesternBlotofHeLawholecelllysate,
recombinant Histone H3.3 and Histone H3.1
proteins, using RM190 at 1 ug/mL, showed
a band of histone H3.3 in HeLa cells and
recombinant H3.3 protein, and no cross
activity with Histone H3.1.
RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.