31105500,RevMAb Biosciences,Rabbit Monoclonal,RevMAb Biosciences,+,兔单克隆,Array.Array.Array蚂蚁淘厂家直采.

Rabbit MonoclonalRevmab/Anti-Acetyl-Histone H3 (Lys18) Rabbit Monoclonal Antibody, Clone RM166; H3K18ac, Histone H3 (K18 acetylation)/31-1055-
RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-...
ProductDetailsProductNameAnti-Acetyl-HistoneH3(Lys18)RabbitMonoclonalAntibody[RM166]ProductDescriptionRabbitmonoclonaltoAcetylatedHistoneH3Lysine18(K18ac),H3K18ac,HistoneH3(K18acetylation)CatalogNo.31-1055-00CloneNameRM166ProductDataSheetAnti-Acetyl-HistoneH3Lys18RabbitMonoclonalAntibodyDataSheetRM166SpecificityRM166reactstoHistoneH3acetylatedatLysine18(K18ac).NocrossreactivitywithotheracetylatedLysinesinhistoneH3.ImmunogenAnacetyl-peptidecorrespondingtoAcetyl-HistoneH3(Lys18).ApplicationWesternBlot(WB)
ChromatinIP(ChIP)
Immunocytochemistry(ICC)
Immunohistochemistry(IHC)
ELISA
Multiplex
SpeciesReactivityHuman
VertebratesConjugateNoneIntendedUseResearchUseOnly.PropertiesFormLiquidStorageInstructionsStoreat-20.0°CStABIlityStablefor1Yearat-20.0°CfromdateofreceiptStorageBuffer50%Glycerol/PBSwith1%BSAand0.09%sodiumazideVolume/Size100ugConcentration1.0mg/mLPurityProteinAaffinitypurifiedfromananimalorigin–freeculturesupernatantCloneTypeMonoclonalIsotypeRabbitIgGUsageWesternBlot:0.5ug/mL-2ug/mL;
ICC:0.5ug/mL-2ug/mL;
ChIP:2ug/mL-10ug/mL;
IHC:1ug/mL-10ug/mL;
ELISA:0.2ug/mL-1ug/mL;
Multiplex:0.1ug/mL–0.5ug/mL.

KeywordsReferencesforAcetyl-HistoneH3(Lys18)antibody[RM166]LiY.etal.ActivationofmutantTERTpromoterbyRAS-ERKsignalingisakeystepinmalignantprogressionofBRAF-mutanthumanmelanomas.ProcNatlAcadSciUSA.113(50):14402-14407.ChIP;Human.Readmore(PubMed:27911794)ProductImages

RM166specificallyreactstoHistoneH3
acetylated at Lysine 18 (K18ac). No cross reactivity
with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac),
Lysine 14 (K14ac), Lysine 23 (K23ac), Lysine 27
(K27ac), Lysine 36 (K36ac), Lysine 56 (K56ac),
Lysine 79 (K79ac), or Lysine 122 (K122) in Histone H3.

WesternBlotofacidextractsfromHeLacells
untreated (-) or treated with sodium butyrate (+),
using RM166 at 0.5 ug/mL, showed a band of
histone H3 acetylated at Lysine 18 in treated HeLa.

SandwichELISAagainstacetylatedhistoneH3
at Lys 18 using HeLa whole cell lysate, treated or
untreated with Sodium Butyrate. Using anti-H3CT
(RM188, 1 ug/mL) as the capture antibody and
biotinylated anti-H3K18C (RM166, 2 ug/mL) as the
detection antibody.

ChIPperformedonHeLacellswithorwithout
Sodium Butyrate treatment, using H3K18Ac
antibody (RM166, 5ug). Real-time PCR was
performed using primers specific to the gene
indicated.

ImmunocytochemistryofHeLacellstreatedwith
sodium butyrate, using Acetyl-Histone H3 (Lys18)
Rabbit mAb RM166(red). Actin filaments have
been labeled with fluorescein phalloidin (green).

ImmunohistochemistrystainingofHepG2cellsusing
Anti-Acetyl-Histone H3 (Lys18) antibody, RM166.

RevMAb Biosciences USA, Inc has developed a revolutionary monoclonal antibody generation method which allows development of monoclonal antibodies without generation of hybridoma. The recombinant antibodies are developed from a broad range of antigen-specific B cells that are inaccessible to traditional hybridoma technology. This revolutionary method has successfully been applied to develop high quality recombinant rabbit and goat monoclonal antibodies.