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MCLAB/Exonuclease I, E. coli/NI-OEM/Any Size

ExonucleaseIcleavessingle-strandedDNAinthe3′→5′direction,releasing5′-mono/di-nucleotidesandleavingdouble-strandedDNAmoleculesandthe5'-terminusintact.

Description:
ExonucleaseIcleavessingle-strandedDNAinthe3'->5'direction,releasing5'-mono/di-nucleotidesandleavingdouble-strandedDNAmoleculesandthe5'-terminusintact.Theenzymeisprocessedthroughdigestionandisinhibitedbythepresenceofa3'-terminalphosphate.ExonucleaseIistolerantofawide-rangeofbufferconditionsandcantypicallybeaddedtoreactionscontainingmagnesium(1-3).

SpecificActivity:185,000U/mg

Application:
-RemovalofresidualssDNA,includingoligos,fromreactionmixes

Source:
PurifiedfromastrainofE.colithatexpressestherecombinantExonucleaseIgene.

Suppliedin:
10mMTris-HCl
100mMNaCl
1mMDTT
0.5mMEDTA
50%Glycerol
pH7.5@25°C
 

10xExonucleaseIBuffer
670mMGlycine-KOH
67mMMgCl2
100mM2-mercaptoethanol
(pH9.5@25°C)

UnitDefinition:
Oneunitisdefinedastheamountofenzymerequiredtoproduce10nmolofacid-solubletotalnucleotidein30minutesat37°C.

RecommendedStorageCondition:-20°C

Reference:
1.Lehman,I.R.andNussbaum,A.L.(1964)J.Biol.Chem.239,2628.
2.Kushner,S.R.etal.(1971)Proc.Natl.Acad.Sci.USA68,824.
3.Kushner,S.R.etal.(1972)Proc.Natl.Acad.Sci.USA69,1366.

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