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MCLAB/BrightDye® Terminator Cycle Sequencing Kit/BDT3-24/1 Ea

TheBrightDye®TerminatorCycleSequencingKitisdesignedfordenovosequencing,resequencing, PCRproduct,plasmid,fosmid,andBACtemplatesbyutilizinghighlyflexIBLechemistry.

Description:
TheBrightDye®TerminatorCycleSequencingKitisdesignedfordenovosequencing,resequencing, PCRproduct,plasmid,fosmid,andBACtemplatesbyutilizinghighlyflexiblechemistry.ThiskitusesfourdifferentfluorescentdyestolabelddNTPs,whichareaddedsequentiallytotheprimerthroughacyclesequencingreaction.Allrequiredreagentsforthesequencingprocedureareinareaction-ready,pre-mixedformat.BrightDyeterminatorkitiscompatiblewithcommerciallyavailableBigDyeterminatorV3.1kit,withoutanychangesintheworkflow.
Withenhancedrobustnessandbetterdyemobilitycharacteristics,theBrightDye®TerminatorCycleSequencingKitcanbeusedinawiderangeofapplicationswiththefollowingfeatures:

  • Longreadlengths.
  • BetterperformancereADIngthroughGCrichregions.
  • Longerreadswithmoreuniformpeakheights.
  • Improvedproductivityforlesscost.
Applications:
Denovosequencing
Resequencing
Sequencingdifficulttemplates
Long-readsequencing
Sequencingacrossalltemplatetypes(plasmids,PCRproducts,BACs,andfosmids)
Mixed-basedetection
SequencingshortPCRproductsusingrapidelectrophoresisrunmodules

Platforms:
GeneAmp®9700,Veriti®ThermalCycler,3730DNAAnalyzer,3500xLGeneticAnalyzer,3500GeneticAnalyzer,310GeneticAnalyzer,3130GeneticAnalyzer,3130xlGeneticAnalyzer,3730xlDNAAnalyzer

TemplateCompatibility:
PlasmidDNA(≤15Kb)
Fosmids
SingleStrandedDNA
LamBDaDNA
BACDNA
GenomicDNA(Bacterial)
PCRAmplicons

Storage:
Storekitat-20°C.Avoidrepeatedfreeze-thawcycles.

RegulatoryStatement:
ForResearchUseOnly.Notforuseindiagnosticprocedures.

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