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lumiprobe/FAM amine, 5-isomer/241C0/5 mg

Fluorescein(FAM)amine,isomericallypuredye(5-isomer).Unlikefluoresceinamine,thisreagentcontainsalinkerarmwithanaliphaticaminegroupthatismorereactive.Itcanreactwithelectrophilicreagentslikeactivatedestersorepoxides,andbeinvolvedinenzymatictransamination.

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Generalproperties

Appearance:yellowsolid
Molecularweight:510.97
Molecularformula:C27H27N2ClO6
IUPACname:6-aminohexylaminocarbonylfluoresceinhydrochloride
Solubility:goodinmethanol,DMSO,DMF
Qualitycontrol:NMR1H,HPLC-MS(95%)
Storageconditions:Storage:24monthsafterreceivalat-20°Cinthedark.Transportation:atroomtemperatureforupto3weeks.Avoidprolongedexposuretolight.Desiccate.

Spectralproperties

Excitationmaximum,nm:490
ε,L⋅mol−1⋅cm−1:80000
Emissionmaximum,nm:513
Fluorescencequantumyield:0.93
CF260:0.20
CF280:0.17
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