Blocking step BSA as blocking agent for 18 hour(s) and 0 minute(s) Concentration: 5% Temperature: 4 C Incubation time 1 hour(s) and 30 minute(s) Temperature: 21 C Diluent: PBS + 0.1% Tween20 Name Non-Abcam antibody was used: Anti Rabbit IgG Host species: Goat Clonality: Polyclonal Conjugation: Cy5 Negative control There was no negative control, as all of the samples should have been positive Additional Notes We were having trouble finding a WB housekeeping gene for our cell line EfK3B (Big Brown Bat cells), which we immortalized in our laboratory recently, so very little is known about the characteristics of them. We were very thrilled to discover that B-actin (ab8227) cross-reacted with them, so we will use this antibody for our future work. This cell line is available commercially through KeraFastMolecular Weight Marker used is Bio-Rad Precision Plus Protein WesternC #1610376All lanes contain the same amount of whole cell lysate and no non-specific bands were seen.Since this was a dual stain experiment, with treated and untreated cells, I also have images with both colors available, but I only included the B-actin image.
