PAI ACTIVITY ASSAY KIT

PAIActivityAssay
Description:
PAIActivityAssay
TradeName:
Chemicon(Millipore)
Qty/Pk:
1plate
ProductOverview:
TheCHEMICONPAIActivityAssayKitprovidesaquick,efficientandsensitivesystemforevaluationofinhibitionofuPAactivitybyPAIandforscreeningofuPAinhibitors.TheassayiscolorimetricanddoesnotrequirerADIoactivityorfluorescenceequipment.Theassayissensitiveoveraninhibitionrangeof0.05-50unitsofuPAactivity. 
TheCHEMICONPAIActivityAssayKitutilizesachromogenicsubstrate,whichiscleavedbyactiveuPA,detectablebyitsOpticalDensityat405nm(OD405).AdditionofPAIsampleblocksthecleavageofsubstratebyuPA. 
TheCHEMICONPAIAssayKitisidealformeasurementofPAIinhibitoryactivitytowarduPAinpurifiedpreparationsandcellculture,aswellasinserumwherepathologicalconditionssuchassepsisexist.TheassayisalsousefulforscreeninginhibitorsofuPA. 
EachCHEMICONPAIActivityAssayKitcontainssufficientreagentsfortheevaluationof96samples,includinguPAstandardfromhumanurineandE.Coli-expressedrecombinantproteincorrespondingtoaminoacids24to427ofhumanPAI-1(matureform)aspositivecontrol.PAI-1positivecontrolcontainsamixtureoftheactiveandlatentforms,with>45%ofactiveform.Duplicateortriplicatesamplesaresuggested. 
TheCHEMICONPAIActivityAssayKitisintendedforresearchuseonly;notfordiagnosticortherapeuticapplications. 
TestPrinciple: 
TheCHEMICONPAIActivityAssayKitutilizesachromogenicsubstrate,whichiscleavedbyactiveuPA,detectablebyitsOpticalDensityat405nm(OD405).AdditionofPAIsampleblocksthecleavageofsubstratebyuPA. 
Application: 
TheCHEMICONPAIAssayKitisidealformeasurementofPAIinhibitoryactivitytowarduPAinpurifiedpreparationsandcellculture,aswellasinserumwherepathologicalconditionssuchassepsisexist.TheassayisalsousefulforscreeninginhibitorsofuPA. 
EachCHEMICONPAIActivityAssayKitcontainssufficientreagentsfortheevaluationof96samples,includinguPAstandardfromhumanurineandE.coliexpressedrecombinantproteincorrespondingtoaminoacids24to427ofhumanPAI-1(matureform)aspositivecontrol.PAI-1positivecontrolcontainsamixtureoftheactiveandlatentforms,with>45%ofactiveform.Duplicateortriplicatesamplesaresuggested. 
TheCHEMICONPAIActivityAssayKitisintendedforresearchuseonly;notfordiagnosticortherapeuticapplications.
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Applications:
ThePAIActivityAssayKitprovidesaquick,efficient&sensitivesystemforevaluationofinhibitionofuPAactivitybyPAI&forscreeningofuPAinhibitors.
KeyApplications:
ActivityAssay
SpeciesReactivity:
Human
UsageStatement:
Unlessotherwisestatedinourcatalogorothercompanydocumentationaccompanyingtheproduct(s),ourproductsareintendedforresearchuseonlyandarenottobeusedforanyotherpurpose,whichincludesbutisnotlimitedto,unauthorizedcommercialuses,invitrodiagnosticuses,exvivoorinvivotherapeuticusesoranytypeofconsumptionorapplicationtohumansoranimals.
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EntrezGeneSummary:
ThisgeneencodesaserineproteaseinvolvedindegradationoftheextracellularmatrixandpossIBLytumorcellmigrationandproliferation.Aspecificpolymorphisminthisgenemaybeassociatedwithlate-onsetAlzheimerdiseaseandalsowithdecreasedaffinityforfibrin-binding.TheproteinencodedbythisgeneconvertsplasminogentoplasminbyspecificcleavageofanArg-Valbondinplasminogen.Thisgene"sproproteiniscleavedataLys-Ilebondbyplasmintoformatwo-chainderivativeinwhichasingledisulfidebondconnectsth