Kapa biosystems/KAPA Stranded mRNASeq Kits/KK8721/25 mL

Description:

KAPAAdapterDilutionBuffer(25mL)

KAPAStrandedmRNA-SeqKits

Evendifficultmessagesshouldbeunderstood.

KAPAStrandedmRNA-SeqKitsincludealltheenzymesandbuffersrequiredforcDNAlibrarypreparationforIlluminaNext-GenerationSequencing,utilizing100ng–4µgoftotalRNA.KAPAmRNACaptureBeadsareincludedforisolationofpoly(A)-tailedRNA.Kitsprovideprecisemeasurementofstrandorientation(>99%),uniformcoverage,andhigh-confidencemappingofalternatetranscripts,andareoptimizedfortheimprovedcoverageofGC-richandlow-abundancetranscripts.*KitscontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,aswellasKAPAmRNACaptureBeadsandastreamlined,“with-bead”protocol.

KAPAStrandedRNA-SeqKitsincludealltheenzymesandbuffersrequiredforcDNAlibrarypreparationforIlluminaNext-GenerationSequencing,butdonotcontaintheKAPAmRNACaptureBeads.Kitscanbeusedtopreparelibrariesfrom10–400ngofeitherpoly(A)-selected,ribosomallydepleted,ortotalRNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

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_*Dataonfile.ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

Improved coverage of GC-rich transcripts. The 5 and 3 exons (outlined in red) of the DVL3 transcript contain regions of very high GC content. These regions are covered to a significantly greater depth by the KAPA Stranded mRNA-Seq Kit (green) in comparison to the Illumina® TruSeq™ Stranded mRNA Sample Prep Kit (orange). Data on file.

Uncoverchallengingtranscripts

ImprovedcoverageofGC-richtranscripts
Enhancedidentificationofexonicregions

Improved coverage of low-abundance transcripts. GLTPD1, a lesser-expressed transcript, is covered more comprehensively with the KAPA Stranded mRNA-Seq Kit (green) at 500 ng input total RNA (outlined in red). In comparison, Illumina TruSeq Stranded mRNA Sample Prep Kit (orange) shows coverage gaps, even with higher inputs (4 μg). Data on file.

Detectlow-abundancetranscripts

Enablesidentificationoftranscriptsmissedbycompetitorkits,evenwithhighinput*
Highuniformityacrossvaryingamountsofsampleinput*

30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile."> Highly mapped reads and strand specificity enable sensitive detection of expressed genes. With similar numbers of filter-passed reads (>30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile.

Highly mapped reads and strand specificity enable sensitive detection of expressed genes. With similar numbers of filter-passed reads (>30M),KAPAStrandedmRNA-SeqlibrariesgenerateagreaterpercentageofmappedreadsandlowerduplicationratesthananalogouslibrariespreparedwiththeIlluminaStrandedmRNASamplePrepKitwhilemaintaining99%strandspecificity.Dataonfile.

Identifymoregenes

HigherpercentageofuniquelymappedreadscomparedtoIllumina TruSeq™StrandedmRNASamplePrepKits*
Lowerduplicationratesyieldbettercoverage

Minimal positional bias. With intact, high-quality RNA input, 3 positional coverage bias was minimized (average across top 1000 transcripts shown). Compared to the Illumina TruSeq Stranded mRNA Sample Prep Kit (orange), KAPA Stranded mRNA-Seq Kit (green) produces equivalent 5’ – 3’ coverage across transcript. Data on file.

Maintainhighcoverageuniformity

Minimal5’–3′biasacrosstranscripts
Moreuniformdistributionofreadsovereachtranscript

_*Dataonfile.

RelatedProducts

SequencingchallengingRNAsamples?HowaboutDNA?CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPAhgDNAQuantificationandQCKit

KAPALibraryAmplificationKits

KAPAHyperPrepKits

KAPALibraryQuantificationKits

Applications:

Applications

Geneexpression
Singlenucleotidevariation(SNV)discovery
Post-transcriptionalSNVs
Fusiongeneidentification
Targetedtranscriptome
Wholetranscriptome

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto8 months at-20˚C. KAPAmRNACaptureBeadscanbestoredforupto8months at-4˚C.

Components

mRNA_Component Chart

Specifications

CompatiblePlatform

IlluminaHiSeq,NextSeq,MiSeqandGAIIx

StartingMaterial

TotalRNA,mRNA,orribosomaldepletedRNA

InputAmount

StrandedmRNA-SeqKits:100ng–4µgStrandedRNA-SeqKits:10ng–400ng

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