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Kapa biosystems/KAPA Hyper Prep Kits/KK8500/8 reactions

Description:

WithKAPALibraryAmplificationPrimerMix(10X)

KAPAHyperPrepKits

Shiftyourworkflowintohyperdrive.

TheKAPAHyperPrepKitisaversatile,streamlinedsolutionforDNAlibrarypreparationforIllumina®sequencing.

Thenovelone-tubechemistry,containoptimallyformulatedandevolvedenzymesthatenablehigheryieldsofadapter-ligatedlibraryandloweramplificationbias.Thistranslatestohigherlibrarydiversity,lowerduplicationratesandmoreuniformcoverage,particularlyforFFPEandlow-inputsamples.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

 

ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

KAPA Hyper Prep Kits convert more input DNA to adapter-ligated library. Libraries were prepared from Covaris-sheared DNA and quantified prior to library amplification with the KAPA Library Quantification Kit. The KAPA Hyper Prep Kits achieved the highest yield of adapter-ligated library across the range of inputs (1 μg, 100 ng or 10 ng), and required fewer cycles of amplification to generate 1 μg of amplified library required for target capture. Data on file.
Number of amplification cycles needed to generate ≥1 µg amplified library for target capture with the SeqCap EZ Comprehensive Cancer Design (578 genes, 4 MB; Roche Nimblegen), for libraries prepared with each kit. The higher conversion rates achieved with the KAPA Hyper Prep Kit, combined with high-efficiency, low-bias amplification with KAPA HiFi DNA Polymerase, translates to significantly less cycles of pre-capture amplification and subsequent improvements in sequence data quality. Data on file.
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Improvelibraryyieldsandsequencequality

  • Higherlibraryyieldstranslatetogreatermolecularcomplexity
  • FewercyclesofamplificationwithKAPAHiFiDNAPolymeraseresultsinlowerduplicationratesandimprovedcoverage
Sequencing metrics FFPE libraries prepared for whole exome sequencing. Libraries were prepared from 100 ng of FFPE DNA, and exome capture was performed with the SeqCap EZ HGSC VCRome Design, Roche NimbleGen. Higher library yields enabled fewer cycles of amplification, leading to significantly lower duplication rates and higher target coverage. Data on file.
Conversion rates for FFPE DNA has been identified as a primary bottleneck in NGS library construction. With the KAPA Hyper Prep Kit, conversion rates for FFPE samples are similar to those for high-quality DNA. This leads to significantly lower duplication rates and higher overall target coverage in exome and other target capture workflows. Conversion data are for libraries prepared from 80 – 100 ng. Covaris-sheared FFPE DNA of variable quality, or 100 ng control DNA. Data on file.
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Createhigh-qualitylibrariesfromFFPEsamples

  • Generatehigh-qualitylibrariesfrom250ngFFPEDNAorless*
  • Significantlylowerduplicationratesandhighercoverage*
The streamlined KAPA Hyper Prep workflow. End repair, A-tailing and ligation is performed in a single tube, without intervening cleanup steps. The workflow is ideally suited for robust, high-throughput sample preparation for Illumina sequencing. Data on file.

Completelibraryconstructioninlessthan3hours

  • One-tubeworkflowwithminimalcleanupstepsreducesoveralltime,andminimizeshands-ontime
  • Sample-to-libraryin<2hoursforPCR-freeworkflows,or<3hourswithamplification*
  • Fewerhandlingstepsleadtoimprovedconsistencyandreproducibility
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries were amplified with the Real-Time library Amplification Kit, prior to sequencing on an Illumina MiSeq. With the novel KAPA Hyper Prep chemistry, higher adapter concentrations can be used to improve library diversity. Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries were amplified with the Real-Time library Amplification Kit, prior to sequencing on an Illumina MiSeq. With the novel KAPA Hyper Prep chemistry, higher adapter concentrations can be used to reduce duplication rates. Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries prepared with the two kits had similar insert sizes (D). Data on file.
Key sequencing metrics for libraries prepared from 2 or 10 ng free-circulating DNA from plasma, using the KAPA Hyper Prep Kit or KAPA LTP Library Preparation Kit, with different adapter:insert molar ratios. Libraries prepared with the two kits had similar insert sizes (D). Data on file.
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Improveperformancewithlow-inputsamples

  • Generatemoresequence-qualitylibrarymoleculeswithoutincreasingadapter-dimers
  • Increaseyieldswithoutinducingsizebias

 

*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

Sample QC

KAPAhgDNAQuantificationandQCKit

Library Amplification

KAPALibraryAmplificationKits

Library Quantification

KAPALibraryQuantificationKits

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

Applications:

Applications
  • WholeGenomeSequencing
  • WholeExomeSequencing
  • TargetedSequencing(custompanels)
  • ChIP-seq
  • AmpliconSequencing
  • Methyl-seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto12months at-20˚C.

Kitscontain KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix

Components

Hyper_Component Chart

Specifications

Spec
Description
CompatiblePlatform
lluminaHiSeq,MiSeq,NextSeqandGAIIx
LibraryType
DNA
StartingMaterial
FragmentedDNA,cDNA,orPCRamplicons
InputAmount
1ng–1µg

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