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Kapa biosystems/Library Preparation for Illumina/KK8230/8 reactions

Description:

Lowthroughput,"withbead"

LibraryPreparationforIllumina

It’scomplex,butwehaveitcovered.

KAPADNALibraryPreparationKitsforIllumina®sequencingplatformscontainevolvedandoptimallyformulatedenzymesthatenablethehighestoverallcoveragefromtheleastamountoftotalsequencing.

KitsareoptimizedtoachievesignificantlyhigherlibraryyieldsandcontainKAPAHiFiforhigh-fidelity,high-efficiencyandlow-biaslibraryamplification.Thisensuresthehighestlibraryandsequencedataquality,particularlyfromlow-inputanddifficultsamplessuchasFFPEandChIPDNA.

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourKAPA AdapterandBeadCalculator

 

ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

Key sequencing metrics (library diversity, left and duplication rates, right) for libraries prepared for exome capture (SureSelect All Human Exome V5 + UTR, Agilent Technologies) from different amounts of Covaris-sheared DNA using the KAPA Library Preparation Kit (green) or a competitor kit (blue). Optimally formulated and evolved enzymes, combined with a highly optimized “with-bead” protocol result in higher library yields with the KAPA Library Preparation Kit. This translates to higher library diversity and lower duplication rates, even with significantly less input DNA. Paired-end sequencing (2 x 100 bp) was performed on an Illumina HiSeq 2000. Data courtesy of the Translational Genomics Research Institute. Data on file.
Key sequencing metrics (library diversity, left and duplication rates, right) for libraries prepared for exome capture (SureSelect All Human Exome V5 + UTR, Agilent Technologies) from different amounts of Covaris-sheared DNA using the KAPA Library Preparation Kit (green) or a competitor kit (blue). Optimally formulated and evolved enzymes, combined with a highly optimized “with-bead” protocol result in higher library yields with the KAPA Library Preparation Kit. This translates to higher library diversity and lower duplication rates, even with significantly less input DNA. Paired-end sequencing (2 x 100 bp) was performed on an Illumina HiSeq 2000. Data courtesy of the Translational Genomics Research Institute. Data on file.
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Achievegreatermolecularcomplexity

  • Higheryieldsofadapter-ligatedlibrarytranslatestohighermolecularcomplexity
  • Fewercyclesofamplificationareneeded,whichresultsinlowerPCRduplicationrates
  • PCR-freeworkflowspossiblefrominputs≥100ng*
Indexed libraries were constructed and amplified from 100 ng human genomic DNA using either the KAPA Library Preparation Kit (right) or the standard library preparation reagents and protocol in use at The Broad Institute (left). Individual libraries were quantified using qPCR, pooled prior to denaturation, and sequenced (pair-ended, 2 x 25 bp) on an Illumina HiSeq 2000. Libraries prepared using the KAPA Library Preparation Kit resulted in more uniform coverage distribution across the range of GC-content. Data courtesy of The Broad Institute. Data on file.
Indexed libraries were constructed and amplified from 100 ng human genomic DNA using either the KAPA Library Preparation Kit (right) or the standard library preparation reagents and protocol in use at The Broad Institute (left). Individual libraries were quantified using qPCR, pooled prior to denaturation, and sequenced (pair-ended, 2 x 25 bp) on an Illumina HiSeq 2000. Libraries prepared using the KAPA Library Preparation Kit resulted in more uniform coverage distribution across the range of GC-content. Data courtesy of The Broad Institute. Data on file.
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Reduceamplificationbiasandimprovecoverage

  • KAPAHiFireducesPCRbias*
  • ImprovescoverageuniformityofGC-andAT-richregions,promoters,lowcomplexityandotherchallengingregions*
Key sequencing metrics (fraction of duplicates, left and post-capture library complexity, right) for FFPE libraries prepared with the KAPA Library Preparation Kit for target capture (SeqCap EZ custom panel, 279 genes, 1 MB; Roche Nimblegen). Of each of the eight colon/gastric cancer FFPE samples, 250 ng FFPE DNA was Covaris-sheared for automated library construction, using a Biomek FXp instrument (Beckman Coulter). KAPA HiFi HotStart ReadyMix was used for both pre- and post-capture amplification (8 and 12 cycles, respectively). Paired-end sequencing (2 x 75 bp) was performed on an Illumina MiSeq. Data courtesy of Memorial Sloane-Kettering Cancer Center. Data on file.
Key sequencing metrics (fraction of duplicates, left and post-capture library complexity, right) for FFPE libraries prepared with the KAPA Library Preparation Kit for target capture (SeqCap EZ custom panel, 279 genes, 1 MB; Roche Nimblegen). Of each of the eight colon/gastric cancer FFPE samples, 250 ng FFPE DNA was Covaris-sheared for automated library construction, using a Biomek FXp instrument (Beckman Coulter). KAPA HiFi HotStart ReadyMix was used for both pre- and post-capture amplification (8 and 12 cycles, respectively). Paired-end sequencing (2 x 75 bp) was performed on an Illumina MiSeq. Data courtesy of Memorial Sloane-Kettering Cancer Center. Data on file.
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Createhigh-qualitylibrariesfromchallengingsamples

  • High-qualitywholeexomesequencingfromlibrariespreparedusingaslittleas10nghumangenomicDNA*
  • Highsuccessrateswith250ngFFPEorless*
  • Routinelibraryconstructionfrom≥100pgChIPDNA*
KAPA Library Quantification methods (96- and 384-well) format are available for the Sciclone NGS and NGSx workstation (PerkinElmer) and other instruments used in high-throughput NGS sample preparation pipelines.

Automatewithpre-validatedscripts

  • PerkinElmerSciclone®NGSandNGSx
  • BeckmanCoulter®Biomek™FXpandFXdual-pod
  • Agilent®TechnologiesNGSWorkstationOptionB
  • Eppendorf®epMotion™ 5075TMX

 

*Dataonfile.

Applications:

Applications
  • WholeGenomeSequencing
  • ChIPSequencing
  • WholeExomeSequencing
  • AmpliconSequencing
  • TargetedSequencing(Capture)
  • MethylSequencing

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto18months at-20˚C.

Kitsincludereagentsforendrepair,A-tailing,ligationandlibraryamplification.PCR-freeversions(withoutlibraryamplificationreagent)arealsoavailable.“With-bead”kitsincludePEG/NaClsolutionforSPRIbeadcleanups.
All96-librarykitsareautomationfriendly.

Components

Specifications

Spec
Description
CompatiblePlatform
IlluminaHiSeq,MiSeq,NextSeqandGAIIx
LibraryType
DNA
StartingMaterial
FragmentedDNAorPCRamplicons
InputAmount
100pg–5µg
SequencingApplications
WholeGenomeSequencingWholeExomeSequencingTargetedSequencing(custompanels)ChIP-SeqAmpliconSequencingMethyl-Seq

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