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Kapa biosystems/KAPA Stranded RNASeq with RiboErase/KK8712/12 adapters x 40 µl each

Description:

KAPASingle-IndexedAdapterKit,SetB(1.5µM)

KAPAStrandedRNA-SeqwithRiboErase

Evolvedtofocus.

TheKAPAStrandedRNA-SeqKitwithRiboEraseoffersahigh-quality,comprehensivesolutionfortranscriptomesequencing.Byutilizingatargetedenzymaticmethodfordepletion,ourworkflowenablessuperiorreductionofribosomalRNA(rRNA)andamorecompleterepresentationofthetranscriptome,includingprecursormRNAsandnon-codingRNA(ncRNA).KitsalsocontainKAPAHiFiforhigh-efficiencyandlow-biaslibraryamplification,andincludeastreamlined,“with-bead”protocol.

  • Upto99.98%rRNAdepletion*
  • Flexibleinputof100ng–1μgtotalRNAfromhuman,mouse,orratspecies*
  • Robustandreproducibleacrossvariousinputamounts
  • Automation-friendlyworkflow

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

DownloadourAdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

ProductHighlights

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IndustryleadingrRNAdepletion

  • SuperiorrRNAdepletionfromhigh-qualityandFFPEsamples*
  • MoreeconomicalNGSsequencingduetodecreasedrRNAreads,providingdeepersequencingoftranscriptsofinterest
High-quality sequencing data obtained from KAPA Stranded RNA-Seq Kit with RiboErase when compared to the equivalent Illumina or NEB kits. With equivalent mapping rates, KAPA Stranded RNA-Seq Kit with RiboErase provides lower duplication rates, better coverage uniformity and consistently detects more unique transcripts and genes. This enables a more sensitive detection of the expressed genes within the sample. Data on file.
Improved coverage of GC-rich transcripts. IGV image showing coverage and splice junction tracks of the haemoglobin alpha transcript (HBA1) which has a GC content of 62.8%. The GC content track shows GC-rich regions in red, and AT-rich regions in blue. More even coverage, improved splice-site recognition, and more representative plot compared to the undepleted sample (red) is seen when libraries are prepared with the KAPA Stranded RNA-Seq Kit with RiboErase (green) compared to the equivalent Illumina (orange) or NEB (blue) kits. Data on file.
Better detection of low-abundance transcripts. IGV image showing coverage and splice junction tracks of MALAT1, a low abundance long non-coding RNA transcript, which is more comprehensively covered with use of KAPA Stranded RNA-Seq Kit with RiboErase (green) for library preparation. In comparison, Illumina Ribo-Zero Gold (orange) and NEBNext (blue) show less even coverage of this transcript. Data on file.
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Unsurpassedsequencingdataquality

  • Detectionofmoregenesanduniquetranscripts
  • Accurateandclearidentificationofsplicesitesandalternativegenesplicing
  • ImprovedcoverageenablingbetterdetectionofdifficultandGC-richtranscripts
Hierarchically clustered heatmap showing the correlations of gene expression levels between different laboratories and RNA input amounts. All correlations were greater than 0.93, with technical replicates produced in the same lab exceeding 0.98. Generally, all libraries from a single lab, including those produced from different input amounts, clustered together. The data indicates that the KAPA Stranded RNA-Seq Kit with RiboErase delivers robust, reproducible, and unbiased gene expression data from a wide range of input amounts. Data on file.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.">Correlation of gene expression levels between replicate samples. Libraries prepared using 100 ng of UHR Total RNA with the KAPA Stranded RNA-Seq Kit with RiboErase show a very high correlation (R2>0.973)whicharebetterthanlibrariespreparedfromtheequivalentkitsfromIlluminaorNEB.Dataonfile.
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Highlyreproduciblesequencingresults

  • Highcorrelationevenbetweendifferenttestingconditions
  • Lowsample-to-samplevariationformorereliableresults
Improved coverage of the whole transcript. The housekeeping gene Beta-Actin has improved coverage at the 3’ and 5’ regions using the KAPA Stranded RNA-Seq Kit with RiboErase (green) when compared to the equivalent Illumina Ribo-Zero Gold (orange) and NEBNext (blue) kits. Data on file.
Minimal positional bias. With 100 ng of high-quality RNA input, 3 positional coverage bias is minimized. Compared to Illumina Ribo-Zero Gold (orange) and the undepleted sample (magenta), KAPA Stranded RNA-Seq Kit with RiboErase (green) produces equivalent 5’ – 3’ coverage across transcript. When compared to NEBNext (blue), the KAPA Stranded RNA-Seq Kit with RiboErase provides more uniform distribution of reads per transcript. Data on file.
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Improvedcoverageuniformity

  • Uniformdistributionofreadsovereachtranscript
  • Minimal5’–3’biasacrosstranscripts


*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

KAPA RNA HyperPrep Kits with RiboErase

KAPARNAHyperPrepKitswithRiboErase

KAPA mRNA HyperPrep Kits

KAPAmRNAHyperPrepKits

Library Amplification

KAPALibraryAmplificationKits

Library Preparation

KAPAHyperPrepKits

Library Quantification

KAPALibraryQuantificationKits

Applications:

Applications
  • WholeTranscriptome
  • GeneExpression
  • TranscriptDiscoveryandAnnotation
  • TotalRNA-Seq

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto10monthsat-20ºC.

KitscontainKAPAHybridizationBuffer,KAPAHybridizationOligos(Human/Mouse/Rat),KAPADepletionBuffer,KAPARNaseH,KAPADNaseBuffer,KAPADnase,KAPAFragment,PrimeandEluteBuffer(2X),KAPA1stStrandSynthesisBuffer,KAPAScript,KAPA2ndStrandMarkingBuffer,KAPA2ndStrandSynthesisEnzymeMix,KAPAA-TailingBuffer(10X),KAPAA-TailingEnzyme,KAPALigationBuffer(5X),KAPADNALigase,KAPAPEG/NaClSPRISolution,KAPALibraryAmplificationPrimerMix(10X)andKAPAHiFiHotStartReadyMix(2X).

Components

RIBO-Component Chart

Specifications

Spec
Description
CompatiblePlatform
IlluminaHiSeq®,NextSeq®andMiSeq®
LibraryType
RNA
StartingMaterial
High-qualityTotalRNA
InputAmount
100ng-1µg

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