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Kapa biosystems/KAPA HyperPlus Kits/KK8514/96 reactions

Description:

WithLibraryAmplification

KAPAHyperPlusKits

NGSlibrarypreparation. Evolved.

TheKAPAHyperPluskitprovidesastreamlinedworkflowthatincludesfragmentationandlibrarypreparationinasingletube.Buildingonindustry-leadinglibraryconstructionefficiencies,thisintegratedsolutioncombinesenzymaticfragmentation,similarinqualitytomechanicalshearing,withthespeedandconvenienceoftagmentation-basedworkflows.Benefitsinclude:

  • DNAfragmentationandlibraryprepin2.5hours*
  • FlexibleDNAsampleinputfrom1ng–1µg*
  • Reducedbiasandmaximizesequencecoverage*
  • PCR-freeworkflows

NEW!KAPADual-IndexedAdapterKitsarenowavailable.FormoreinformationonKAPAAdapterKits,scrolldowntotheOrderingsection,ordownloadtheKAPAAdapterandBeadCalculator.

Downloadour AdapterandBeadCalculator

 

*Dataonfile.
ForResearchUseOnly.Notforuseindiagnosticprocedures.

Workflow

KAPAHyperPlusWorkflow

ProductHighlights

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Tunableandreproduciblefragmentation

  • Adjustlibraryinsertsizesfrom150–800bpbyvaryingfragmentationtime
  • ReproducibleinsertsizesacrossarangeofGCcontentandDNAinputamounts

Industryleadinglibraryyields…

  • Routinelyachieveconversionrates>80%from≥100nginput*
  • SuperiorperformanceacrossarangeofDNAinputamounts*
  • HighlibraryyieldsenablePCR-freeworkflowsfromaslittleas50ngstartingmaterial*
Single nucleotide polymorphism (SNP) detection sensitivity indicates better sequencing quality. Libraries were prepared for capture using the Nimblegen SeqCap EZ HGSC VCRome panel, 50 ng input, standard capture protocol. SNPs were called using LoFreq with default settings and a minimum per-base depth of coverage cut-off of 10. Sensitivity (true positive rate) was calculated as the ratio of true positives divided by the sum of true positives and false negatives. Total SNPs analysed was greater than 25,000. Data on file.
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…Enablesuperiorsequencingresults

  • Higherconversionratesresultsinfeweramplificationcyclesandlowerduplicationrates
  • Detectlow-frequencymutationswithhighconfidenceduetogreaterlibrarydiversityandmoreuniformsequencecoverage
GC bias comparison. GC bias for C. difficile, E. coli, B. pertussis was assessed by calculating the GC content of the reference in 100 bp bins and plotting normalized coverage across these bins for Covaris and Hyper Prep, HyperPlus, NEBNext ® Ultra ™ and Nextera ™ workflows, using Picard CollectGCBiasMetrics. Libraries were prepared from 1 ng of input DNA. In the absence of sequencing bias, all bins would be equally represented, indicated by a horizontal distribution centered on a normalized coverage of 1.0. Distribution of GC content in the genome is indicated by the grey histograms. Data on file.
GC bias comparison. GC bias for C. difficile, E. coli, B. pertussis was assessed by calculating the GC content of the reference in 100 bp bins and plotting normalized coverage across these bins for Covaris and Hyper Prep, HyperPlus, NEBNext ® Ultra ™ and Nextera ™ workflows, using Picard CollectGCBiasMetrics. Libraries were prepared from 1 ng of input DNA. In the absence of sequencing bias, all bins would be equally represented, indicated by a horizontal distribution centered on a normalized coverage of 1.0. Distribution of GC content in the genome is indicated by the grey histograms. Data on file.
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Minimalsequencecoveragebias

  • Lowersequencebiaswhencomparedtotagmentationandotherenzymaticfragmentationmethods*
  • Equivalentperformancetomechanicalshearing*
  • Lessbiasleadstomoreuniformsequencingcoverageandreducedsequencingcosts*

 

*Dataonfile.

RelatedProducts

Areyousequencinglow-input,FFPEorhighqualityDNA? RNA? CheckouttheseKapaNGSproductstoimproveyourworkflowandresults:

Sample QC

KAPAhgDNAQuantificationandQCKit

Library Amplification

KAPALibraryAmplificationKits

Library Quantification

KAPALibraryQuantificationKits

KAPA Stranded mRNA-Seq Kits

KAPAStrandedmRNA-SeqKits

Applications:

Applications
  • WholeGenomeSequencing
  • WholeExomeSequencing
  • TargetedSequencing(custompanels)
  • FFPESampleSequencing

KitSpecificationsandContents/Storage:

KitSpecificationsandContents/Storage

Kitscanbestoredforupto6monthsat-20ºC.

KitscontainKAPAFragEnzyme,KAPAFragBuffer(10X),KAPAFragConditioningSolution,KAPAEndRepair&A-TailingBuffer, KAPAEndRepair&A-TailingEnzyme, KAPALigationBuffer, KAPADNALigase, KAPAHiFiHotStartReadyMixand KAPALibraryAmplificationPrimerMix.

 

 

Specifications

Spec
Description
CompatiblePlatform
lluminaHiSeq®,MiSeq®,NextSeq®
LibraryType
DNA
StartingMaterial
genomicDNA,FFPEDNA,PCRamplicons
InputAmount
1ng–1µg

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