卓越性能,Mobitec巨大芽孢杆菌表达系统

产品名称：卓越性能，Mobitec巨大芽孢杆菌表达系统英文名称：Bacillus megaterium Expression Systems产品型号：kit产品规格：kit产品产地：德国品牌商标：MOBITEC价格：面议元产品详细描述

说明书(bmeg_handbook.pdf)Features: Stable, high-yield protein expression inBacillus megaterium
Ideal for both small and large-scale protein production Tightly regulated and efficiently inducible xylose operon (up to 350-fold) No alkaline proteases activity even up to 5 hours after induction No endotoxins observed Versatile cloning (extended polylinker, additionalBsrG I site*) Versatile production (intracellular or extracellular) Versatile purification (native, 6xHis-tag, Strep-tag, Strep/6xHis double-tag) Removeable tag versions (for TEV or Xa proteases) available Compatible with allB. subtilisvectors

*BsrG Isite is not included in all vectors.

TheB. megateriumexpression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small and large scale.

B. megateriumhas proven to be an excellent alternative host toE. colifor heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

High Protein Yield
As a result, protein yields are exceptionally high even if inexpensive substrates are used. For example, the proteins mutarotase (Mro) and glucose dehydrogenase (Gdh) have been accumulated up to 20% and 30% of the total soluble protein, respectively. Using the tightly regulated xylose operon, the genes were induced 130- to 350-fold without proteolysis.

Versatile System with a Wide Range of Vectors
MoBiTec provides a wide range of useful vectors for theB. megateriumsystem that are adaptable for most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence, a 6xHis-tag, a Strep-tag, and a Strep/6xHis double-tag. Additionally, all vectors contain the tightly regulated and highly inducible xylose operon promoter.

B. megateriumprotoplasts specifically optimized for transformation
The protoplasts supplied by MoBiTec are fromB. megateriumstrain WH320 developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.
For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

Examples:

Selected Proteins successfully over-produced inBacillistrains with ourB. megateriumvectors:

-Galactosidase (LacZ)1 Catabolite control protein (CcpA)2,3 Clostridium difficiletoxin A4 Cobaltochelatase (CbiX)5 Dextransucrase6 Endolevanase (LevB)7 Glucose dehydrogenase (GdhA)1 Heat shock protein (HPr) from PTS (phosphotransferase sugar transport system)8 Human single-chain urokinase-like plasminogen activator (rscuPA)1 Levansucrase9 Mutarotase (Mro)1 Neopullulanase10 Translocation ATPase of the preprotein translocase (SecA)11 Trehalose repressor (TreR)12