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  • 药准字:
  • 主要组成成分:mobitec的巨大芽孢杆菌表达系统
  • 性状:mobitec的巨大芽孢杆菌表达系统
  • 适应症:mobitec的巨大芽孢杆菌表达系统
  • 规格:mobitec的巨大芽孢杆菌表达系统
  • 贮藏:mobitec的巨大芽孢杆菌表达系统
  • 有效期:36
  • 生产企业:德国

hp-vector表达系统megaterium芽孢杆菌
hp-vectorexpressionsystemsforbacillusmegaterium
features:
highperformancevectorswithoptimizedsequence
proteinyieldupto10timesenhancedcomparedtoproteinexpressionwithbasicplasmid
plasmidswithestablishedmcs
encodingc-orn-terminalhis-tagforversatilepurification(native,6xhis-tag)
secretionwithlipaoryochsignalpeptideupto9foldincreased
optimizedhighperformance(hp)vectorsforbacillusmegaterium!
mobitecprovidesawiderangeofusefulvectorsfortheb.megateriumsystemthatareadaptabletomostapplicationsandproteinpurificationmethods.theseincludevectorscarryingasecretionsignalpeptidesequenceanda6xhis-tag.
withournewhp-vectorsyieldsupto10-foldhighercomparedtothebasicplasmidsarepossIBLe.allplasmidshaveestablishedmultiplecloningsites(mcs)forversatilecloning.furThermoreweoffervectorsencodingc-orn-terminalhis-tagsforeasypurification.theproteinsecretionwithlipaoryochsignalpeptidesisuptonine-foldincreased.inductionofproteinexpressionofallvectorsisachievedbythetightlyregulatedandefficientlyinduciblexyloseoperon.
versatilesystemwithawiderangeofvectors
theb.megateriumexpressionsystemprovidesaversatileandeasy-to-handletoolforstableandhigh-yieldproteinproduction,bothsmall-andlarge-scale.
b.megateriumhasproventobeanexcellentalternativehosttoe.coliforheterologousgeneexpression.unlikeotherbacillistrains,proteolyticdegradationbyalkalineproteasesisavoided.inaddition,therearenoendotoxinsfoundinthecellwall.
b.megateriumprotoplastsspecificallyoptimizedfortransformation
theprotoplastssuppliedbymobitecarefromb.megateriumstrainwh320,developedbyprof.dr.w.hillenattheinstituteofmicroBIOLOGyinerlangen,germany.theseprotoplastsarepreparedaccordingtoanoptimizedprotocolresultinginthehighesttransformationefficiencies.
forsecretionvectorsweofferprotoplastsofstrainms941.bothstrainsaremutantsofdsm319,wherewh320isachemicallymutant,whilems941hasadefineddeletioninthegeneofthemajorextracellularproteasenprm.
orderinformation,shippingandstorage
pleaseclickontherespectiveorder#forfurtherdetails.
order#descriptionamount
bmeg02bacillusmegateriumprotoplastsreadyfortransformation(strainwh320;1)
materialissufficientfor4transformationspluscontrolexperiment.5x500µl
bmeg50bacillusmegateriumprotoplasts,strainms9415x500µl
bmeg31pc-his1623hpshuttlevector,c-term.6xhis-tag;lyophilizeddna10μg
bmeg32pn-his-tev1623hpshuttlevector,n-term.his-tag,tev-site10μg
bmeg30p3stop1623hp,shuttlevector,lyophilizeddna10μg
bmeg33psplipa-hp,shuttlevectorwithsignalsequence,lypophilizeddna10μg
bmeg34pspyoch-hp,shuttlevectorwithsignalsequence,lypophilizeddna10µg
shippedatrt,protoplastsshippedondryice
storelyophilizedvectorsat4°c,reconstitutedvectorsat-20°c,protoplastsat-70°c
allvectorsaree.coli/b.megateriumshuttlevectors
pleasenotethatprotoplastsofbacillusmegateriumhavealimitedshelflifeofsomemonthsonly,dependingonthedateofproduction.thereforepurchaseanduseofprotoplastsmustbethoroughlyplanned.

MoBiTec品牌的载体表达系统的产品

 

一、

巨大芽孢杆菌系统

 

 

 

 

 

 

 

Bacillusmegaterium:availabletoacademicandcommercialcustomer;commercialcustomersrequirealicensefromMoBiTec

 

 

载体Vectors:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

BMEG03

BacillusmegateriumvectorpWH1520,lyophilizedDNA

5µg

¥4,900.00

4

RT

 

 

 

BMEG10

Bacillusmegateriumvector,pMM1522,lyophilizedDNA

10µg

¥4,900.00

4

RT

 

 

 

BMEG11

Bacillusmegateriumvector,pMM1525,lyophilizedDNA

10µg

¥4,900.00

4

RT

 

 

 

BMEG12

Bacillusmegateriumvector,pHIS1522,lyophilizedDNA

10µg

¥4,900.00

4

RT

 

 

 

BMEG13

Bacillusmegateriumvector,pHIS1525,lyophilizedDNA

10µg

¥4,900.00

4

RT

 

 

 

BMEG13C

Levansucraseexpressionpositivecontrol,HIS-Tag

10µg

¥1,080.00

4

RT

 

 

 

BMEG14

Bacillusmegateriumvector,pSTREP1525

10µg

¥5,640.00

4

RT

 

 

 

BMEG14C

Levansucraseexpressionpositivecontrol,STREP-Tag

10µg

¥1,080.00

4

RT

 

 

 

BMEG15

BacillusmegateriumvectorpSTREPHIS1525

10µg

¥5,640.00

4

RT

 

 

 

BMEG15C

Levansucraseexpressionpositivecontrol,STREPHIS-Tag

10µg

¥1,080.00

4

RT

 

 

 

BMEG20

BacillusmegateriumvectorpC-His1622

10µg

¥6,840.00

4

RT

 

 

 

BMEG21

BacillusmegateriumvectorpC-Strep1622

10µg

¥8,420.00

4

RT

 

 

 

BMEG22

BacillusmegateriumvectorpN-His-TEV1622

10µg

¥6,840.00

4

RT

 

 

 

BMEG23

BacillusmegateriumvectorpN-Strep-TEV1622

10µg

¥8,420.00

4

RT

 

 

 

BMEG24

BacillusmegateriumvectorpN-StrepXa1622

10µg

¥8,420.00

4

RT

 

 

 

BMEG25

Bacillusmegateriumvector,pS622,lyophilizedDNA.

10µg

¥6,840.00

4

RT

 

 

 

BMEG30

Bacillusmegateriumvectorp3S623hp

10µg

¥19,360.00

4

RT

 

 

 

BMEG31

BacillusmegateriumvectorpC-HIS1623hp

10µg

¥20,420.00

4

RT

 

 

 

BMEG32

BacillusmegateriumvectorpN-HIS-TEV1623hp

10µg

¥20,420.00

4

RT

 

 

 

BMEG33

BacillusmegateriumvectorpSP-LipA-hp

10µg

¥21,260.00

4

RT

 

 

 

BMEG34

BacillusmegateriumvectorpSP-YocH-hp

10µg

¥21,260.00

4

RT

 

 

 

BMEG35

Bacillusmegateriumvectorp3S623-2RBShp

10µg

¥20,000.00

4

RT

 

 

 

BMEG36

BacillusmegateriumvectorpC-STREP1623hp

10µg

¥20,740.00

4

RT

 

 

 

BMEG37

BacillusmegateriumvectorpN-STREP-Xa1623hp

10µg

¥20,940.00

4

RT

 

 

 

BMEG38

BacillusmegateriumvectorpN-STREP-TEV1623hp

10µg

¥20,940.00

4

RT

 

 

 

BMEG10C

pGFP1522,GFPexpressionvector,positivecontrol

10µg

¥1,080.00

4

RT

 

 

 

BMEG39

Bacillusmegateriumvector,pMGBm19,lyophilizedDNA

10µg

¥19,360.00

4

RT

 

 

 

BMEG40C

pGFP1624hp,highperformanceGFPexpressionvector,positivecontrol

10µg

¥9,680.00

4

RT

 

 

 

BMEG45

BacillusmegateriumvectorpPconst1326

10µg

¥11,260.00

4

RT

 

 

 

BMEGT710

BacillusmegateriumpPT7cloningvector,lyophilized

10µg

¥11,260.00

4

RT

 

 

 

BMEGT710C

BacillusmegateriumpPT7-GFPcontrolvector,lyophilized

10µg

¥5,180.00

4

RT

 

 

 

BMEGT711

BacillusmegateriumpPT7-SPlipAsecretionvector

10ug

¥12,320.00

4

RT

 

 

 

 

 

 

 

 

 

 

 

菌株Strains:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

BMEG02

Bacillusmegateriumprotoplasts,strainWH320

5x500µl

¥7,580.00

80

DI

 

 

 

BMEG04

Bacillusmegateriumprotoplasts,strainYYBm1

5x500µl

¥8,720.00

80

DI

 

 

 

BMEG50

Bacillusmegateriumprotoplasts,strainMS941

5x500µl

¥8,440.00

80

DI

 

 

 

 

 

 

 

 

 

 

 

组合Mixed:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

BMEGT701

BacillusmegateriumhighyieldT7geneexpressionkit,includespretransformedprotoplastsBMEGT702(5x500µl),pPT7cloningvectorandpPT7-GFPcontrolvector(vectorslyophilized,10µgeach)

Kit

¥20,780.00

80

DI

 

 

 

BMEGT702

Bacillusmegateriumprotoplasts,strainMS941,pretransformedwithpT7-RNAP

5x500µL

¥12,440.00

80

DI

 

 

 

 

 

 

 

 

 

二、

枯草芽孢杆菌 non-SURE

 

 

 

 

 

 

Bacillussubtilisnon-SURE®:availabletoacademicandcommercialcustomer,commercialcustomersrequirealicensefromMoBiTec

 

 

 

 

 

 

 

 

 

 

 

载体Vectors:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

PBS001

pHT01vector,lyophilizedplasmidDNA

10µg

¥8,040.00

4

RT

 

 

 

PBS001C

pHT01-bgaBcontrolvector,lyophilizedplasmidDNA,availableonlyincombinationwithregularvector

10µg

¥3,100.00

4

RT

 

 

 

PBS002

pHT43vector,lyophilizedplasmidDNA

10µg

¥8,040.00

4

RT

 

 

 

PBS002C

pHT43-amyQcontrolvector,lyophilizedplasmidDNA,availableonlyincombinationwithregularvector

10µg

¥3,100.00

4

RT

 

 

 

PBS003

pHT08vector,lyophilizedplasmidDNA

10µg

¥8,040.00

4

RT

 

 

 

PBS004

pHT09vector,lyophilizedplasmidDNA

10µg

¥8,040.00

4

RT

 

 

 

PBS005

pHT10vector,lyophilizedplasmidDNA

10µg

¥8,040.00

4

RT

 

 

 

PBS005C

pHT10-gfp+controlvector,lyophilizedplasmidDNA,availableonlyincombinationwithregularvector

10µg

¥3,100.00

4

RT

 

 

 

PBS013

pHT253(pHT-Pgrac100-His-Tag-MCS)

10µg

¥9,620.00

4

RT

 

 

 

PBS014

pHT254(pHT-Pgrac100-MCS-His-Tag)

10µg

¥9,620.00

4

RT

 

 

 

PBS015

pHT255(pHT-Pgrac100-MCS-STREP-Tag)

10µg

¥9,620.00

4

RT

 

 

 

 

 

 

 

 

 

 

 

菌株Strains:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

PBS020

Bacillussubtilisstrain1012wt

1mL

¥8,460.00

80

DI

 

 

 

PBS021

Bacillussubtilisstrain168Marburg

1mL

¥6,360.00

80

DI

 

 

 

PBS022

BacillussubtilisstrainWB800N(forsecretionvectors)

1mL

¥11,140.00

80

DI

 

 

 

PBS026

BacillussubtilisstrainAS1

1mL

¥9,300.00

80

DI

 

 

 

 

 

 

 

 

 

三、

枯草芽孢杆菌 SURE

 

 

 

 

 

 

BacillussubtilisSURE®:availabletoacademiccustomersonly;commercialcustomerrequiretonegotiatewiththelicenserNIZO(Netherlands)

 

 

 

 

 

 

 

 

 

 

 

载体Vectors:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

PBS031

pNZ8901vector,lyophilizedDNA

10µg

¥9,620.00

4

RT

 

 

 

PBS032

pNZ8902vector,lyophilizedDNA

10µg

¥9,620.00

4

RT

 

 

 

PBS033

pNZ8910vector,lyophilizedDNA

10µg

¥9,620.00

4

RT

 

 

 

PBS034

pNZ8911vector,lyophilizedDNA

10µg

¥9,620.00

4

RT

 

 

 

 

 

 

 

 

 

 

 

菌株Strains:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

PBS023

BacillussubtilisstrainNZ8963

1ml

¥9,620.00

80

DI

 

 

 

PBS024

BacillussubtilisstrainNZ8900

1ml

¥9,620.00

80

DI

 

 

 

PBS025

BacillussubtilisstrainNZ8901

1ml

¥6,360.00

80

DI

 

 

 

 

 

 

 

 

 

四、

乳酸乳球菌的载体和菌株

 

 

 

 

 

 

Lactococcuslactisvectorsandstrains: availabletoacademiccustomersonly;commercialcustomerrequiretonegotiatewiththelicenserNIZO(Netherlands)

 

 

 

 

 

 

 

 

 

 

 

载体Vectors:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

VS-ELV00100-01

NICE®pNZ8008ReferenceplasmidwithgusAgene

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00200-01

NICE®pNZ8148Lactococcuslactisexpressionvector,NcoIsite

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00250-01

NICE®pNZ8150Lactococcuslactisexpressionvector,ScaIsite

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00300-01

NICE®pNZ8149Lactococcuslactisexpressionvector,foodgrade(lacF),NcoIsite

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00310-01

NICE®pNZ8151Lactococcuslactisexpressionvector,foodgrade(lacF),Scalsite

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00400-01

NICE®pNZ8152Lactococcuslactisexpressionvector,foodgrade(alr),Scalsite

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00500-01

NICE®pNZ9530LactococcuslactisnisRnisKvector,inStrainNZ9000

1ml

¥5,340.00

80

DI

 

 

 

VS-ELV00600-01

NICE®pNZ8120Lactococcuslactissecretionvector(SPPrtP/NaeI)

10µg

¥11,120.00

4

RT

 

 

 

VS-ELV00650-01

NICE®pNZ8121Lactococcuslactissecretionvector(SPPrtP/EcoRV)

10µg

¥11,120.00

4

RT

 

 

 

VS-ELV00700-01

NICE®pNZ8122Lactococcuslactissecretionvector(SPSlpA/NruI)

10µg

¥11,120.00

4

RT

 

 

 

VS-ELV00750-01

NICE®pNZ8123Lactococcuslactissecretionvector(SPUSP45/NaeI)

10µg

¥11,120.00

4

RT

 

 

 

VS-ELV00800-01

NICE®pNZ8124Lactococcuslactissecretionvector(SPUsp45/EcoRV)

10µg

¥11,120.00

4

RT

 

 

 

VS-ELV01100

pNZ124Lactococcuslactiscloningvector

10µg

¥8,360.00

4

RT

 

 

 

VS-ELV01150

pNZ2105Lactococcuslactiscloningvector,foodgrade

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV01200

pNZ2103Lactococcuslactisconst.vector,lacA

10µg

¥8,560.00

4

RT

 

 

 

VS-ELV01250

pNZ7021Lactococcuslactisconst.vector,pepN

10µg

¥8,560.00

4

RT

 

 

 

VS-ELV01300

pNZ2122Lactococcuslactisconst.vector,mcsX-H,foodgrade

10µg

¥8,960.00

4

RT

 

 

 

VS-ELV01350

pNZ2123Lactococcuslactisconst.vector,mcsH-X,foodgrade

10µg

¥8,960.00

4

RT

 

 

 

VS-ELV01400

pNZ2125Lactococcuslactisconst.vector,foodgrade,(alr)

10µg

¥8,960.00

4

RT

 

 

 

VS-ELV01450

pNZ7025Lactococcuslactisconst.vector,pepN,foodgrade,(alr)

10µg

¥8,960.00

4

RT

 

 

 

 

 

 

 

 

 

 

 

菌株Strains:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

VS-ELS10710-01

MC1061ChemicallyCompetent Escherichiacoli

5x100µl

¥7,960.00

80

DI

 

 

 

VS-ELS01363

LactococcuslactisexpressionstrainMG1363

1ml

¥5,340.00

80

DI

 

 

 

VS-ELS03000

LactococcuslactisexpressionStrainNZ3000,foodgrade

1ml

¥5,680.00

80

DI

 

 

 

VS-ELS03900-01

NICE®LactococcuslactisexpressionStrainNZ3900,foodgrade,(lacF)

1ml

¥5,340.00

80

DI

 

 

 

VS-ELS03910-01

NICE®LactococcuslactisexpressionStrainNZ3910,foodgrade,(lacF)

1ml

¥5,780.00

80

DI

 

 

 

VS-ELS09000-01

NICE®LactococcuslactisexpressionStrainNZ9000

1ml

¥5,340.00

80

DI

 

 

 

VS-ELS09100

NICE®LactococcuslactisexpressionStrainNZ9100

1ml

¥5,780.00

80

DI

 

 

 

VS-ELS09130-01

NICE®LactococcuslactisexpressionStrainNZ9130,foodgrade,(alr)

1ml

¥6,960.00

80

DI

 

 

 

VS-ELS10610-01

NICE®E.colihostStrainMC1061

1ml

¥5,540.00

80

DI

 

 

 

VS-ELS01330-01

NICE®LactococcuslactisexpressionStrainNZ1330,foodgrade,(alr)

1ml

¥6,960.00

80

DI

 

 

 

 

 

 

 

 

 

 

 

组合Mixed:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

VS-ELV00500-01

NICE®pNZ9530LactococcuslactisnisRnisKvector,inStrainNZ9000

1ml

¥5,340.00

80

DI

 

 

 

 

 


“mobitec酵母双杂交/单杂交系统”其他说明

酵母双(单)杂交系统:Y2H膜蛋白系统;Grow""n""GlowGFP酵母单(双)杂交系统;Grow´n´GlowACE1酵母双杂交系统;Y2H膜蛋白系统CDNA文库;酵母载体;酵母菌;酵母转化和质粒提取;β-半乳糖苷酶活性检测试剂盒;
噬菌体展示:pSKAN噬菌体展示系统;Anti-plll(g3p)抗体;抗体;pSKAN8载体;pMAMPF3-PSTI4载体;LamBDaPS噬菌体裂解物;Anti-hPSTI 
基因文库:Y2HcDNA文库;LambdaPS基因文库;

公司旨在为客户提供高质量的实验外包服务(CRO服务),包括实验方案设计,常规基因工程实验操作,重组蛋白表达与纯化,重组抗体制备,天然蛋白分离与纯化,病毒分离培养等免疫学相关技术服务;同时我们也为工业客户提供部分IVD级别抗体和抗原原料。核心平台:---噬菌体抗体展示技术平台苏州蚂蚁淘生物科技有限公司利用自有的噬菌体(M13,T7噬菌体)展示技术平台,能够为客户提供极具性价比的重组Vhh抗体(纳米抗体/驼类纳米抗体)制备方案,包括动物免疫,文库建立,文库筛选以及重组抗体的体外表达等。---抗体工程技术平台卡梅德生物科技的抗体工程平台包含单克隆抗体制备服务平台,多克隆抗体制备服务平台,抗体测序与改造平台。单克隆抗体制备平台:基于杂交瘤技术的小鼠单抗技术平台,最快能够在2-3个月获得高亲和力与高特异性的单克隆抗体,同时基于噬菌体展示技术,我司能够制备多物种的重组单克隆抗体,包括小鼠,兔子,骆驼,羊驼以及人单抗。多克隆抗体制备服务平台:能够为客户提供兔多抗,羊多抗,骆驼类多抗,牛多抗等多物种的抗体制备服务,根据不同的抗原类型,来自蚂蚁淘生物的资深抗体专家会为您量身定做适合您需求的方案,以满足您的项目要求并节省您的宝贵时间。抗体一级序列测序平台:为满足大量客户对抗体基因序列与氨基酸序列信息的需求,我们为客户提供DeNovo抗体全长氨基酸序列解析和N端测序服务;同时我们设计积累多达80种杂交瘤抗体基因测序引物。抗体改造技术平台:蚂蚁淘生物技术的抗体改造平台包括嵌合抗体制备,抗体亲和力成熟以及抗体人源化服务。目前我们已经成功制备十几例嵌合抗体与人源化抗体,均成功交付。---蛋白表达与纯化服务技术平台蚂蚁淘生物科技目前拥有四大蛋白表达平台,能为客户提供全面且优质的蛋白表达技术服务。哺乳动物表达平台:目前公司拥有FreestyleCHO,ExpiCHO,CHO-DG44,HEK293F,Vero等细胞系用于蛋白表达制备。公司秉承质量优先原则,全部统一采用Gibco细胞培养基和相关转染配套试剂,以保证每个批次的哺乳动物细胞蛋白表达产品高质量、高表达水平的交付。同时公司拥有的表达载体体系,远高于市面常用蛋白表达载体,能够为客户提供高纯度蛋白。昆虫细胞表达平台:目前公司常采用的昆虫表达细胞为SF9,配套使用的是来自Gibco的培养基,只...


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