公司旨在为客户提供高质量的实验外包服务(CRO服务),包括实验方案设计,常规基因工程实验操作,重组蛋白表达与纯化,重组抗体制备,天然蛋白分离与纯化,病毒分离培养等免疫学相关技术服务;同时我们也为工业客户提供部分IVD级别抗体和抗原原料。核心平台:---噬菌体抗体展示技术平台苏州蚂蚁淘生物科技有限公司利用自有的噬菌体(M13,T7噬菌体)展示技术平台,能够为客户提供极具性价比的重组Vhh抗体(纳米抗体/驼类纳米抗体)制备方案,包括动物免疫,文库建立,文库筛选以及重组抗体的体外表达等。---抗体工程技术平台卡梅德生物科技的抗体工程平台包含单克隆抗体制备服务平台,多克隆抗体制备服务平台,抗体测序与改造平台。单克隆抗体制备平台:基于杂交瘤技术的小鼠单抗技术平台,最快能够在2-3个月获得高亲和力与高特异性的单克隆抗体,同时基于噬菌体展示技术,我司能够制备多物种的重组单克隆抗体,包括小鼠,兔子,骆驼,羊驼以及人单抗。多克隆抗体制备服务平台:能够为客户提供兔多抗,羊多抗,骆驼类多抗,牛多抗等多物种的抗体制备服务,根据不同的抗原类型,来自蚂蚁淘生物的资深抗体专家会为您量身定做适合您需求的方案,以满足您的项目要求并节省您的宝贵时间。抗体一级序列测序平台:为满足大量客户对抗体基因序列与氨基酸序列信息的需求,我们为客户提供DeNovo抗体全长氨基酸序列解析和N端测序服务;同时我们设计积累多达80种杂交瘤抗体基因测序引物。抗体改造技术平台:蚂蚁淘生物技术的抗体改造平台包括嵌合抗体制备,抗体亲和力成熟以及抗体人源化服务。目前我们已经成功制备十几例嵌合抗体与人源化抗体,均成功交付。---蛋白表达与纯化服务技术平台蚂蚁淘生物科技目前拥有四大蛋白表达平台,能为客户提供全面且优质的蛋白表达技术服务。哺乳动物表达平台:目前公司拥有FreestyleCHO,ExpiCHO,CHO-DG44,HEK293F,Vero等细胞系用于蛋白表达制备。公司秉承质量优先原则,全部统一采用Gibco细胞培养基和相关转染配套试剂,以保证每个批次的哺乳动物细胞蛋白表达产品高质量、高表达水平的交付。同时公司拥有的表达载体体系,远高于市面常用蛋白表达载体,能够为客户提供高纯度蛋白。昆虫细胞表达平台:目前公司常采用的昆虫表达细胞为SF9,配套使用的是来自Gibco的培养基,只...
性能参数
产品名称: 卓越性能,Mobitec巨大芽孢杆菌表达系统
英文名称: BacillusmegateriumExpressionSystems
产品型号: kit
产品规格: kit
产品产地: 德国
品牌商标: MOBITEC
价 格: 面议元
产品详细描述
说明书(
bmeg_handbook.pdf)
Features:
- Stable,high-yieldproteinexpressionin Bacillusmegaterium
- Idealforbothsmallandlarge-scaleproteinproduction
- TightlyregulatedandefficientlyinducIBLexyloseoperon(upto350-fold)
- Noalkalineproteasesactivityevenupto5hoursafterinduction
- Noendotoxinsobserved
- Versatilecloning(extendedpolylinker,additional BsrGIsite*)
- Versatileproduction(intracellularorextracellular)
- Versatilepurification(native,6xHis-tag,Strep-tag,Strep/6xHisdouble-tag)
- Removeabletagversions(forTEVorXaproteases)available

- Compatiblewithall B.subtilis vectors
*BsrGI siteisnotincludedinallvectors.
The B.megaterium expressionsystemprovidesaversatileandeasy-to-handletoolforstableandhigh-yieldproteinproduction,bothsmallandlargescale.
B.megaterium hasproventobeanexcellentalternativehostto E.coli forheterologousgeneexpression.Unlikeotherbacillistrains,proteolyticdegradationbyalkalineproteasesisavoided.Inaddition,therearenoendotoxinsfoundinthecellwall.
HighProteinYield
Asaresult,proteinyieldsareexceptionallyhighevenifinexpensivesubstratesareused.Forexample,theproteinsmutarotase(Mro)andglucosedehydrogenase(Gdh)havebeenaccumulatedupto20%and30%ofthetotalsolubleprotein,respectively.Usingthetightlyregulatedxyloseoperon,thegeneswereinduced130-to350-foldwithoutproteolysis.
VersatileSystemwithaWideRangeofVectors
MoBiTecprovidesawiderangeofusefulvectorsforthe B.megaterium systemthatareadaptableformostapplicationsandproteinpurificationmethods.Theseincludevectorscarryingasecretionsignalpeptidesequence,a6xHis-tag,aStrep-tag,andaStrep/6xHisdouble-tag.Additionally,allvectorscontainthetightlyregulatedandhighlyinduciblexyloseoperonpromoter.
B.megaterium protoplastsspecificallyoptimizedfortransformation
TheprotoplastssuppliedbyMoBiTecarefrom B.megaterium strainWH320developedbyProf.Dr.W.HillenattheInstituteofMicroBIOLOGyinErlangen,Germany.Theseprotoplastsarepreparedaccordingtoanoptimizedprotocolresultinginthehighesttransformationefficiencies.
ForsecretionvectorsweofferprotoplastsofstrainMS941.BothstrainsaremutantsofDSM319,whereWH320isachemicallymutant,whileMS941hasadefineddeletioninthegeneofthemajorextracellularproteaseNprM.
Examples:
SelectedProteinssuccessfullyover-producedin Bacilli strainswithour B.megaterium vectors:
- β-Galactosidase(LacZ)1
- Catabolitecontrolprotein(CcpA)2,3
- Clostridiumdifficile toxinA4
- Cobaltochelatase(CbiX)5
- Dextransucrase6
- Endolevanase(LevB)7
- Glucosedehydrogenase(GdhA)1
- Heatshockprotein(HPr)fromPTS(phosphotransferasesugartransportsystem)8
- Humansingle-chainurokinase-likeplasminogenactivator(rscuPA)1
- Levansucrase9
- Mutarotase(Mro)1
- Neopullulanase10
- TranslocationATPaseofthepreproteintranslocase(SecA)11
- Trehaloserepressor(TreR)12
- 药准字:
- 主要组成成分:mobitec的巨大芽孢杆菌表达系统
- 性状:mobitec的巨大芽孢杆菌表达系统
- 适应症:mobitec的巨大芽孢杆菌表达系统
- 规格:mobitec的巨大芽孢杆菌表达系统
- 贮藏:mobitec的巨大芽孢杆菌表达系统
- 有效期:36
- 生产企业:德国
hp-vector表达系统megaterium芽孢杆菌hp-vectorexpressionsystemsforbacillusmegateriumfeatures:highperformancevectorswithoptimizedsequenceproteinyieldupto10timesenhancedcomparedtoproteinexpressionwithbasicplasmidplasmidswithestablishedmcsencodingc-orn-terminalhis-tagforversatilepurification(native,6xhis-tag)secretionwithlipaoryochsignalpeptideupto9foldincreasedoptimizedhighperformance(hp)vectorsforbacillusmegaterium!mobitecprovidesawiderangeofusefulvectorsfortheb.megateriumsystemthatareadaptabletomostapplicationsandproteinpurificationmethods.theseincludevectorscarryingasecretionsignalpeptidesequenceanda6xhis-tag.withournewhp-vectorsyieldsupto10-foldhighercomparedtothebasicplasmidsarepossible.allplasmidshaveestablishedmultiplecloningsites(mcs)forversatilecloning.furThermoreweoffervectorsencodingc-orn-terminalhis-tagsforeasypurification.theproteinsecretionwithlipaoryochsignalpeptidesisuptonine-foldincreased.inductionofproteinexpressionofallvectorsisachievedbythetightlyregulatedandefficientlyinduciblexyloseoperon.versatilesystemwithawiderangeofvectorstheb.megateriumexpressionsystemprovidesaversatileandeasy-to-handletoolforstableandhigh-yieldproteinproduction,bothsmall-andlarge-scale.b.megateriumhasproventobeanexcellentalternativehosttoe.coliforheterologousgeneexpression.unlikeotherbacillistrains,proteolyticdegradationbyalkalineproteasesisavoided.inaddition,therearenoendotoxinsfoundinthecellwall.b.megateriumprotoplastsspecificallyoptimizedfortransformationtheprotoplastssuppliedbymobitecarefromb.megateriumstrainwh320,developedbyprof.dr.w.hillenattheinstituteofmicrobiologyinerlangen,germany.theseprotoplastsarepreparedaccordingtoanoptimizedprotocolresultinginthehighesttransformationefficiencies.forsecretionvectorsweofferprotoplastsofstrainms941.bothstrainsaremutantsofdsm319,wherewh320isachemicallymutant,whilems941hasadefineddeletioninthegeneofthemajorextracellularproteasenprm.orderinformation,shippingandstoragepleaseclickontherespectiveorder#forfurtherdetails.order#descriptionamountbmeg02bacillusmegateriumprotoplastsreadyfortransformation(strainwh320;1)materialissufficientfor4transformationspluscontrolexperiment.5x500µlbmeg50bacillusmegateriumprotoplasts,strainms9415x500µlbmeg31pc-his1623hpshuttlevector,c-term.6xhis-tag;lyophilizeddna10μgbmeg32pn-his-tev1623hpshuttlevector,n-term.his-tag,tev-site10μgbmeg30p3stop1623hp,shuttlevector,lyophilizeddna10μgbmeg33psplipa-hp,shuttlevectorwithsignalsequence,lypophilizeddna10μgbmeg34pspyoch-hp,shuttlevectorwithsignalsequence,lypophilizeddna10µgshippedatrt,protoplastsshippedondryicestorelyophilizedvectorsat4°c,reconstitutedvectorsat-20°c,protoplastsat-70°callvectorsaree.coli/b.megateriumshuttlevectorspleasenotethatprotoplastsofbacillusmegateriumhavealimitedshelflifeofsomemonthsonly,dependingonthedateofproduction.thereforepurchaseanduseofprotoplastsmustbethoroughlyplanned.
成核剂,播种和定相试剂盒。
Polyvalan的CrystallophoreNo1-一种发光的complex络合物,是有效的核清洁剂,可提供明显的同构和异常定相能力。唯yi可以一次解决三个问题的化合物。
˚Forming:种植能够产生高质量的衍射数据的晶体
˚Finding:识别晶体滴快速,准确地
博士 ASING:在F“〜30层的电子对铽3+大号III边缘在1.6或7.5千电子伏 Naomi的Nucleants-Naomi的Nucleant促进了14种蛋白质的结晶,这是任何单个nucleant报道的zui高数量。这些蛋白质中的许多已被证明难以结晶,而其中一些蛋白质,包括膜蛋白质,仅在NaomiNucleant存在的情况下才结晶。
微种子珠 -微种子珠用于生成微种子-已“粉碎”成碎片的微观晶体。
微晶播种的效果– IspD蛋白在微晶簇在液滴边缘成簇生长之前(左)和微晶种之后晶体分别生长(右)。(图片由TereseBergfors提供)。
含有3个碘原子的分子排列在等边三角形中,可用于建立SAD和SIRAS实验的实验阶段。
产品编号 产品名称 单位大小 单价 数量
MD2-82 1号晶体电泳仪(小瓶,0.3mg) 0.3毫克 MOLECULARDIMENSIONS 1
MD2-80 1号晶体电泳仪(大瓶,0.6mg) 0.6毫克 MOLECULARDIMENSIONS 1
MD2-81 1号晶体荧光体(5个大的0.6mg小瓶)5x0.6毫克 MOLECULARDIMENSIONS 1
MD2-11 I3C相位套件1套 MOLECULARDIMENSIONS 1
MD2-07 娜奥米的努克利特 1个 MOLECULARDIMENSIONS 1
MD2-14 微种子珠(2.381毫米) 1x24珠 MOLECULARDIMENSIONS 1
MD9-25 细镊子 1个 MOLECULARDIMENSIONS 1
苏州蚂蚁淘生物科技有限公司是专业从事生物技术产品销售的高科技企业,是国内生物试剂、仪器、消耗品领域的知名企业之一。蚂蚁淘公司自成立以来,连续2年高速成长。公司的发展目标是建成专业从事生物技术产品销售、生产与开发的一流公司。