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银川MoBiTec试剂产品信息 MoBiTec ACE1双杂交系统


  • 药准字:
  • 主要组成成分:mobitec的巨大芽孢杆菌表达系统
  • 性状:mobitec的巨大芽孢杆菌表达系统
  • 适应症:mobitec的巨大芽孢杆菌表达系统
  • 规格:mobitec的巨大芽孢杆菌表达系统
  • 贮藏:mobitec的巨大芽孢杆菌表达系统
  • 有效期:36
  • 生产企业:德国

hp-vector表达系统megaterium芽孢杆菌
hp-vectorexpressionsystemsforbacillusmegaterium
features:
highperformancevectorswithoptimizedsequence
proteinyieldupto10timesenhancedcomparedtoproteinexpressionwithbasicplasmid
plasmidswithestablishedmcs
encodingc-orn-terminalhis-tagforversatilepurification(native,6xhis-tag)
secretionwithlipaoryochsignalpeptideupto9foldincreased
optimizedhighperformance(hp)vectorsforbacillusmegaterium!
mobitecprovidesawiderangeofusefulvectorsfortheb.megateriumsystemthatareadaptabletomostapplicationsandproteinpurificationmethods.theseincludevectorscarryingasecretionsignalpeptidesequenceanda6xhis-tag.
withournewhp-vectorsyieldsupto10-foldhighercomparedtothebasicplasmidsarepossible.allplasmidshaveestablishedmultiplecloningsites(mcs)forversatilecloning.furthermoreweoffervectorsencodingc-orn-terminalhis-tagsforeasypurification.theproteinsecretionwithlipaoryochsignalpeptidesisuptonine-foldincreased.inductionofproteinexpressionofallvectorsisachievedbythetightlyregulatedandefficientlyinduciblexyloseoperon.
versatilesystemwithawiderangeofvectors
theb.megateriumexpressionsystemprovidesaversatileandeasy-to-handletoolforstableandhigh-yieldproteinproduction,bothsmall-andlarge-scale.
b.megateriumhasproventobeanexcellentalternativehosttoe.coliforheterologousgeneexpression.unlikeotherbacillistrains,proteolyticdegradationbyalkalineproteasesisavoided.inaddition,therearenoendotoxinsfoundinthecellwall.
b.megateriumprotoplastsspecificallyoptimizedfortransformation
theprotoplastssuppliedbymobitecarefromb.megateriumstrainwh320,developedbyprof.dr.w.hillenattheinstituteofmicrobiologyinerlangen,germany.theseprotoplastsarepreparedaccordingtoanoptimizedprotocolresultinginthehighesttransformationefficiencies.
forsecretionvectorsweofferprotoplastsofstrainms941.bothstrainsaremutantsofdsm319,wherewh320isachemicallymutant,whilems941hasadefineddeletioninthegeneofthemajorextracellularproteasenprm.
orderinformation,shippingandstorage
pleaseclickontherespectiveorder#forfurtherdetails.
order#descriptionamount
bmeg02bacillusmegateriumprotoplastsreadyfortransformation(strainwh320;1)
materialissufficientfor4transformationspluscontrolexperiment.5x500µl
bmeg50bacillusmegateriumprotoplasts,strainms9415x500µl
bmeg31pc-his1623hpshuttlevector,c-term.6xhis-tag;lyophilizeddna10μg
bmeg32pn-his-tev1623hpshuttlevector,n-term.his-tag,tev-site10μg
bmeg30p3stop1623hp,shuttlevector,lyophilizeddna10μg
bmeg33psplipa-hp,shuttlevectorwithsignalsequence,lypophilizeddna10μg
bmeg34pspyoch-hp,shuttlevectorwithsignalsequence,lypophilizeddna10µg
shippedatrt,protoplastsshippedondryice
storelyophilizedvectorsat4°c,reconstitutedvectorsat-20°c,protoplastsat-70°c
allvectorsaree.coli/b.megateriumshuttlevectors
pleasenotethatprotoplastsofbacillusmegateriumhavealimitedshelflifeofsomemonthsonly,dependingonthedateofproduction.thereforepurchaseanduseofprotoplastsmustbethoroughlyplanned.

MoBiTec品牌的载体表达系统的产品

 

一、

巨大芽孢杆菌系统

 

 

 

 

 

 

 

Bacillusmegaterium:availabletoacademicandcommercialcustomer;commercialcustomersrequirealicensefromMoBiTec

 

 

载体Vectors:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

BMEG03

BacillusmegateriumvectorpWH1520,lyophilizedDNA

5µg

¥4,900.00

4

RT

 

 

 

BMEG10

Bacillusmegateriumvector,pMM1522,lyophilizedDNA

10µg

¥4,900.00

4

RT

 

 

 

BMEG11

Bacillusmegateriumvector,pMM1525,lyophilizedDNA

10µg

¥4,900.00

4

RT

 

 

 

BMEG12

Bacillusmegateriumvector,pHIS1522,lyophilizedDNA

10µg

¥4,900.00

4

RT

 

 

 

BMEG13

Bacillusmegateriumvector,pHIS1525,lyophilizedDNA

10µg

¥4,900.00

4

RT

 

 

 

BMEG13C

Levansucraseexpressionpositivecontrol,HIS-Tag

10µg

¥1,080.00

4

RT

 

 

 

BMEG14

Bacillusmegateriumvector,pSTREP1525

10µg

¥5,640.00

4

RT

 

 

 

BMEG14C

Levansucraseexpressionpositivecontrol,STREP-Tag

10µg

¥1,080.00

4

RT

 

 

 

BMEG15

BacillusmegateriumvectorpSTREPHIS1525

10µg

¥5,640.00

4

RT

 

 

 

BMEG15C

Levansucraseexpressionpositivecontrol,STREPHIS-Tag

10µg

¥1,080.00

4

RT

 

 

 

BMEG20

BacillusmegateriumvectorpC-His1622

10µg

¥6,840.00

4

RT

 

 

 

BMEG21

BacillusmegateriumvectorpC-Strep1622

10µg

¥8,420.00

4

RT

 

 

 

BMEG22

BacillusmegateriumvectorpN-His-TEV1622

10µg

¥6,840.00

4

RT

 

 

 

BMEG23

BacillusmegateriumvectorpN-Strep-TEV1622

10µg

¥8,420.00

4

RT

 

 

 

BMEG24

BacillusmegateriumvectorpN-StrepXa1622

10µg

¥8,420.00

4

RT

 

 

 

BMEG25

Bacillusmegateriumvector,pS622,lyophilizedDNA.

10µg

¥6,840.00

4

RT

 

 

 

BMEG30

Bacillusmegateriumvectorp3S623hp

10µg

¥19,360.00

4

RT

 

 

 

BMEG31

BacillusmegateriumvectorpC-HIS1623hp

10µg

¥20,420.00

4

RT

 

 

 

BMEG32

BacillusmegateriumvectorpN-HIS-TEV1623hp

10µg

¥20,420.00

4

RT

 

 

 

BMEG33

BacillusmegateriumvectorpSP-LipA-hp

10µg

¥21,260.00

4

RT

 

 

 

BMEG34

BacillusmegateriumvectorpSP-YocH-hp

10µg

¥21,260.00

4

RT

 

 

 

BMEG35

Bacillusmegateriumvectorp3S623-2RBShp

10µg

¥20,000.00

4

RT

 

 

 

BMEG36

BacillusmegateriumvectorpC-STREP1623hp

10µg

¥20,740.00

4

RT

 

 

 

BMEG37

BacillusmegateriumvectorpN-STREP-Xa1623hp

10µg

¥20,940.00

4

RT

 

 

 

BMEG38

BacillusmegateriumvectorpN-STREP-TEV1623hp

10µg

¥20,940.00

4

RT

 

 

 

BMEG10C

pGFP1522,GFPexpressionvector,positivecontrol

10µg

¥1,080.00

4

RT

 

 

 

BMEG39

Bacillusmegateriumvector,pMGBm19,lyophilizedDNA

10µg

¥19,360.00

4

RT

 

 

 

BMEG40C

pGFP1624hp,highperformanceGFPexpressionvector,positivecontrol

10µg

¥9,680.00

4

RT

 

 

 

BMEG45

BacillusmegateriumvectorpPconst1326

10µg

¥11,260.00

4

RT

 

 

 

BMEGT710

BacillusmegateriumpPT7cloningvector,lyophilized

10µg

¥11,260.00

4

RT

 

 

 

BMEGT710C

BacillusmegateriumpPT7-GFPcontrolvector,lyophilized

10µg

¥5,180.00

4

RT

 

 

 

BMEGT711

BacillusmegateriumpPT7-SPlipAsecretionvector

10ug

¥12,320.00

4

RT

 

 

 

 

 

 

 

 

 

 

 

菌株Strains:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

BMEG02

Bacillusmegateriumprotoplasts,strainWH320

5x500µl

¥7,580.00

80

DI

 

 

 

BMEG04

Bacillusmegateriumprotoplasts,strainYYBm1

5x500µl

¥8,720.00

80

DI

 

 

 

BMEG50

Bacillusmegateriumprotoplasts,strainMS941

5x500µl

¥8,440.00

80

DI

 

 

 

 

 

 

 

 

 

 

 

组合Mixed:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

BMEGT701

BacillusmegateriumhighyieldT7geneexpressionkit,includespretransformedprotoplastsBMEGT702(5x500µl),pPT7cloningvectorandpPT7-GFPcontrolvector(vectorslyophilized,10µgeach)

Kit

¥20,780.00

80

DI

 

 

 

BMEGT702

Bacillusmegateriumprotoplasts,strainMS941,pretransformedwithpT7-RNAP

5x500µL

¥12,440.00

80

DI

 

 

 

 

 

 

 

 

 

二、

枯草芽孢杆菌 non-SURE

 

 

 

 

 

 

Bacillussubtilisnon-SURE®:availabletoacademicandcommercialcustomer,commercialcustomersrequirealicensefromMoBiTec

 

 

 

 

 

 

 

 

 

 

 

载体Vectors:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

PBS001

pHT01vector,lyophilizedplasmidDNA

10µg

¥8,040.00

4

RT

 

 

 

PBS001C

pHT01-bgaBcontrolvector,lyophilizedplasmidDNA,availableonlyincombinationwithregularvector

10µg

¥3,100.00

4

RT

 

 

 

PBS002

pHT43vector,lyophilizedplasmidDNA

10µg

¥8,040.00

4

RT

 

 

 

PBS002C

pHT43-amyQcontrolvector,lyophilizedplasmidDNA,availableonlyincombinationwithregularvector

10µg

¥3,100.00

4

RT

 

 

 

PBS003

pHT08vector,lyophilizedplasmidDNA

10µg

¥8,040.00

4

RT

 

 

 

PBS004

pHT09vector,lyophilizedplasmidDNA

10µg

¥8,040.00

4

RT

 

 

 

PBS005

pHT10vector,lyophilizedplasmidDNA

10µg

¥8,040.00

4

RT

 

 

 

PBS005C

pHT10-gfp+controlvector,lyophilizedplasmidDNA,availableonlyincombinationwithregularvector

10µg

¥3,100.00

4

RT

 

 

 

PBS013

pHT253(pHT-Pgrac100-His-Tag-MCS)

10µg

¥9,620.00

4

RT

 

 

 

PBS014

pHT254(pHT-Pgrac100-MCS-His-Tag)

10µg

¥9,620.00

4

RT

 

 

 

PBS015

pHT255(pHT-Pgrac100-MCS-STREP-Tag)

10µg

¥9,620.00

4

RT

 

 

 

 

 

 

 

 

 

 

 

菌株Strains:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

PBS020

Bacillussubtilisstrain1012wt

1mL

¥8,460.00

80

DI

 

 

 

PBS021

Bacillussubtilisstrain168Marburg

1mL

¥6,360.00

80

DI

 

 

 

PBS022

BacillussubtilisstrainWB800N(forsecretionvectors)

1mL

¥11,140.00

80

DI

 

 

 

PBS026

BacillussubtilisstrainAS1

1mL

¥9,300.00

80

DI

 

 

 

 

 

 

 

 

 

三、

枯草芽孢杆菌 SURE

 

 

 

 

 

 

BacillussubtilisSURE®:availabletoacademiccustomersonly;commercialcustomerrequiretonegotiatewiththelicenserNIZO(Netherlands)

 

 

 

 

 

 

 

 

 

 

 

载体Vectors:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

PBS031

pNZ8901vector,lyophilizedDNA

10µg

¥9,620.00

4

RT

 

 

 

PBS032

pNZ8902vector,lyophilizedDNA

10µg

¥9,620.00

4

RT

 

 

 

PBS033

pNZ8910vector,lyophilizedDNA

10µg

¥9,620.00

4

RT

 

 

 

PBS034

pNZ8911vector,lyophilizedDNA

10µg

¥9,620.00

4

RT

 

 

 

 

 

 

 

 

 

 

 

菌株Strains:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

PBS023

BacillussubtilisstrainNZ8963

1ml

¥9,620.00

80

DI

 

 

 

PBS024

BacillussubtilisstrainNZ8900

1ml

¥9,620.00

80

DI

 

 

 

PBS025

BacillussubtilisstrainNZ8901

1ml

¥6,360.00

80

DI

 

 

 

 

 

 

 

 

 

四、

乳酸乳球菌的载体和菌株

 

 

 

 

 

 

Lactococcuslactisvectorsandstrains: availabletoacademiccustomersonly;commercialcustomerrequiretonegotiatewiththelicenserNIZO(Netherlands)

 

 

 

 

 

 

 

 

 

 

 

载体Vectors:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

VS-ELV00100-01

NICE®pNZ8008ReferenceplasmidwithgusAgene

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00200-01

NICE®pNZ8148Lactococcuslactisexpressionvector,NcoIsite

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00250-01

NICE®pNZ8150Lactococcuslactisexpressionvector,ScaIsite

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00300-01

NICE®pNZ8149Lactococcuslactisexpressionvector,foodgrade(lacF),NcoIsite

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00310-01

NICE®pNZ8151Lactococcuslactisexpressionvector,foodgrade(lacF),Scalsite

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00400-01

NICE®pNZ8152Lactococcuslactisexpressionvector,foodgrade(alr),Scalsite

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV00500-01

NICE®pNZ9530LactococcuslactisnisRnisKvector,inStrainNZ9000

1ml

¥5,340.00

80

DI

 

 

 

VS-ELV00600-01

NICE®pNZ8120Lactococcuslactissecretionvector(SPPrtP/NaeI)

10µg

¥11,120.00

4

RT

 

 

 

VS-ELV00650-01

NICE®pNZ8121Lactococcuslactissecretionvector(SPPrtP/EcoRV)

10µg

¥11,120.00

4

RT

 

 

 

VS-ELV00700-01

NICE®pNZ8122Lactococcuslactissecretionvector(SPSlpA/NruI)

10µg

¥11,120.00

4

RT

 

 

 

VS-ELV00750-01

NICE®pNZ8123Lactococcuslactissecretionvector(SPUsp45/NaeI)

10µg

¥11,120.00

4

RT

 

 

 

VS-ELV00800-01

NICE®pNZ8124Lactococcuslactissecretionvector(SPUsp45/EcoRV)

10µg

¥11,120.00

4

RT

 

 

 

VS-ELV01100

pNZ124Lactococcuslactiscloningvector

10µg

¥8,360.00

4

RT

 

 

 

VS-ELV01150

pNZ2105Lactococcuslactiscloningvector,foodgrade

10µg

¥8,760.00

4

RT

 

 

 

VS-ELV01200

pNZ2103Lactococcuslactisconst.vector,lacA

10µg

¥8,560.00

4

RT

 

 

 

VS-ELV01250

pNZ7021Lactococcuslactisconst.vector,pepN

10µg

¥8,560.00

4

RT

 

 

 

VS-ELV01300

pNZ2122Lactococcuslactisconst.vector,mcsX-H,foodgrade

10µg

¥8,960.00

4

RT

 

 

 

VS-ELV01350

pNZ2123Lactococcuslactisconst.vector,mcsH-X,foodgrade

10µg

¥8,960.00

4

RT

 

 

 

VS-ELV01400

pNZ2125Lactococcuslactisconst.vector,foodgrade,(alr)

10µg

¥8,960.00

4

RT

 

 

 

VS-ELV01450

pNZ7025Lactococcuslactisconst.vector,pepN,foodgrade,(alr)

10µg

¥8,960.00

4

RT

 

 

 

 

 

 

 

 

 

 

 

菌株Strains:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

VS-ELS10710-01

MC1061ChemicallyCompetent Escherichiacoli

5x100µl

¥7,960.00

80

DI

 

 

 

VS-ELS01363

LactococcuslactisexpressionstrainMG1363

1ml

¥5,340.00

80

DI

 

 

 

VS-ELS03000

LactococcuslactisexpressionStrainNZ3000,foodgrade

1ml

¥5,680.00

80

DI

 

 

 

VS-ELS03900-01

NICE®LactococcuslactisexpressionStrainNZ3900,foodgrade,(lacF)

1ml

¥5,340.00

80

DI

 

 

 

VS-ELS03910-01

NICE®LactococcuslactisexpressionStrainNZ3910,foodgrade,(lacF)

1ml

¥5,780.00

80

DI

 

 

 

VS-ELS09000-01

NICE®LactococcuslactisexpressionStrainNZ9000

1ml

¥5,340.00

80

DI

 

 

 

VS-ELS09100

NICE®LactococcuslactisexpressionStrainNZ9100

1ml

¥5,780.00

80

DI

 

 

 

VS-ELS09130-01

NICE®LactococcuslactisexpressionStrainNZ9130,foodgrade,(alr)

1ml

¥6,960.00

80

DI

 

 

 

VS-ELS10610-01

NICE®E.colihostStrainMC1061

1ml

¥5,540.00

80

DI

 

 

 

VS-ELS01330-01

NICE®LactococcuslactisexpressionStrainNZ1330,foodgrade,(alr)

1ml

¥6,960.00

80

DI

 

 

 

 

 

 

 

 

 

 

 

组合Mixed:

 

 

 

 

 

 

 

 

货号

产品描述

规格

报价

储存

运输

 

 

 

VS-ELV00500-01

NICE®pNZ9530LactococcuslactisnisRnisKvector,inStrainNZ9000

1ml

¥5,340.00

80

DI

 

 

 

 

 


成核剂,播种和定相试剂盒

PolyvalanCrystallophoreNo1-一种发光的complex络合物,是有效的核清洁剂,可提供明显的同构和异常定相能力。唯yi可以一次解决三个问题的化合物。

 

˚Forming:种植能够产生高质量的衍射数据的晶体

˚Finding:识别晶体滴快速,准确地

博士 ASING:在F“30层的电子对铽3+大号III边缘在1.67.5千电子伏  NaomiNucleants-NaomiNucleant促进了14种蛋白质的结晶,这是任何单个nucleant报道的zui高数量。这些蛋白质中的许多已被证明难以结晶,而其中一些蛋白质,包括膜蛋白质,仅在NaomiNucleant存在的情况下才结晶。

 

微种子珠 -微种子珠用于生成微种子-粉碎成碎片的微观晶体。

微晶播种的效果– IspD蛋白在微晶簇在液滴边缘成簇生长之前(左)和微晶种之后晶体分别生长(右)。(图片由TereseBergfors提供)。

 

含有3个碘原子的分子排列在等边三角形中,可用于建立SAD和SIRAS实验的实验阶段。

产品编号   产品名称   单位大小   单价   数量   

MD2-82 1号晶体电泳仪(小瓶,0.3mg)  0.3毫克    MOLECULARDIMENSIONS 1

MD2-80 1号晶体电泳仪(大瓶,0.6mg)  0.6毫克    MOLECULARDIMENSIONS 1

MD2-81 1号晶体荧光体(5个大的0.6mg小瓶)5x0.6毫克    MOLECULARDIMENSIONS   1

MD2-11 I3C相位套件1套 MOLECULARDIMENSIONS  1

MD2-07  娜奥米的努克利特   1个 MOLECULARDIMENSIONS   1

MD2-14  微种子珠(2.381毫米) 1x24珠    MOLECULARDIMENSIONS   1

MD9-25  细镊子 1个 MOLECULARDIMENSIONS   1



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