合肥MoBiTec GmbH试剂采购 MoBiTec ACE1双杂交系统

蚂蚁淘生物是一家专门从事抗体,蛋白,细胞等试剂产品研发与销售的公司.蚂蚁淘生物专业代理MoBiTecGmbHX 品牌试剂,大量现货,保证正品,价格优惠.欢迎来询

德国MoBiTec公司于1987年由StephanDiekmann教授创立，由于和Max-Planck协会（德国处于领先地位的研究中心）有着密切的关系，这使得本公司与很多具有创造性的科学家有着亲密的合作。另外，MoBiTec拥有来自欧洲和北美的研究人员，包括法国的Pasteur研究中心和荷兰的Vrije大学的科学家。MoBiTec公司能提供独一无二的高质量产品,从而简化实验设计，而留出更多更宝贵的时间用于实际的研究。
产品包括：双杂交和单杂交系统——体内蛋白-蛋白和DNA-蛋白关系，噬菌体展示系统——筛选特殊的多肽配体和选择具有特异性结合特性的新的蛋白质，LamdaPS基因文库，用于克隆的多功能DNA载体，靶基因的表达与分析，重组蛋白质高效表达和纯化试剂盒，细胞转染试剂和细胞培养工具，各种固体和溶解状态的酶，基因组学和蛋白组学研究产品（PCR，核酸，蛋白质纯化分析），各种抗体、细胞因子、生长因子、重组蛋白质，高级荧光试剂和试剂盒（蛋白质标记试剂盒，标记抗体，细胞染色试剂，钙指示器），亲和层析仪，凝胶分析工具——凝胶柱和凝胶电泳附件。
BacillusmegateriumExpressionSystem
BacillussubtilisExpressionVectors
pBacTagTaggingVectors
pPICHOLIShuttleVectorSystem
Broad-Host-RangeVectorspBBR122&pBHR1
FusionProteinCloningSystemPheBo
FusionProteinCloningSystempAX
BRPPlasmidandCompetentBRPCells
pPEPTIDECloningVector
Promoter-TrapVectorpBBRRESO
pORF-CLONEVectorSystem
ExontrapCloningVector
PCRCloningVectorp3T
SuicideVectorpCorrectClone
ssDNA-ProductionandExpressioninpMEX
His-tagCloningVectorpEG-His1
MultipleCloningSiteVectorpMCS5

YourpartnerforuniqueTwo-andOne-Hybrid-Systems,PhagemidDisplay,GenomicLibraries,DNAVectorSystems,CellBiologyandAntibodies,DNAandProteinTools,FluorescenceTechnology,AffinityChromatography,andLabSupplies

aboutMoBiTec
History

nnovativeToolsforMolecular&CellBiology

MoBiTecGmbHisaprivatelyheldcompanythatwasfoundedbyProf.Dr.StephanDiekmannin1987.ThecloserelationtotheMax-PlanckSocietyaswellasthelocationinGoettingen,oneofGermany’sleadingresearchcenters,facilitatedclosecooperationwithsomeofGermany’smostinventivescientists.Inaddition,MoBiTechasalwaysbeenoperatingextensivelywithscientistsalloverEuropeandNorthAmerica,includingthosefromthePasteurInstituteinFranceandVrijeUniversityintheNetherlands.Bycommercializingthebrilliantideasofmanyresearchers,MoBiTecisabletoofferauniquerangeofhighqualityproductswithsimplifiedprotocolsmakinglablifeeasierandleavingmoretimeforactualresearch.

Productsincludee.g.two-&onehybridsystemsforinvivodetectionofprotein-proteinorDNA-proteininteractions,aphagemiddisplaysystemforscreeningspecificpeptideligandsandselectingnovelproteinswithspecificbindingproperties,convenientLambdaPSgenomiclibraries;versatileDNAvectorsforcloning,expressingandanalyzingtargetgenes,kitsforefficientexpressionandpurificationofrecombinantproteins,celltransfectionreagentsandcellculturetools,immobilizedandsolubleenzymes,manyproductsforgenomicsandproteomicsresearch(e.g.forPCR,nucleicacid&proteinpurificationandanalysis),numerousantibodies,cytokines,growthfactorsandrecombinantproteins,superiorfluorescencereagentsandkits(e.g.proteinlabelingkits,labeledantibodies,cellstainingreagents,calciumindicators),affinitychromatographyproducts,gelanalysistoolsaswellasgenerallaboratoryequipmentsuchaslabcolumnsandgelelectrophoresisaccessories.

Inparalleltoitsownproductlines,MoBiTecdistributesproductsfromseveralinternationalcompaniesinGermanyincludingfluorescentprobesandresearchchemicals(MolecularProbes,AnaSpec,Teflabs),quantumdots(EvidentTechnologies),avarietyofDNApurificationkitsandDNApolymerases(EdgeBiosystems,TaKaRa),diagnostictools(Biomerica,Cytocell),gelelecrophoresisaccessories(Amresco),glycobiologyproducts(DextraLaboratories),high-qualitybiochipproducts(GenTel),celltransfectionreagents(Mirus)aswellasantibodies,proteins,substrates,finechemicalsandkitsformolecularandcellbiology-inparticularvirology,immunology,neurobiology,apoptosis,signaltransduction,cellproliferation,cytotoxicityandcancerresearch(MBL,A.G.Scientific,TaKaRa,Amresco,ViroGen,AnaSpec).

MoBiTecoffersatotalservice,beginningwiththeclearandfulldocumentationofproductsextendingtoindividualadviceandtechnicalserviceforcustomers.MoBiTecproductsaredistributedworldwide-inGermanyfromourhomeoffice,inothercountriesbydistributors.Moreover,MoBiTeciscontinuouslylookingfornewproductsandmarketabletechnologiesforlicensingandisthereforestronglyinterestedinscientificcollaborationsinordertodevelopinnovativeproductideas.

性能参数

产品名称：卓越性能，Mobitec巨大芽孢杆菌表达系统

英文名称： BacillusmegateriumExpressionSystems

产品型号： kit

产品规格： kit

产品产地：德国

品牌商标： MOBITEC

价格：面议元

产品详细描述

说明书(bmeg_handbook.pdf)

Features:

Stable,high-yieldproteinexpressionin Bacillusmegaterium
Idealforbothsmallandlarge-scaleproteinproduction
Tightlyregulatedandefficientlyinduciblexyloseoperon(upto350-fold)
Noalkalineproteasesactivityevenupto5hoursafterinduction
Noendotoxinsobserved
Versatilecloning(extendedpolylinker,additional BsrGIsite*)
Versatileproduction(intracellularorextracellular)
Versatilepurification(native,6xHis-tag,Strep-tag,Strep/6xHisdouble-tag)
Removeabletagversions(forTEVorXaproteases)available
Compatiblewithall B.subtilis vectors

*BsrGI siteisnotincludedinallvectors.

The B.megaterium expressionsystemprovidesaversatileandeasy-to-handletoolforstableandhigh-yieldproteinproduction,bothsmallandlargescale.

B.megaterium hasproventobeanexcellentalternativehostto E.coli forheterologousgeneexpression.Unlikeotherbacillistrains,proteolyticdegradationbyalkalineproteasesisavoided.Inaddition,therearenoendotoxinsfoundinthecellwall.

HighProteinYield
Asaresult,proteinyieldsareexceptionallyhighevenifinexpensivesubstratesareused.Forexample,theproteinsmutarotase(Mro)andglucosedehydrogenase(Gdh)havebeenaccumulatedupto20%and30%ofthetotalsolubleprotein,respectively.Usingthetightlyregulatedxyloseoperon,thegeneswereinduced130-to350-foldwithoutproteolysis.

VersatileSystemwithaWideRangeofVectors
MoBiTecprovidesawiderangeofusefulvectorsforthe B.megaterium systemthatareadaptableformostapplicationsandproteinpurificationmethods.Theseincludevectorscarryingasecretionsignalpeptidesequence,a6xHis-tag,aStrep-tag,andaStrep/6xHisdouble-tag.Additionally,allvectorscontainthetightlyregulatedandhighlyinduciblexyloseoperonpromoter.

B.megaterium protoplastsspecificallyoptimizedfortransformation
TheprotoplastssuppliedbyMoBiTecarefrom B.megaterium strainWH320developedbyProf.Dr.W.HillenattheInstituteofMicrobiologyinErlangen,Germany.Theseprotoplastsarepreparedaccordingtoanoptimizedprotocolresultinginthehighesttransformationefficiencies.
ForsecretionvectorsweofferprotoplastsofstrainMS941.BothstrainsaremutantsofDSM319,whereWH320isachemicallymutant,whileMS941hasadefineddeletioninthegeneofthemajorextracellularproteaseNprM.

Examples:

SelectedProteinssuccessfullyover-producedin Bacilli strainswithour B.megaterium vectors:

β-Galactosidase(LacZ)¹
Catabolitecontrolprotein(CcpA)^2,3
Clostridiumdifficile toxinA⁴
Cobaltochelatase(CbiX)⁵
Dextransucrase⁶
Endolevanase(LevB)⁷
Glucosedehydrogenase(GdhA)¹
Heatshockprotein(HPr)fromPTS(phosphotransferasesugartransportsystem)⁸
Humansingle-chainurokinase-likeplasminogenactivator(rscuPA)¹
Levansucrase⁹
Mutarotase(Mro)¹
Neopullulanase¹⁰
TranslocationATPaseofthepreproteintranslocase(SecA)¹¹
Trehaloserepressor(TreR)¹²

原代细胞培养试剂

细胞冷冻保存试剂

成像专用培养皿CG- 显微镜配件

神经元

特性

高纯度

高冻融活力

在培养约 2 天后，细胞将形成清晰的树突和轴突

描述

神经元，也被称为神经细胞，相互连接形成网络。它们是神经系统的主要组成部分，是研究神经系统功能的有力工具。

OriCell™ 神经元源自于大鼠或小鼠的海马或皮质，并作为原代细胞冷冻保存。其细菌、真菌和支原体检测结果呈阴性。

F344 胚胎大鼠海马神经元（订单号 RAWMX-01001）