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苏州蚂蚁淘生物科技有限公司专注于为科研工作者提供CST，Abcam，Sigma，biorbyt，Abclonal，AbDSerotec，GeneTex，ENZO，ScienCell，BI，Swant，emfret，Biovision，Roche，Millipore，BD，Life，eBioscience，Thermo，Qiagen，Santa，Peprotech，R&D，ImmunoStar，GenStar，GeneCopoeia，Cusabio，Bioss，NeoBioscience
bmeg_handbook.pdf)
*BsrGI siteisnotincludedinallvectors.
The B.megaterium expressionsystemprovidesaversatileandeasy-to-handletoolforstableandhigh-yieldproteinproduction,bothsmallandlargescale.
B.megaterium hasproventobeanexcellentalternativehostto E.coli forheterologousgeneexpression.Unlikeotherbacillistrains,proteolyticdegradationbyalkalineproteasesisavoided.Inaddition,therearenoendotoxinsfoundinthecellwall.
HighProteinYield
Asaresult,proteinyieldsareexceptionallyhighevenifinexpensivesubstratesareused.Forexample,theproteinsmutarotase(Mro)andglucosedehydrogenase(Gdh)havebeenaccumulatedupto20%and30%ofthetotalsolubleprotein,respectively.Usingthetightlyregulatedxyloseoperon,thegeneswereinduced130-to350-foldwithoutproteolysis.
VersatileSystemwithaWideRangeofVectors
MoBiTecprovidesawiderangeofusefulvectorsforthe B.megaterium systemthatareadaptableformostapplicationsandproteinpurificationmethods.Theseincludevectorscarryingasecretionsignalpeptidesequence,a6xHis-tag,aStrep-tag,andaStrep/6xHisdouble-tag.Additionally,allvectorscontainthetightlyregulatedandhighlyinduciblexyloseoperonpromoter.
B.megaterium protoplastsspecificallyoptimizedfortransformation
TheprotoplastssuppliedbyMoBiTecarefrom B.megaterium strainWH320developedbyProf.Dr.W.HillenattheInstituteofMicrobiologyinErlangen,Germany.Theseprotoplastsarepreparedaccordingtoanoptimizedprotocolresultinginthehighesttransformationefficiencies.
ForsecretionvectorsweofferprotoplastsofstrainMS941.BothstrainsaremutantsofDSM319,whereWH320isachemicallymutant,whileMS941hasadefineddeletioninthegeneofthemajorextracellularproteaseNprM.
SelectedProteinssuccessfullyover-producedin Bacilli strainswithour B.megaterium vectors:
MoBiTec品牌的载体表达系统的产品
一、 | 巨大芽孢杆菌系统 |
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| Bacillusmegaterium:availabletoacademicａndcommercialcustomer;commercialcustomersrequirealicensefromMoBiTec | ||||||||
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| 载体Vectors: |
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| 货号 | 产品描述 | 规格 | 报价 | 储存 | 运输 | |
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| BMEG03 | BacillusmegateriumvectorpWH1520,lyophilizedDNA | 5µg | ¥4,900.00 | 4度 | RT | |
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| BMEG10 | Bacillusmegateriumvector,pMM1522,lyophilizedDNA | 10µg | ¥4,900.00 | 4度 | RT | |
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| BMEG11 | Bacillusmegateriumvector,pMM1525,lyophilizedDNA | 10µg | ¥4,900.00 | 4度 | RT | |
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| BMEG12 | Bacillusmegateriumvector,pHIS1522,lyophilizedDNA | 10µg | ¥4,900.00 | 4度 | RT | |
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| BMEG13 | Bacillusmegateriumvector,pHIS1525,lyophilizedDNA | 10µg | ¥4,900.00 | 4度 | RT | |
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| BMEG13C | Levansucraseexpressionpositivecontrol,HIS-Tag | 10µg | ¥1,080.00 | 4度 | RT | |
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| BMEG14 | Bacillusmegateriumvector,pSTREP1525 | 10µg | ¥5,640.00 | 4度 | RT | |
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| BMEG14C | Levansucraseexpressionpositivecontrol,STREP-Tag | 10µg | ¥1,080.00 | 4度 | RT | |
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| BMEG15 | BacillusmegateriumvectorpSTREPHIS1525 | 10µg | ¥5,640.00 | 4度 | RT | |
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| BMEG15C | Levansucraseexpressionpositivecontrol,STREPHIS-Tag | 10µg | ¥1,080.00 | 4度 | RT | |
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| BMEG20 | BacillusmegateriumvectorpC-His1622 | 10µg | ¥6,840.00 | 4度 | RT | |
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| BMEG21 | BacillusmegateriumvectorpC-Strep1622 | 10µg | ¥8,420.00 | 4度 | RT | |
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| BMEG22 | BacillusmegateriumvectorpN-His-TEV1622 | 10µg | ¥6,840.00 | 4度 | RT | |
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| BMEG23 | BacillusmegateriumvectorpN-Strep-TEV1622 | 10µg | ¥8,420.00 | 4度 | RT | |
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| BMEG24 | BacillusmegateriumvectorpN-StrepXa1622 | 10µg | ¥8,420.00 | 4度 | RT | |
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| BMEG25 | Bacillusmegateriumvector,pS622,lyophilizedDNA. | 10µg | ¥6,840.00 | 4度 | RT | |
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| BMEG30 | Bacillusmegateriumvectorp3S623hp | 10µg | ¥19,360.00 | 4度 | RT | |
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| BMEG31 | BacillusmegateriumvectorpC-HIS1623hp | 10µg | ¥20,420.00 | 4度 | RT | |
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| BMEG32 | BacillusmegateriumvectorpN-HIS-TEV1623hp | 10µg | ¥20,420.00 | 4度 | RT | |
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| BMEG33 | BacillusmegateriumvectorpSP-LipA-hp | 10µg | ¥21,260.00 | 4度 | RT | |
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| BMEG34 | BacillusmegateriumvectorpSP-YocH-hp | 10µg | ¥21,260.00 | 4度 | RT | |
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| BMEG35 | Bacillusmegateriumvectorp3S623-2RBShp | 10µg | ¥20,000.00 | 4度 | RT | |
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| BMEG36 | BacillusmegateriumvectorpC-STREP1623hp | 10µg | ¥20,740.00 | 4度 | RT | |
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| BMEG37 | BacillusmegateriumvectorpN-STREP-Xa1623hp | 10µg | ¥20,940.00 | 4度 | RT | |
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| BMEG38 | BacillusmegateriumvectorpN-STREP-TEV1623hp | 10µg | ¥20,940.00 | 4度 | RT | |
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| BMEG10C | pGFP1522,GFPexpressionvector,positivecontrol | 10µg | ¥1,080.00 | 4度 | RT | |
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| BMEG39 | Bacillusmegateriumvector,pMGBm19,lyophilizedDNA | 10µg | ¥19,360.00 | 4度 | RT | |
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| BMEG40C | pGFP1624hp,highperformanceGFPexpressionvector,positivecontrol | 10µg | ¥9,680.00 | 4度 | RT | |
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| BMEG45 | BacillusmegateriumvectorpPconst1326 | 10µg | ¥11,260.00 | 4度 | RT | |
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| BMEGT710 | BacillusmegateriumpPT7cloningvector,lyophilized | 10µg | ¥11,260.00 | 4度 | RT | |
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| BMEGT710C | BacillusmegateriumpPT7-GFPcontrolvector,lyophilized | 10µg | ¥5,180.00 | 4度 | RT | |
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| BMEGT711 | BacillusmegateriumpPT7-SPlipAsecretionvector | 10ug | ¥12,320.00 | 4度 | RT | |
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| 菌株Strains: |
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| 货号 | 产品描述 | 规格 | 报价 | 储存 | 运输 | |
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| BMEG02 | Bacillusmegateriumprotoplasts,strainWH320 | 5x500µl | ¥7,580.00 | 负80度 | DI | |
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| BMEG04 | Bacillusmegateriumprotoplasts,strainYYBm1 | 5x500µl | ¥8,720.00 | 负80度 | DI | |
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| BMEG50 | Bacillusmegateriumprotoplasts,strainMS941 | 5x500µl | ¥8,440.00 | 负80度 | DI | |
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| 组合Mixed: |
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| 货号 | 产品描述 | 规格 | 报价 | 储存 | 运输 | |
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| BMEGT701 | BacillusmegateriumhighyieldT7geneexpressionkit,includespretransformedprotoplastsBMEGT702(5x500µl),pPT7cloningvectorａndpPT7-GFPcontrolvector(vectorslyophilized,10µgeach) | Kit | ¥20,780.00 | 负80度 | DI | |
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| BMEGT702 | Bacillusmegateriumprotoplasts,strainMS941,pretransformedwithpT7-RNAP | 5x500µL | ¥12,440.00 | 负80度 | DI | |
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二、 | 枯草芽孢杆菌 non-SURE |
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| Bacillussubtilisnon-SURE®:availabletoacademicａndcommercialcustomer,commercialcustomersrequirealicensefromMoBiTec | ||||||||
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| 载体Vectors: |
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| 货号 | 产品描述 | 规格 | 报价 | 储存 | 运输 | |
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| PBS001 | pHT01vector,lyophilizedplasmidDNA | 10µg | ¥8,040.00 | 4度 | RT | |
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| PBS001C | pHT01-bgaBcontrolvector,lyophilizedplasmidDNA,availableonlyincombinationwithregularvector | 10µg | ¥3,100.00 | 4度 | RT | |
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| PBS002 | pHT43vector,lyophilizedplasmidDNA | 10µg | ¥8,040.00 | 4度 | RT | |
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| PBS002C | pHT43-amyQcontrolvector,lyophilizedplasmidDNA,availableonlyincombinationwithregularvector | 10µg | ¥3,100.00 | 4度 | RT | |
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| PBS003 | pHT08vector,lyophilizedplasmidDNA | 10µg | ¥8,040.00 | 4度 | RT | |
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| PBS004 | pHT09vector,lyophilizedplasmidDNA | 10µg | ¥8,040.00 | 4度 | RT | |
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| PBS005 | pHT10vector,lyophilizedplasmidDNA | 10µg | ¥8,040.00 | 4度 | RT | |
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| PBS005C | pHT10-gfp+controlvector,lyophilizedplasmidDNA,availableonlyincombinationwithregularvector | 10µg | ¥3,100.00 | 4度 | RT | |
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| PBS013 | pHT253(pHT-Pgrac100-His-Tag-MCS) | 10µg | ¥9,620.00 | 4度 | RT | |
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| PBS014 | pHT254(pHT-Pgrac100-MCS-His-Tag) | 10µg | ¥9,620.00 | 4度 | RT | |
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| PBS015 | pHT255(pHT-Pgrac100-MCS-STREP-Tag) | 10µg | ¥9,620.00 | 4度 | RT | |
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| 菌株Strains: |
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| 货号 | 产品描述 | 规格 | 报价 | 储存 | 运输 | |
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| PBS020 | Bacillussubtilisstrain1012wt | 1mL | ¥8,460.00 | 负80度 | DI | |
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| PBS021 | Bacillussubtilisstrain168Marburg | 1mL | ¥6,360.00 | 负80度 | DI | |
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| PBS022 | BacillussubtilisstrainWB800N(forsecretionvectors) | 1mL | ¥11,140.00 | 负80度 | DI | |
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| PBS026 | BacillussubtilisstrainAS1 | 1mL | ¥9,300.00 | 负80度 | DI | |
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三、 | 枯草芽孢杆菌 SURE |
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| BacillussubtilisSURE®:availabletoacademiccustomersonly;commercialcustomerrequiretonegotiatewiththelicenserNIZO(Netherlands) | ||||||||
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| 载体Vectors: |
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| 货号 | 产品描述 | 规格 | 报价 | 储存 | 运输 | |
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| PBS031 | pNZ8901vector,lyophilizedDNA | 10µg | ¥9,620.00 | 4度 | RT | |
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| PBS032 | pNZ8902vector,lyophilizedDNA | 10µg | ¥9,620.00 | 4度 | RT | |
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| PBS033 | pNZ8910vector,lyophilizedDNA | 10µg | ¥9,620.00 | 4度 | RT | |
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| PBS034 | pNZ8911vector,lyophilizedDNA | 10µg | ¥9,620.00 | 4度 | RT | |
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| 菌株Strains: |
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| 货号 | 产品描述 | 规格 | 报价 | 储存 | 运输 | |
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| PBS023 | BacillussubtilisstrainNZ8963 | 1ml | ¥9,620.00 | 负80度 | DI | |
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| PBS024 | BacillussubtilisstrainNZ8900 | 1ml | ¥9,620.00 | 负80度 | DI | |
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| PBS025 | BacillussubtilisstrainNZ8901 | 1ml | ¥6,360.00 | 负80度 | DI | |
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四、 | 乳酸乳球菌的载体和菌株 |
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| Lactococcuslactisvectorsａndstrains: availabletoacademiccustomersonly;commercialcustomerrequiretonegotiatewiththelicenserNIZO(Netherlands) | ||||||||
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| 载体Vectors: |
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| 货号 | 产品描述 | 规格 | 报价 | 储存 | 运输 | |
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| VS-ELV00100-01 | NICE®pNZ8008ReferenceplasmidwithgusAgene | 10µg | ¥8,760.00 | 4度 | RT | |
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| VS-ELV00200-01 | NICE®pNZ8148Lactococcuslactisexpressionvector,NcoIsite | 10µg | ¥8,760.00 | 4度 | RT | |
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| VS-ELV00250-01 | NICE®pNZ8150Lactococcuslactisexpressionvector,ScaIsite | 10µg | ¥8,760.00 | 4度 | RT | |
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| VS-ELV00300-01 | NICE®pNZ8149Lactococcuslactisexpressionvector,foodgrade(lacF),NcoIsite | 10µg | ¥8,760.00 | 4度 | RT | |
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| VS-ELV00310-01 | NICE®pNZ8151Lactococcuslactisexpressionvector,foodgrade(lacF),Scalsite | 10µg | ¥8,760.00 | 4度 | RT | |
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| VS-ELV00400-01 | NICE®pNZ8152Lactococcuslactisexpressionvector,foodgrade(alr),Scalsite | 10µg | ¥8,760.00 | 4度 | RT | |
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| VS-ELV00500-01 | NICE®pNZ9530LactococcuslactisnisRnisKvector,inStrainNZ9000 | 1ml | ¥5,340.00 | 负80度 | DI | |
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| VS-ELV00600-01 | NICE®pNZ8120Lactococcuslactissecretionvector(SPPrtP/NaeI) | 10µg | ¥11,120.00 | 4度 | RT | |
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| VS-ELV00650-01 | NICE®pNZ8121Lactococcuslactissecretionvector(SPPrtP/EcoRV) | 10µg | ¥11,120.00 | 4度 | RT | |
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| VS-ELV00700-01 | NICE®pNZ8122Lactococcuslactissecretionvector(SPSlpA/NruI) | 10µg | ¥11,120.00 | 4度 | RT | |
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| VS-ELV00750-01 | NICE®pNZ8123Lactococcuslactissecretionvector(SPUsp45/NaeI) | 10µg | ¥11,120.00 | 4度 | RT | |
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| VS-ELV00800-01 | NICE®pNZ8124Lactococcuslactissecretionvector(SPUsp45/EcoRV) | 10µg | ¥11,120.00 | 4度 | RT | |
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| VS-ELV01100 | pNZ124Lactococcuslactiscloningvector | 10µg | ¥8,360.00 | 4度 | RT | |
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| VS-ELV01150 | pNZ2105Lactococcuslactiscloningvector,foodgrade | 10µg | ¥8,760.00 | 4度 | RT | |
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| VS-ELV01200 | pNZ2103Lactococcuslactisconst.vector,lacA | 10µg | ¥8,560.00 | 4度 | RT | |
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| VS-ELV01250 | pNZ7021Lactococcuslactisconst.vector,pepN | 10µg | ¥8,560.00 | 4度 | RT | |
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| VS-ELV01300 | pNZ2122Lactococcuslactisconst.vector,mcsX-H,foodgrade | 10µg | ¥8,960.00 | 4度 | RT | |
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| VS-ELV01350 | pNZ2123Lactococcuslactisconst.vector,mcsH-X,foodgrade | 10µg | ¥8,960.00 | 4度 | RT | |
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| VS-ELV01400 | pNZ2125Lactococcuslactisconst.vector,foodgrade,(alr) | 10µg | ¥8,960.00 | 4度 | RT | |
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| VS-ELV01450 | pNZ7025Lactococcuslactisconst.vector,pepN,foodgrade,(alr) | 10µg | ¥8,960.00 | 4度 | RT | |
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| 菌株Strains: |
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| 货号 | 产品描述 | 规格 | 报价 | 储存 | 运输 | |
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| VS-ELS10710-01 | MC1061ChemicallyCompetent Escherichiacoli | 5x100µl | ¥7,960.00 | 负80度 | DI | |
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| VS-ELS01363 | LactococcuslactisexpressionstrainMG1363 | 1ml | ¥5,340.00 | 负80度 | DI | |
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| VS-ELS03000 | LactococcuslactisexpressionStrainNZ3000,foodgrade | 1ml | ¥5,680.00 | 负80度 | DI | |
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| VS-ELS03900-01 | NICE®LactococcuslactisexpressionStrainNZ3900,foodgrade,(lacF) | 1ml | ¥5,340.00 | 负80度 | DI | |
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| VS-ELS03910-01 | NICE®LactococcuslactisexpressionStrainNZ3910,foodgrade,(lacF) | 1ml | ¥5,780.00 | 负80度 | DI | |
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| VS-ELS09000-01 | NICE®LactococcuslactisexpressionStrainNZ9000 | 1ml | ¥5,340.00 | 负80度 | DI | |
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| VS-ELS09100 | NICE®LactococcuslactisexpressionStrainNZ9100 | 1ml | ¥5,780.00 | 负80度 | DI | |
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| VS-ELS09130-01 | NICE®LactococcuslactisexpressionStrainNZ9130,foodgrade,(alr) | 1ml | ¥6,960.00 | 负80度 | DI | |
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| VS-ELS10610-01 | NICE®E.colihostStrainMC1061 | 1ml | ¥5,540.00 | 负80度 | DI | |
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| VS-ELS01330-01 | NICE®LactococcuslactisexpressionStrainNZ1330,foodgrade,(alr) | 1ml | ¥6,960.00 | 负80度 | DI | |
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| 组合Mixed: |
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| 货号 | 产品描述 | 规格 | 报价 | 储存 | 运输 | |
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| VS-ELV00500-01 | NICE®pNZ9530LactococcuslactisnisRnisKvector,inStrainNZ9000 | 1ml | ¥5,340.00 | 负80度 | DI | |
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| 酵母双(单)杂交系统: | Y2H膜蛋白系统;Grow""n""GlowGFP酵母单(双)杂交系统;Grow´n´GlowACE1酵母双杂交系统;Y2H膜蛋白系统cDNA文库;酵母载体;酵母菌;酵母转化和质粒提取;β-半乳糖苷酶活性检测试剂盒; |
| 噬菌体展示: | pSKAN噬菌体展示系统;Anti-plll(g3p)抗体;抗体;pSKAN8载体;pMAMPF3-PSTI4载体;LambdaPS噬菌体裂解物;Anti-hPSTI |
| 基因文库: | Y2HcDNA文库;LambdaPS基因文库; |