深圳MoBiTec公司代理生物计划 MoBiTec代理

苏州蚂蚁淘生物科技有限公司是专业从事生物技术产品销售的高科技企业，是国内生物试剂、仪器、消耗品领域的知名企业之一。蚂蚁淘公司自成立以来，连续2年高速成长。公司的发展目标是建成专业从事生物技术产品销售、生产与开发的一流公司。

德国MoBiTec公司于1987年由StephanDiekmann教授创立，由于和Max-Planck协会（德国处于领先地位的研究中心）有着密切的关系，这使得本公司与很多具有创造性的科学家有着亲密的合作。另外，MoBiTec拥有来自欧洲和北美的研究人员，包括法国的Pasteur研究中心和荷兰的Vrije大学的科学家。MoBiTec公司能提供独一无二的高质量产品,从而简化实验设计，而留出更多更宝贵的时间用于实际的研究。
产品包括：双杂交和单杂交系统——体内蛋白-蛋白和DNA-蛋白关系，噬菌体展示系统——筛选特殊的多肽配体和选择具有特异性结合特性的新的蛋白质，LamdaPS基因文库，用于克隆的多功能DNA载体，靶基因的表达与分析，重组蛋白质高效表达和纯化试剂盒，细胞转染试剂和细胞培养工具，各种固体和溶解状态的酶，基因组学和蛋白组学研究产品（PCR，核酸，蛋白质纯化分析），各种抗体、细胞因子、生长因子、重组蛋白质，高级荧光试剂和试剂盒（蛋白质标记试剂盒，标记抗体，细胞染色试剂，钙指示器），亲和层析仪，凝胶分析工具——凝胶柱和凝胶电泳附件。
BacillusmegateriumExpressionSystem
BacillussubtilisExpressionVectors
pBacTagTaggingVectors
pPICHOLIShuttleVectorSystem
Broad-Host-RangeVectorspBBR122&pBHR1
FusionProteinCloningSystemPheBo
FusionProteinCloningSystempAX
BRPPlasmidandCompetentBRPCells
pPEPTIDECloningVector
Promoter-TrapVectorpBBRRESO
pORF-CLONEVectorSystem
ExontrapCloningVector
PCRCloningVectorp3T
SuicideVectorpCorrectClone
ssDNA-ProductionandExpressioninpMEX
His-tagCloningVectorpEG-His1
MultipleCloningSiteVectorpMCS5

YourpartnerforuniqueTwo-andOne-Hybrid-Systems,PhagemidDisplay,GenomicLibraries,DNAVectorSystems,CellBiologyandAntibodies,DNAandProteinTools,FluorescenceTechnology,AffinityChromatography,andLabSupplies

aboutMoBiTec
History

nnovativeToolsforMolecular&CellBiology

MoBiTecGmbHisaprivatelyheldcompanythatwasfoundedbyProf.Dr.StephanDiekmannin1987.ThecloserelationtotheMax-PlanckSocietyaswellasthelocationinGoettingen,oneofGermany’sleadingresearchcenters,facilitatedclosecooperationwithsomeofGermany’smostinventivescientists.Inaddition,MoBiTechasalwaysbeenoperatingextensivelywithscientistsalloverEuropeandNorthAmerica,includingthosefromthePasteurInstituteinFranceandVrijeUniversityintheNetherlands.Bycommercializingthebrilliantideasofmanyresearchers,MoBiTecisabletoofferauniquerangeofhighqualityproductswithsimplifiedprotocolsmakinglablifeeasierandleavingmoretimeforactualresearch.

Productsincludee.g.two-&onehybridsystemsforinvivodetectionofprotein-proteinorDNA-proteininteractions,aphagemiddisplaysystemforscreeningspecificpeptideligandsandselectingnovelproteinswithspecificbindingproperties,convenientLambdaPSgenomiclibraries;versatileDNAvectorsforcloning,expressingandanalyzingtargetgenes,kitsforefficientexpressionandpurificationofrecombinantproteins,celltransfectionreagentsandcellculturetools,immobilizedandsolubleenzymes,manyproductsforgenomicsandproteomicsresearch(e.g.forPCR,nucleicacid&proteinpurificationandanalysis),numerousantibodies,cytokines,growthfactorsandrecombinantproteins,superiorfluorescencereagentsandkits(e.g.proteinlabelingkits,labeledantibodies,cellstainingreagents,calciumindicators),affinitychromatographyproducts,gelanalysistoolsaswellasgenerallaboratoryequipmentsuchaslabcolumnsandgelelectrophoresisaccessories.

Inparalleltoitsownproductlines,MoBiTecdistributesproductsfromseveralinternationalcompaniesinGermanyincludingfluorescentprobesandresearchchemicals(MolecularProbes,AnaSpec,Teflabs),quantumdots(EvidentTechnologies),avarietyofDNApurificationkitsandDNApolymerases(EdgeBiosystems,TaKaRa),diagnostictools(Biomerica,Cytocell),gelelecrophoresisaccessories(Amresco),glycobiologyproducts(DextraLaboratories),high-qualitybiochipproducts(GenTel),celltransfectionreagents(Mirus)aswellasantibodies,proteins,substrates,finechemicalsandkitsformolecularandcellbiology-inparticularvirology,immunology,neurobiology,apoptosis,signaltransduction,cellproliferation,cytotoxicityandcancerresearch(MBL,A.G.Scientific,TaKaRa,Amresco,ViroGen,AnaSpec).

MoBiTecoffersatotalservice,beginningwiththeclearandfulldocumentationofproductsextendingtoindividualadviceandtechnicalserviceforcustomers.MoBiTecproductsaredistributedworldwide-inGermanyfromourhomeoffice,inothercountriesbydistributors.Moreover,MoBiTeciscontinuouslylookingfornewproductsandmarketabletechnologiesforlicensingandisthereforestronglyinterestedinscientificcollaborationsinordertodevelopinnovativeproductideas.

性能参数

产品名称：卓越性能，Mobitec巨大芽孢杆菌表达系统

英文名称： BacillusmegateriumExpressionSystems

产品型号： kit

产品规格： kit

产品产地：德国

品牌商标： MOBITEC

价格：面议元

产品详细描述

说明书(bmeg_handbook.pdf)

Features:

Stable,high-yieldproteinexpressionin Bacillusmegaterium
Idealforbothsmallandlarge-scaleproteinproduction
Tightlyregulatedandefficientlyinduciblexyloseoperon(upto350-fold)
Noalkalineproteasesactivityevenupto5hoursafterinduction
Noendotoxinsobserved
Versatilecloning(extendedpolylinker,additional BsrGIsite*)
Versatileproduction(intracellularorextracellular)
Versatilepurification(native,6xHis-tag,Strep-tag,Strep/6xHisdouble-tag)
Removeabletagversions(forTEVorXaproteases)available
Compatiblewithall B.subtilis vectors

*BsrGI siteisnotincludedinallvectors.

The B.megaterium expressionsystemprovidesaversatileandeasy-to-handletoolforstableandhigh-yieldproteinproduction,bothsmallandlargescale.

B.megaterium hasproventobeanexcellentalternativehostto E.coli forheterologousgeneexpression.Unlikeotherbacillistrains,proteolyticdegradationbyalkalineproteasesisavoided.Inaddition,therearenoendotoxinsfoundinthecellwall.

HighProteinYield
Asaresult,proteinyieldsareexceptionallyhighevenifinexpensivesubstratesareused.Forexample,theproteinsmutarotase(Mro)andglucosedehydrogenase(Gdh)havebeenaccumulatedupto20%and30%ofthetotalsolubleprotein,respectively.Usingthetightlyregulatedxyloseoperon,thegeneswereinduced130-to350-foldwithoutproteolysis.

VersatileSystemwithaWideRangeofVectors
MoBiTecprovidesawiderangeofusefulvectorsforthe B.megaterium systemthatareadaptableformostapplicationsandproteinpurificationmethods.Theseincludevectorscarryingasecretionsignalpeptidesequence,a6xHis-tag,aStrep-tag,andaStrep/6xHisdouble-tag.Additionally,allvectorscontainthetightlyregulatedandhighlyinduciblexyloseoperonpromoter.

B.megaterium protoplastsspecificallyoptimizedfortransformation
TheprotoplastssuppliedbyMoBiTecarefrom B.megaterium strainWH320developedbyProf.Dr.W.HillenattheInstituteofMicrobiologyinErlangen,Germany.Theseprotoplastsarepreparedaccordingtoanoptimizedprotocolresultinginthehighesttransformationefficiencies.
ForsecretionvectorsweofferprotoplastsofstrainMS941.BothstrainsaremutantsofDSM319,whereWH320isachemicallymutant,whileMS941hasadefineddeletioninthegeneofthemajorextracellularproteaseNprM.

Examples:

SelectedProteinssuccessfullyover-producedin Bacilli strainswithour B.megaterium vectors:

β-Galactosidase(LacZ)¹
Catabolitecontrolprotein(CcpA)^2,3
Clostridiumdifficile toxinA⁴
Cobaltochelatase(CbiX)⁵
Dextransucrase⁶
Endolevanase(LevB)⁷
Glucosedehydrogenase(GdhA)¹
Heatshockprotein(HPr)fromPTS(phosphotransferasesugartransportsystem)⁸
Humansingle-chainurokinase-likeplasminogenactivator(rscuPA)¹
Levansucrase⁹
Mutarotase(Mro)¹
Neopullulanase¹⁰
TranslocationATPaseofthepreproteintranslocase(SecA)¹¹
Trehaloserepressor(TreR)¹²

成核剂，播种和定相试剂盒。

Polyvalan的CrystallophoreNo1-一种发光的complex络合物，是有效的核清洁剂，可提供明显的同构和异常定相能力。唯yi可以一次解决三个问题的化合物。

˚Forming：种植能够产生高质量的衍射数据的晶体

˚Finding：识别晶体滴快速，准确地

博士 ASING：在F“〜30层的电子对铽3+大号III边缘在1.6或7.5千电子伏 Naomi的Nucleants-Naomi的Nucleant促进了14种蛋白质的结晶，这是任何单个nucleant报道的zui高数量。这些蛋白质中的许多已被证明难以结晶，而其中一些蛋白质，包括膜蛋白质，仅在NaomiNucleant存在的情况下才结晶。

微种子珠 -微种子珠用于生成微种子-已“粉碎”成碎片的微观晶体。

微晶播种的效果– IspD蛋白在微晶簇在液滴边缘成簇生长之前（左）和微晶种之后晶体分别生长（右）。（图片由TereseBergfors提供）。

含有3个碘原子的分子排列在等边三角形中，可用于建立SAD和SIRAS实验的实验阶段。

产品编号产品名称单位大小单价数量

MD2-82 1号晶体电泳仪（小瓶，0.3mg） 0.3毫克 MOLECULARDIMENSIONS 1

MD2-80 1号晶体电泳仪（大瓶，0.6mg） 0.6毫克 MOLECULARDIMENSIONS 1

MD2-81 1号晶体荧光体（5个大的0.6mg小瓶）5x0.6毫克 MOLECULARDIMENSIONS 1

MD2-11 I3C相位套件1套 MOLECULARDIMENSIONS 1

MD2-07 娜奥米的努克利特 1个 MOLECULARDIMENSIONS 1

MD2-14 微种子珠（2.381毫米） 1x24珠 MOLECULARDIMENSIONS 1

MD9-25 细镊子 1个 MOLECULARDIMENSIONS 1