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广州进口MoBiTec GmbH试剂盒,血清清关 mobitec 质粒

苏州蚂蚁淘生物科技有限公司成立于2017年,是一家专业提供实验室整体采购方案的专业公司,为广大采购和实验室解决了大量问题,几年来已经成为所有合作伙伴最优秀的合作伙伴,我们一直秉承信誉第一的原则,坚持不卖假货,哪怕利润损失也要为客户提供最好的产品

我们已经和国外500多个品牌建立了贸易往来,并在国际上享有相当知名度,现已经成为国外近100多个品牌中国指定代理,并成为其中国最优秀的代理商


德国MoBiTec公司于1987年由Stephan Diekmann 教授创立,由于和Max-Planck协会(德国处于领先地位的研究中心)有着密切的关系,这使得本公司与很多具有创造性的科学家有着亲密的合作。另外,MoBiTec 拥有来自欧洲和北美的研究人员,包括法国的Pasteur 研究中心和荷兰的Vrije大学的科学家。MoBiTec公司能提供独一无二的高质量产品,从而简化实验设计,而留出更多更宝贵的时间用于实际的研究。
产品包括: 双杂交和单杂交系统——体内蛋白-蛋白和DNA-蛋白关系,噬菌体展示系统——筛选特殊的多肽配体和选择具有特异性结合特性的新的蛋白质,Lamda PS基因文库,用于克隆的多功能DNA载体,靶基因的表达与分析,重组蛋白质高效表达和纯化试剂盒,细胞转染试剂和细胞培养工具,各种固体和溶解状态的酶,基因组学和蛋白组学研究产品(PCR,核酸,蛋白质纯化分析),各种抗体、细胞因子、生长因子、重组蛋白质,高级荧光试剂和试剂盒(蛋白质标记试剂盒,标记抗体,细胞染色试剂,钙指示器),亲和层析仪,凝胶分析工具——凝胶柱和凝胶电泳附件。
Bacillus megaterium Expression System
Bacillus subtilis Expression Vectors
pBacTag Tagging Vectors
pPICHOLI Shuttle Vector System
Broad-Host-Range Vectors pBBR122 & pBHR1
Fusion Protein Cloning System PheBo
Fusion Protein Cloning System pAX
BRP Plasmid and Competent BRP Cells
pPEPTIDE Cloning Vector
Promoter-Trap Vector pBBR RESO
pORF-CLONE Vector System
Exontrap Cloning Vector
PCR Cloning Vector p3T
Suicide Vector pCorrectClone
ssDNA-Production and Expression in pMEX
His-tag Cloning Vector pEG-His1
Multiple Cloning Site Vector pMCS5

Your partner for unique Two- and One-Hybrid-Systems, Phagemid Display, Genomic Libraries, DNA Vector Systems, Cell Biology and Antibodies, DNA and Protein Tools, Fluorescence Technology, Affinity Chromatography, and Lab Supplies


about MoBiTec
History

nnovative Tools for Molecular & Cell Biology

 MoBiTec GmbH is a privately held company that was founded by Prof. Dr. Stephan Diekmann in 1987. The close relation to the Max-Planck Society as well as the location in Goettingen, one of Germany’s leading research centers, facilitated close cooperation with some of Germany’s most inventive scientists. In addition, MoBiTec has always been operating extensively with scientists all over Europe and North America, including those from the Pasteur Institute in France and Vrije University in the Netherlands. By commercializing the brilliant ideas of many researchers, MoBiTec is able to offer a unique range of high quality products with simplified protocols making lab life easier and leaving more time for actual research.

 

Products include e. g. two- & one hybrid systems for in vivo detection of protein-protein or DNA-protein interactions, a phagemid display system for screening specific peptide ligands and selecting novel proteins with specific binding properties, convenient Lambda PS genomic libraries; versatile DNA vectors for cloning, expressing and analyzing target genes, kits for efficient expression and purification of recombinant proteins, cell transfection reagents and cell culture tools, immobilized and soluble enzymes, many products for genomics and proteomics research (e. g. for PCR, nucleic acid & protein purification and analysis) , numerous antibodies, cytokines, growth factors and recombinant proteins, superior fluorescence reagents and kits (e. g. protein labeling kits, labeled antibodies, cell staining reagents, calcium indicators), affinity chromatography products, gel analysis tools as well as general laboratory equipment such as lab columns and gel electrophoresis accessories.

 

In parallel to its own product lines, MoBiTec distributes products from several international companies in Germany including fluorescent probes and research chemicals (Molecular Probes, AnaSpec, Teflabs), quantum dots (Evident Technologies), a variety of DNA purification kits and DNA polymerases (Edge Biosystems, TaKaRa), diagnostic tools (Biomerica, Cytocell), gel elecrophoresis accessories (Amresco), glycobiology products (Dextra Laboratories), high-quality biochip products (GenTel), cell transfection reagents (Mirus) as well as antibodies, proteins, substrates, fine chemicals and kits for molecular and cell biology - in particular virology, immunology, neurobiology, apoptosis, signal transduction, cell proliferation, cytotoxicity and cancer research (MBL, A.G. Scientific, TaKaRa, Amresco, ViroGen, AnaSpec).

 

MoBiTec offers a total service, beginning with the clear and full documentation of products extending to individual advice and technical service for customers. MoBiTec products are distributed worldwide - in Germany from our home office, in other countries by distributors. Moreover, MoBiTec is continuously looking for new products and marketable technologies for licensing and is therefore strongly interested in scientific collaborations in order to develop innovative product ideas.


性能参数

产品名称: 卓越性能,Mobitec巨大芽孢杆菌表达系统
英文名称: Bacillus megaterium Expression Systems
产品型号: kit
产品规格: kit
产品产地: 德国
品牌商标: MOBITEC
价    格: 面议元
 

产品详细描述

 

说明书(bmeg_handbook.pdf)

Features:

  • Stable, high-yield protein expression in Bacillus megaterium 
     
  • Ideal for both small and large-scale protein production
  • Tightly regulated and efficiently inducible xylose operon (up to 350-fold)
  • No alkaline proteases activity even up to 5 hours after induction
  • No endotoxins observed
  • Versatile cloning (extended polylinker, additional BsrG I site*)
  • Versatile production (intracellular or extracellular)
  • Versatile purification (native, 6xHis-tag, Strep-tag, Strep/6xHis double-tag)
  • Removeable tag versions (for TEV or Xa proteases) available New!
  • Compatible with all B. subtilis vectors

*BsrG I site is not included in all vectors.

The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small and large scale.

B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.

High Protein Yield
As a result, protein yields are exceptionally high even if inexpensive substrates are used. For example, the proteins mutarotase (Mro) and glucose dehydrogenase (Gdh) have been accumulated up to 20% and 30% of the total soluble protein, respectively. Using the tightly regulated xylose operon, the genes were induced 130- to 350-fold without proteolysis.


Versatile System with a Wide Range of Vectors
MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable for most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence, a 6xHis-tag, a Strep-tag, and a Strep/6xHis double-tag. Additionally, all vectors contain the tightly regulated and highly inducible xylose operon promoter.

B. megaterium protoplasts specifically optimized for transformation 
The protoplasts supplied by MoBiTec are from B. megaterium strain WH320 developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies. 

For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.

Examples:

Selected Proteins successfully over-produced in Bacilli strains with our B. megaterium vectors:

  • β-Galactosidase (LacZ)1
  • Catabolite control protein (CcpA)2,3
  • Clostridium difficile toxin A4
  • Cobaltochelatase (CbiX)5
  • Dextransucrase6
  • Endolevanase (LevB)7
  • Glucose dehydrogenase (GdhA)1
  • Heat shock protein (HPr) from PTS (phosphotransferase sugar transport system)8
  • Human single-chain urokinase-like plasminogen activator (rscuPA)1
  • Levansucrase9
  • Mutarotase (Mro)1
  • Neopullulanase10
  • Translocation ATPase of the preprotein translocase (SecA)11
  • Trehalose repressor (TreR)12

成核剂,播种和定相试剂盒。

PolyvalanCrystallophore N o 1-一种发光的complex络合物,是有效的核清洁剂,可提供明显的同构和异常定相能力。唯yi可以一次解决三个问题的化合物。

 

˚F orming:种植能够产生高质量的衍射数据的晶体

˚F inding:识别晶体滴快速,准确地

博士 ASING:在F“30层的电子对铽3+大号III边缘在1.67.5千电子伏  NaomiNucleants -NaomiNucleant促进了14种蛋白质的结晶,这是任何单个nucleant报道的zui高数量。这些蛋白质中的许多已被证明难以结晶,而其中一些蛋白质,包括膜蛋白质,仅在Naomi Nucleant存在的情况下才结晶。

 

微种子珠 -微种子珠用于生成微种子-粉碎成碎片的微观晶体。

微晶播种的效果– IspD蛋白在微晶簇在液滴边缘成簇生长之前(左)和微晶种之后晶体分别生长(右)。(图片由Terese Bergfors提供)。

 

含有3个碘原子的分子排列在等边三角形中,可用于建立SAD和SIRAS实验的实验阶段。

产品编号    产品名称    单位大小    单价    数量   

MD2-82  1号晶体电泳仪(小瓶,0.3mg)   0.3毫克    MOLECULARDIMENSIONS 1

MD2-80  1号晶体电泳仪(大瓶,0.6mg)   0.6毫克    MOLECULARDIMENSIONS 1

MD2-81  1号晶体荧光体(5个大的0.6mg小瓶) 5 x 0.6毫克    MOLECULARDIMENSIONS    1

MD2-11  I3C相位套件 1套 MOLECULARDIMENSIONS  1

MD2-07  娜奥米的努克利特    1个 MOLECULARDIMENSIONS    1

MD2-14  微种子珠(2.381毫米)  1 x 24珠    MOLECULARDIMENSIONS    1

MD9-25  细镊子  1个 MOLECULARDIMENSIONS    1


    苏州蚂蚁淘生物科技有限公司是一家经营医药标准品、化工环境标准品、高端试剂的专业公司,是全球领先科研方案的专业供应商。

  自成立以来,公司坚持以“保障客户利益是我们的第一追求”为经营宗旨,发展成为全球多个高端标准品公司国内总代理和授权代理商,能提供超过100000种的有机和无机标准品或试剂,产品涉及药企,科研单位,检测机构,工厂实验室和高校等绝大部分科研单位;同时提供优质的咨询服务,实力雄厚。

  公司以代理经营为主,代理20多个国外知名品牌的标准品、对照品,高端化学试剂,包括欧标EP,欧盟IRMM,日标JP,印度标IP,加拿大TRC,TLC,MC,英国BP,LGC,NIBSC,美国NIST,ChromaDex,美国Zyagen,挪威Chiron等。


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