企业QQ
官方微信
蚂蚁淘生物是一家专门从事抗体,蛋白,细胞等试剂产品研发与销售的公司.蚂蚁淘生物专业代理MoBiTecX 品牌试剂,大量现货,保证正品,价格优惠.欢迎来询
bmeg_handbook.pdf)
*BsrG I site is not included in all vectors.
The B. megaterium expression system provides a versatile and easy-to-handle tool for stable and high-yield protein production, both small and large scale.
B. megaterium has proven to be an excellent alternative host to E. coli for heterologous gene expression. Unlike other bacilli strains, proteolytic degradation by alkaline proteases is avoided. In addition, there are no endotoxins found in the cell wall.
High Protein Yield
As a result, protein yields are exceptionally high even if inexpensive substrates are used. For example, the proteins mutarotase (Mro) and glucose dehydrogenase (Gdh) have been accumulated up to 20% and 30% of the total soluble protein, respectively. Using the tightly regulated xylose operon, the genes were induced 130- to 350-fold without proteolysis.
Versatile System with a Wide Range of Vectors
MoBiTec provides a wide range of useful vectors for the B. megaterium system that are adaptable for most applications and protein purification methods. These include vectors carrying a secretion signal peptide sequence, a 6xHis-tag, a Strep-tag, and a Strep/6xHis double-tag. Additionally, all vectors contain the tightly regulated and highly inducible xylose operon promoter.
B. megaterium protoplasts specifically optimized for transformation
The protoplasts supplied by MoBiTec are from B. megaterium strain WH320 developed by Prof. Dr. W. Hillen at the Institute of Microbiology in Erlangen, Germany. These protoplasts are prepared according to an optimized protocol resulting in the highest transformation efficiencies.
For secretion vectors we offer protoplasts of strain MS941. Both strains are mutants of DSM319, where WH320 is a chemically mutant, while MS941 has a defined deletion in the gene of the major extracellular protease NprM.
Selected Proteins successfully over-produced in Bacilli strains with our B. megaterium vectors:
MoBiTec品牌的载体表达系统的产品
|
一、 |
巨大芽孢杆菌系统 |
|
|
|
|
|
|
||
|
|
Bacillus megaterium: available to academic ａnd commercial customer; commercial customers require a license from MoBiTec |
||||||||
|
|
|
载体Vectors: |
|
|
|
|
|
||
|
|
|
|
货号 |
产品描述 |
规格 |
报价 |
储存 |
运输 |
|
|
|
|
|
BMEG03 |
Bacillus megaterium vector pWH1520, lyophilized DNA |
5 µg |
¥4,900.00 |
4度 |
RT |
|
|
|
|
|
BMEG10 |
Bacillus megaterium vector, pMM1522, lyophilized DNA |
10 µg |
¥4,900.00 |
4度 |
RT |
|
|
|
|
|
BMEG11 |
Bacillus megaterium vector, pMM1525, lyophilized DNA |
10 µg |
¥4,900.00 |
4度 |
RT |
|
|
|
|
|
BMEG12 |
Bacillus megaterium vector, pHIS1522, lyophilized DNA |
10 µg |
¥4,900.00 |
4度 |
RT |
|
|
|
|
|
BMEG13 |
Bacillus megaterium vector, pHIS1525, lyophilized DNA |
10 µg |
¥4,900.00 |
4度 |
RT |
|
|
|
|
|
BMEG13C |
Levansucrase expression positive control, HIS-Tag |
10 µg |
¥1,080.00 |
4度 |
RT |
|
|
|
|
|
BMEG14 |
Bacillus megaterium vector, pSTREP1525 |
10 µg |
¥5,640.00 |
4度 |
RT |
|
|
|
|
|
BMEG14C |
Levansucrase expression positive control, STREP-Tag |
10 µg |
¥1,080.00 |
4度 |
RT |
|
|
|
|
|
BMEG15 |
Bacillus megaterium vector pSTREPHIS1525 |
10 µg |
¥5,640.00 |
4度 |
RT |
|
|
|
|
|
BMEG15C |
Levansucrase expression positive control, STREPHIS-Tag |
10 µg |
¥1,080.00 |
4度 |
RT |
|
|
|
|
|
BMEG20 |
Bacillus megaterium vector pC-His1622 |
10 µg |
¥6,840.00 |
4度 |
RT |
|
|
|
|
|
BMEG21 |
Bacillus megaterium vector pC-Strep1622 |
10 µg |
¥8,420.00 |
4度 |
RT |
|
|
|
|
|
BMEG22 |
Bacillus megaterium vector pN-His-TEV1622 |
10 µg |
¥6,840.00 |
4度 |
RT |
|
|
|
|
|
BMEG23 |
Bacillus megaterium vector pN-Strep-TEV1622 |
10 µg |
¥8,420.00 |
4度 |
RT |
|
|
|
|
|
BMEG24 |
Bacillus megaterium vector pN-StrepXa1622 |
10 µg |
¥8,420.00 |
4度 |
RT |
|
|
|
|
|
BMEG25 |
Bacillus megaterium vector, pS622, lyophilized DNA. |
10 µg |
¥6,840.00 |
4度 |
RT |
|
|
|
|
|
BMEG30 |
Bacillus megaterium vector p3S623hp |
10 µg |
¥19,360.00 |
4度 |
RT |
|
|
|
|
|
BMEG31 |
Bacillus megaterium vector pC-HIS1623hp |
10 µg |
¥20,420.00 |
4度 |
RT |
|
|
|
|
|
BMEG32 |
Bacillus megaterium vector pN-HIS-TEV1623hp |
10 µg |
¥20,420.00 |
4度 |
RT |
|
|
|
|
|
BMEG33 |
Bacillus megaterium vector pSP-LipA-hp |
10 µg |
¥21,260.00 |
4度 |
RT |
|
|
|
|
|
BMEG34 |
Bacillus megaterium vector pSP-YocH-hp |
10 µg |
¥21,260.00 |
4度 |
RT |
|
|
|
|
|
BMEG35 |
Bacillus megaterium vector p3S623-2RBShp |
10 µg |
¥20,000.00 |
4度 |
RT |
|
|
|
|
|
BMEG36 |
Bacillus megaterium vector pC-STREP1623hp |
10 µg |
¥20,740.00 |
4度 |
RT |
|
|
|
|
|
BMEG37 |
Bacillus megaterium vector pN-STREP-Xa1623hp |
10 µg |
¥20,940.00 |
4度 |
RT |
|
|
|
|
|
BMEG38 |
Bacillus megaterium vector pN-STREP-TEV1623hp |
10 µg |
¥20,940.00 |
4度 |
RT |
|
|
|
|
|
BMEG10C |
pGFP1522, GFP expression vector, positive control |
10 µg |
¥1,080.00 |
4度 |
RT |
|
|
|
|
|
BMEG39 |
Bacillus megaterium vector, pMGBm19, lyophilized DNA |
10 µg |
¥19,360.00 |
4度 |
RT |
|
|
|
|
|
BMEG40C |
pGFP1624hp, high performance GFP expression vector, positive control |
10 µg |
¥9,680.00 |
4度 |
RT |
|
|
|
|
|
BMEG45 |
Bacillus megaterium vector pPconst1326 |
10 µg |
¥11,260.00 |
4度 |
RT |
|
|
|
|
|
BMEGT710 |
Bacillus megaterium pPT7 cloning vector, lyophilized |
10 µg |
¥11,260.00 |
4度 |
RT |
|
|
|
|
|
BMEGT710C |
Bacillus megaterium pPT7-GFP control vector, lyophilized |
10 µg |
¥5,180.00 |
4度 |
RT |
|
|
|
|
|
BMEGT711 |
Bacillus megaterium pPT7-SPlipA secretion vector |
10 ug |
¥12,320.00 |
4度 |
RT |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
菌株Strains: |
|
|
|
|
|
||
|
|
|
|
货号 |
产品描述 |
规格 |
报价 |
储存 |
运输 |
|
|
|
|
|
BMEG02 |
Bacillus megaterium protoplasts, strain WH320 |
5x500 µl |
¥7,580.00 |
负80度 |
DI |
|
|
|
|
|
BMEG04 |
Bacillus megaterium protoplasts, strain YYBm1 |
5x500 µl |
¥8,720.00 |
负80度 |
DI |
|
|
|
|
|
BMEG50 |
Bacillus megaterium protoplasts, strain MS941 |
5x500 µl |
¥8,440.00 |
负80度 |
DI |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
组合Mixed: |
|
|
|
|
|
||
|
|
|
|
货号 |
产品描述 |
规格 |
报价 |
储存 |
运输 |
|
|
|
|
|
BMEGT701 |
Bacillus megaterium high yield T7 gene expression kit, includes pretransformed protoplasts BMEGT702 (5x500µl),pPT7 cloning vector ａnd pPT7-GFP control vector (vectors lyophilized, 10 µg each) |
Kit |
¥20,780.00 |
负80度 |
DI |
|
|
|
|
|
BMEGT702 |
Bacillus megaterium protoplasts, strain MS941, pretransformed with pT7-RNAP |
5x500 µL |
¥12,440.00 |
负80度 |
DI |
|
|
|
|
|
|
|
|
|
|
|
|
|
二、 |
枯草芽孢杆菌 non-SURE |
|
|
|
|
|
|||
|
|
Bacillus subtilis non-SURE®: available to academic ａnd commercial customer, commercial customers require a license from MoBiTec |
||||||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
载体Vectors: |
|
|
|
|
|
||
|
|
|
|
货号 |
产品描述 |
规格 |
报价 |
储存 |
运输 |
|
|
|
|
|
PBS001 |
pHT01 vector, lyophilized plasmid DNA |
10 µg |
¥8,040.00 |
4度 |
RT |
|
|
|
|
|
PBS001C |
pHT01-bgaB control vector, lyophilized plasmid DNA, available only in combination with regular vector |
10 µg |
¥3,100.00 |
4度 |
RT |
|
|
|
|
|
PBS002 |
pHT43 vector, lyophilized plasmid DNA |
10 µg |
¥8,040.00 |
4度 |
RT |
|
|
|
|
|
PBS002C |
pHT43-amyQ control vector, lyophilized plasmid DNA, available only in combination with regular vector |
10 µg |
¥3,100.00 |
4度 |
RT |
|
|
|
|
|
PBS003 |
pHT08 vector, lyophilized plasmid DNA |
10 µg |
¥8,040.00 |
4度 |
RT |
|
|
|
|
|
PBS004 |
pHT09 vector, lyophilized plasmid DNA |
10 µg |
¥8,040.00 |
4度 |
RT |
|
|
|
|
|
PBS005 |
pHT10 vector, lyophilized plasmid DNA |
10 µg |
¥8,040.00 |
4度 |
RT |
|
|
|
|
|
PBS005C |
pHT10-gfp+ control vector, lyophilized plasmid DNA, available only in combination with regular vector |
10 µg |
¥3,100.00 |
4度 |
RT |
|
|
|
|
|
PBS013 |
pHT253 (pHT-Pgrac100-His-Tag-MCS) |
10 µg |
¥9,620.00 |
4度 |
RT |
|
|
|
|
|
PBS014 |
pHT254 (pHT-Pgrac100-MCS-His-Tag) |
10 µg |
¥9,620.00 |
4度 |
RT |
|
|
|
|
|
PBS015 |
pHT255 (pHT-Pgrac100-MCS-STREP-Tag) |
10 µg |
¥9,620.00 |
4度 |
RT |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
菌株Strains: |
|
|
|
|
|
||
|
|
|
|
货号 |
产品描述 |
规格 |
报价 |
储存 |
运输 |
|
|
|
|
|
PBS020 |
Bacillus subtilis strain 1012wt |
1 mL |
¥8,460.00 |
负80度 |
DI |
|
|
|
|
|
PBS021 |
Bacillus subtilis strain 168 Marburg |
1 mL |
¥6,360.00 |
负80度 |
DI |
|
|
|
|
|
PBS022 |
Bacillus subtilis strain WB800N (for secretion vectors) |
1 mL |
¥11,140.00 |
负80度 |
DI |
|
|
|
|
|
PBS026 |
Bacillus subtilis strain AS1 |
1 mL |
¥9,300.00 |
负80度 |
DI |
|
|
|
|
|
|
|
|
|
|
|
|
|
三、 |
枯草芽孢杆菌 SURE |
|
|
|
|
|
|||
|
|
Bacillus subtilis SURE®: available to academic customers only; commercial customer require to negotiate with the licenser NIZO (Netherlands) |
||||||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
载体Vectors: |
|
|
|
|
|
||
|
|
|
|
货号 |
产品描述 |
规格 |
报价 |
储存 |
运输 |
|
|
|
|
|
PBS031 |
pNZ8901 vector, lyophilized DNA |
10 µg |
¥9,620.00 |
4度 |
RT |
|
|
|
|
|
PBS032 |
pNZ8902 vector, lyophilized DNA |
10 µg |
¥9,620.00 |
4度 |
RT |
|
|
|
|
|
PBS033 |
pNZ8910 vector, lyophilized DNA |
10 µg |
¥9,620.00 |
4度 |
RT |
|
|
|
|
|
PBS034 |
pNZ8911 vector, lyophilized DNA |
10 µg |
¥9,620.00 |
4度 |
RT |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
菌株Strains: |
|
|
|
|
|
||
|
|
|
|
货号 |
产品描述 |
规格 |
报价 |
储存 |
运输 |
|
|
|
|
|
PBS023 |
Bacillus subtilis strain NZ8963 |
1 ml |
¥9,620.00 |
负80度 |
DI |
|
|
|
|
|
PBS024 |
Bacillus subtilis strain NZ8900 |
1 ml |
¥9,620.00 |
负80度 |
DI |
|
|
|
|
|
PBS025 |
Bacillus subtilis strain NZ8901 |
1 ml |
¥6,360.00 |
负80度 |
DI |
|
|
|
|
|
|
|
|
|
|
|
|
|
四、 |
乳酸乳球菌的载体和菌株 |
|
|
|
|
|
|||
|
|
Lactococcus lactis vectors ａnd strains: available to academic customers only; commercial customer require to negotiate with the licenser NIZO (Netherlands) |
||||||||
|
|
|
|
|
|
|
|
|
|
|
|
|
|
载体Vectors: |
|
|
|
|
|
||
|
|
|
|
货号 |
产品描述 |
规格 |
报价 |
储存 |
运输 |
|
|
|
|
|
VS-ELV00100-01 |
NICE® pNZ8008 Reference plasmid with gusA gene |
10 µg |
¥8,760.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV00200-01 |
NICE® pNZ8148 Lactococcus lactis expression vector, NcoI site |
10 µg |
¥8,760.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV00250-01 |
NICE® pNZ8150 Lactococcus lactis expression vector, ScaI site |
10 µg |
¥8,760.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV00300-01 |
NICE® pNZ8149 Lactococcus lactis expression vector, food grade (lacF), NcoI site |
10 µg |
¥8,760.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV00310-01 |
NICE® pNZ8151 Lactococcus lactis expression vector, food grade (lacF), Scal site |
10 µg |
¥8,760.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV00400-01 |
NICE® pNZ8152 Lactococcus lactis expression vector, food grade (alr), Scal site |
10 µg |
¥8,760.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV00500-01 |
NICE® pNZ9530 Lactococcus lactis nisRnisK vector, in Strain NZ9000 |
1 ml |
¥5,340.00 |
负80度 |
DI |
|
|
|
|
|
VS-ELV00600-01 |
NICE® pNZ8120 Lactococcus lactis secretion vector (SP PrtP/NaeI) |
10 µg |
¥11,120.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV00650-01 |
NICE® pNZ8121 Lactococcus lactis secretion vector (SP PrtP/EcoRV) |
10 µg |
¥11,120.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV00700-01 |
NICE® pNZ8122 Lactococcus lactis secretion vector (SP SlpA/NruI) |
10 µg |
¥11,120.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV00750-01 |
NICE® pNZ8123 Lactococcus lactis secretion vector (SP Usp45/NaeI) |
10 µg |
¥11,120.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV00800-01 |
NICE® pNZ8124 Lactococcus lactis secretion vector (SP Usp45/EcoRV) |
10 µg |
¥11,120.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV01100 |
pNZ124 Lactococcus lactis cloning vector |
10 µg |
¥8,360.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV01150 |
pNZ2105 Lactococcus lactis cloning vector, food grade |
10 µg |
¥8,760.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV01200 |
pNZ2103 Lactococcus lactis const. vector, lacA |
10 µg |
¥8,560.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV01250 |
pNZ7021 Lactococcus lactis const. vector, pepN |
10 µg |
¥8,560.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV01300 |
pNZ2122 Lactococcus lactis const. vector, mcs X-H, food grade |
10 µg |
¥8,960.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV01350 |
pNZ2123 Lactococcus lactis const. vector, mcs H-X, food grade |
10 µg |
¥8,960.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV01400 |
pNZ2125 Lactococcus lactis const. vector, food grade, (alr) |
10 µg |
¥8,960.00 |
4度 |
RT |
|
|
|
|
|
VS-ELV01450 |
pNZ7025 Lactococcus lactis const. vector, pepN, food grade, (alr) |
10 µg |
¥8,960.00 |
4度 |
RT |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
菌株Strains: |
|
|
|
|
|
||
|
|
|
|
货号 |
产品描述 |
规格 |
报价 |
储存 |
运输 |
|
|
|
|
|
VS-ELS10710-01 |
MC1061 Chemically Competent Escherichia coli |
5 x 100 µl |
¥7,960.00 |
负80度 |
DI |
|
|
|
|
|
VS-ELS01363 |
Lactococcus lactis expression strain MG1363 |
1 ml |
¥5,340.00 |
负80度 |
DI |
|
|
|
|
|
VS-ELS03000 |
Lactococcus lactis expression Strain NZ3000, food grade |
1 ml |
¥5,680.00 |
负80度 |
DI |
|
|
|
|
|
VS-ELS03900-01 |
NICE® Lactococcus lactis expression Strain NZ3900, food grade, (lacF) |
1 ml |
¥5,340.00 |
负80度 |
DI |
|
|
|
|
|
VS-ELS03910-01 |
NICE® Lactococcus lactis expression Strain NZ3910, food grade, (lacF) |
1 ml |
¥5,780.00 |
负80度 |
DI |
|
|
|
|
|
VS-ELS09000-01 |
NICE® Lactococcus lactis expression Strain NZ9000 |
1 ml |
¥5,340.00 |
负80度 |
DI |
|
|
|
|
|
VS-ELS09100 |
NICE® Lactococcus lactis expression Strain NZ9100 |
1 ml |
¥5,780.00 |
负80度 |
DI |
|
|
|
|
|
VS-ELS09130-01 |
NICE® Lactococcus lactis expression Strain NZ9130, food grade, (alr) |
1 ml |
¥6,960.00 |
负80度 |
DI |
|
|
|
|
|
VS-ELS10610-01 |
NICE® E. coli host Strain MC1061 |
1 ml |
¥5,540.00 |
负80度 |
DI |
|
|
|
|
|
VS-ELS01330-01 |
NICE® Lactococcus lactis expression Strain NZ1330, food grade, (alr) |
1 ml |
¥6,960.00 |
负80度 |
DI |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
组合Mixed: |
|
|
|
|
|
||
|
|
|
|
货号 |
产品描述 |
规格 |
报价 |
储存 |
运输 |
|
|
|
|
|
VS-ELV00500-01 |
NICE® pNZ9530 Lactococcus lactis nisRnisK vector, in Strain NZ9000 |
1 ml |
¥5,340.00 |
负80度 |
DI |
|
|
|
|
|
|
|
|||||
成核剂，播种和定相试剂盒。
Polyvalan的Crystallophore N o 1-一种发光的complex络合物，是有效的核清洁剂，可提供明显的同构和异常定相能力。唯yi可以一次解决三个问题的化合物。
˚F orming：种植能够产生高质量的衍射数据的晶体
˚F inding：识别晶体滴快速，准确地
博士 ASING：在F“〜30层的电子对铽3+大号III边缘在1.6或7.5千电子伏 Naomi的Nucleants -Naomi的Nucleant促进了14种蛋白质的结晶，这是任何单个nucleant报道的zui高数量。这些蛋白质中的许多已被证明难以结晶，而其中一些蛋白质，包括膜蛋白质，仅在Naomi Nucleant存在的情况下才结晶。
微种子珠 -微种子珠用于生成微种子-已“粉碎”成碎片的微观晶体。
微晶播种的效果– IspD蛋白在微晶簇在液滴边缘成簇生长之前（左）和微晶种之后晶体分别生长（右）。（图片由Terese Bergfors提供）。
含有3个碘原子的分子排列在等边三角形中，可用于建立SAD和SIRAS实验的实验阶段。
产品编号 产品名称 单位大小 单价 数量
MD2-82 1号晶体电泳仪（小瓶，0.3mg） 0.3毫克 MOLECULARDIMENSIONS 1
MD2-80 1号晶体电泳仪（大瓶，0.6mg） 0.6毫克 MOLECULARDIMENSIONS 1
MD2-81 1号晶体荧光体（5个大的0.6mg小瓶） 5 x 0.6毫克 MOLECULARDIMENSIONS 1
MD2-11 I3C相位套件 1套 MOLECULARDIMENSIONS 1
MD2-07 娜奥米的努克利特 1个 MOLECULARDIMENSIONS 1
MD2-14 微种子珠（2.381毫米） 1 x 24珠 MOLECULARDIMENSIONS 1
MD9-25 细镊子 1个 MOLECULARDIMENSIONS 1
苏州蚂蚁淘生物科技有限公司是一家创新型高科技生物公司，以常规生物学实验外包与生物学试剂为基础，放眼于生命科学领域的前沿技术服务，公司技术骨干有着多年的生物学科研背景，毕业于国内外名牌大学，并且有着丰富的项目经验，了解国内外科研行情，我公司力求为每一个客户提专业、针对性的实验方案。我公司以技术服务为支点，和国内外多家生物试剂公司有着密切的业务往来.公司特色技术服务包括：抗体药物研发、噬菌体抗体库、蛋白表达与传化、抗体制备、高通量测序、组学研究、生物信息学分析与方法建立、基础分子生物学技术外包、化学中间体合成、原料药物研发、论文编写等。我公司秉承”诚信做人，认真做事，服务至上“，希望用我们的专业知识和项目经验为国内科研客户解决科研问题，祝您科研之路畅通无阻！